Gene Information

Name : cadA (PPUBIRD1_4929)
Accession : YP_005932685.1
Strain : Pseudomonas putida BIRD-1
Genome accession: NC_017530
Putative virulence/resistance : Resistance
Product : CadA
Function : -
COG functional category : -
COG ID : -
EC number : 3.6.3.3
Position : 5517278 - 5519542 bp
Length : 2265 bp
Strand : -
Note : heavy metal translocating P-type ATPase

DNA sequence :
ATGAACCAGCCTGTCAGCCACGAACACAAGCATCCGCACGATCACGATCACGATCACGCTCATGGCGATGACGACCATGG
CCATGCTGCCCACGCTCACACCTGCTGCGGCGCCAAAGCCGTGCCGCCGCTGGTGCAACTGAGCGAAACGGCCAGTGCCC
AGGCACAACTCAGTCGTTTCCGCATCGATGCCATGGACTGCCCCACCGAGCAGACCCTGATTCAGGACAAACTGAGCAAG
CTGGCCGGTATCGAACAGCTGGAATTCAACCTGATCAACCGCGTACTTGGCGTGCGCCACACCTTGGACGGCACCGCCGA
TATCGAGCGGGCTATCGACAGCCTGGGCATGAAGGCCGAGCCGATTGCCGCCCAGGACGACGGCAGCGTCAGCGTGCCAC
AACCGGCCAAGGCCCGCTGGTGGCCACTGGCATTGTCGGGTGTTGCCGCAATCGCCGCCGAAATCGTGCACTTTGCTGCA
CTCGCCCCGGAATGGATGGTGGCGGGCCTGGCACTGGCAGCGATCCTCGGTTGCGGCCTGGGTACCTACAAGAAGGGCTG
GATTGCCCTGAAAAACCGTAACCTCAACATCAATGCGCTGATGAGTATCGCTGTGACGGGTGCGGTACTGATCGGCCAGT
GGCCGGAAGCGGCTATGGTGATGGTGCTGTTCACCGTTGCCGAACTGATCGAAGCGCGTTCGCTGGACCGTGCGCGCAAT
GCCATTGGCGGGTTGATGCAACTGACCCCGGACATGGCCACCGTACAGCAGGCCGATGGCCAGTGGCGCGAGCTGGACGT
GCGTGAGGTGGCCGTCGGCGCACTGGTGCGGGTGCGCCCCGGTGAGCGTATCGGCCTGGACGGTGAAGTGACCAGCGGGC
AATCCAGCGTCGATCAGGCGCCGATTACCGGCGAAAGCCTGCCTGTGGAGAAGGGGGTGGGCGACAAGCTGTTCGCTGGC
ACCATCAACCAGGCGGGGGCGCTGGAGTTTCGCGTCACCGCCGCTGCCGGGCAGTCGACCCTGGCGCGGATCATCAAGGC
CGTGGAGGAAGCGCAGGGCGCGCGGGCGCCTACCCAACGCTTCGTCGACCGTTTCTCACGTATCTACACCCCGGTGGTGT
TCGCCATTGCCCTGGCCGTGGCGCTGATACCACCGCTGTTCATGGCTGGCGCCTGGTTCGATTGGATTTACCGCGCCTTG
GTGCTGCTGGTGGTGGCCTGCCCATGCGCGCTGGTGATCTCGACCCCGGTGACCATCGTCAGTGGCCTGGCCGCCGCTGC
GCGCAAAGGCATCCTGATCAAGGGTGGCGTGTATCTGGAGGGTGGCCGCCACCTGGATTTCCTGGCCCTGGACAAGACCG
GCACCATCACCCATGGCAAGCCGGTGCAGACCGACGCCAAGGTGCTGACGCCGCTGTTCGAAGACCGCGCACAAGCCTTG
GCGGCCAGCCTGGGCGAGCGTTCGGACCACCCGGTGTCCCGCGCTATTGCCGAGTTCGGCAAGCAGCAAGGCCTGGCCTT
GAGCGAGGTCAGTGACTTCGTTGCGCTGGCCGGGCGTGGCGTGCGTGGTGTCATCGCCGGTGAGGTCTACCACTTGGGCA
ACCATCGCCTGGTCGAGGAGCTGGGGCTGTGTTCACCAGCGCTGGAGGCCCAACTGGATGCGCTGGAGCGCCAGGGCAAG
ACCGTGGTCCTGCTGCTTGACCGCTCCGGGCCACTGGCACTGTTCGCTGTGGCCGACACGGTCAAGGACAGCAGCCGTCA
GGCGATTGCCGAATTGCATCAGCTGGGTATCAAGACTGTCATGCTGACCGGTGACAATCCGCATACTGCGCAGGCCATTG
CCGCCGTGGTGGGGATCGACCGCGCCGAGGGCAACCTGTTGCCGGCCGACAAGCTGAGAACCATTGAGGCGTTGTACGCC
CAAGGCCATCGGGTGGGCATGGTCGGTGACGGCATCAACGACGCCCCGGCGCTGGCCCGCGCCGAAATCGGTTTTGCCAT
GGCTGCTGCCGGCACCGACACCGCCATCGAGACGGCCGATGTGGCGCTGATGGATGATGACTTGCGCAAAATCCCGGCCT
TCGTCAGGCTGTCGCGCCAAAGTGCGGCGATCCTTATGCAGAACATCGTGCTGGCGTTGGGCATCAAGGCGATATTTCTG
GCGATCACCTTCGCCGGCATGGCCACGATGTGGATGGCGGTGTTCGCTGACATGGGCGTGAGCTTGCTGGTGGTGTTCAA
CGGCTTGCGCCTTTTGCGCAAATAG

Protein sequence :
MNQPVSHEHKHPHDHDHDHAHGDDDHGHAAHAHTCCGAKAVPPLVQLSETASAQAQLSRFRIDAMDCPTEQTLIQDKLSK
LAGIEQLEFNLINRVLGVRHTLDGTADIERAIDSLGMKAEPIAAQDDGSVSVPQPAKARWWPLALSGVAAIAAEIVHFAA
LAPEWMVAGLALAAILGCGLGTYKKGWIALKNRNLNINALMSIAVTGAVLIGQWPEAAMVMVLFTVAELIEARSLDRARN
AIGGLMQLTPDMATVQQADGQWRELDVREVAVGALVRVRPGERIGLDGEVTSGQSSVDQAPITGESLPVEKGVGDKLFAG
TINQAGALEFRVTAAAGQSTLARIIKAVEEAQGARAPTQRFVDRFSRIYTPVVFAIALAVALIPPLFMAGAWFDWIYRAL
VLLVVACPCALVISTPVTIVSGLAAAARKGILIKGGVYLEGGRHLDFLALDKTGTITHGKPVQTDAKVLTPLFEDRAQAL
AASLGERSDHPVSRAIAEFGKQQGLALSEVSDFVALAGRGVRGVIAGEVYHLGNHRLVEELGLCSPALEAQLDALERQGK
TVVLLLDRSGPLALFAVADTVKDSSRQAIAELHQLGIKTVMLTGDNPHTAQAIAAVVGIDRAEGNLLPADKLRTIEALYA
QGHRVGMVGDGINDAPALARAEIGFAMAAAGTDTAIETADVALMDDDLRKIPAFVRLSRQSAAILMQNIVLALGIKAIFL
AITFAGMATMWMAVFADMGVSLLVVFNGLRLLRK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ORF C97 AAN62190.1 putative metal-transporting P-type ATPase Not tested PAGI-2(C) Protein 0.0 65
unnamed BAB47609.1 cadmium resistance protein B Not tested Type-III SCCmec Protein 4e-110 48
cadA BAC57487.1 Probable cadmium-transporting ATPase (Cadmium efflux ATPase) Not tested Type-IIIinv SCCmec Protein 4e-110 48
SE0075 NP_763630.1 cadmium resistance protein B Not tested SCCpbp4 Protein 5e-108 47

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
cadA YP_005932685.1 CadA BAC0298 Protein 0.0 68
cadA YP_005932685.1 CadA BAC0455 Protein 4e-79 43
cadA YP_005932685.1 CadA BAC0461 Protein 1e-78 43
cadA YP_005932685.1 CadA BAC0092 Protein 2e-73 41