Gene Information

Name : hrcN (EPYR_03336)
Accession : YP_005804087.1
Strain : Erwinia pyrifoliae DSM 12163
Genome accession: NC_017390
Putative virulence/resistance : Virulence
Product : ATP synthase in type III secretion system
Function : -
COG functional category : -
COG ID : -
EC number : 3.6.3.14
Position : 3385091 - 3386455 bp
Length : 1365 bp
Strand : +
Note : ATP synthase subunit alpha (ATPase subunit alpha) (ATP synthase F1 sector subunit alpha), Type III secretion system ATPase, FliI/YscN, ATP synthase alpha/beta family, nucleotide-binding domain

DNA sequence :
ATGGTGATGAGCGCGCTACAGCAGCGCCTGACGCAGTGGGCAGACCAGCAGCAGCGCCGCCTGGAGCGCTACTCGCCCGT
TAGCCGCTACGGCCGTGTAACCGGTATCAGCGGCATTCTGATCGAGTGTATCCTGCCGGGCGCGCGCATCGGCGATTTAT
GCCGGATCCAACGCAGTGACGGCGGCAGCGTGCTGTCAGAGATCGTCGGCTTCAGCCCGGAGAAAATCCTGCTGTCGGCG
CTGGGCGCGCTGGACGGTATTTCCCAGGGTGCCACCATCGTCCCGCTTTATCTGCCGCACAGTATTTGCGTCAGTGAGCA
ACTGCTGGGCAGCGTGCTGGATGGTTTCGGCCGTGCGCTGGAGCCTGGCGGGCGCAGCGCTTTCGCCGAACCCGGATCCC
AGGTACGCGCCGTACCGGTACTGAACGATGCGCCGCCCCCTACCGAGCGCCCGCGTATTGCTACCCCGCTGCCGACCGGG
CTACGGGCGATTGACGGCCTGCTGACCATCGGTAACGGCCAGCGCGTGGGTATCTTTGCCGGAGCCGGCTGTGGCAAAAC
CACGCTGCTGGCGGAGCTGGCGCGCAACACCCCGTGCGATGCCATCGTCTTTGGCCTGATCGGCGAGCGTGGTCGTGAAC
TGCGCGAGTTTCTCGACCACGAGCTGGATGAAGAATTACGCAGCCGCACGGTACTGCTGTGCGCCACGTCGGATCGCAGC
AGTATGGAGCGCGCCCGCGCCGCATTTACCGCCACCGCCATCGCCGAGGCGTTTCGCGCCGAGGGCAAGAGCGTGCTGCT
GATCGTCGATTCACTCACCCGCTTTGCCCGCGCACAGCGTGAGATTGGCCTGGCGCTGGGCGAACCGCCCGGTCGTGGCG
GGCTGCCGCCATCGGTTTACACCCTGCTACCACGGCTGGTTGAACGCGCCGGGCAAACCCAACAGGGAGCGATTACCGCC
CTGTATTCGGTGCTGATCGAACAGGACTCAATGAACGACCCGGTGGCCGATGAAGTGCGTTCCCTGATCGACGGCCACAT
CGTGTTGAGCCGCCGTCTGGCCGAGCGCAACCACTATCCCGCCATCGATGTTTTAGCCAGCCTGAGCCGTACCATGAGCA
ACGTTGTTGAACCCCATCATATGATGCATGCCGGGCGGCTGCGCAGCCTGATGGCGGCGTATCAGCAGGTGGAAATGCTG
ATCCGCCTGGGTGAGTACCAGCCGGGGAATGACAGCCTGACCGATGCTGCCGTCAACGCCAATGACATCATCAACCAGTT
CCTGCGCCAGTCGATGCGCGCCCCCGATCCGTTTGAACAAACCCAGTATCACCTTGCGGAGGTCAGCGCCCATGCCCCTG
ATTGA

Protein sequence :
MVMSALQQRLTQWADQQQRRLERYSPVSRYGRVTGISGILIECILPGARIGDLCRIQRSDGGSVLSEIVGFSPEKILLSA
LGALDGISQGATIVPLYLPHSICVSEQLLGSVLDGFGRALEPGGRSAFAEPGSQVRAVPVLNDAPPPTERPRIATPLPTG
LRAIDGLLTIGNGQRVGIFAGAGCGKTTLLAELARNTPCDAIVFGLIGERGRELREFLDHELDEELRSRTVLLCATSDRS
SMERARAAFTATAIAEAFRAEGKSVLLIVDSLTRFARAQREIGLALGEPPGRGGLPPSVYTLLPRLVERAGQTQQGAITA
LYSVLIEQDSMNDPVADEVRSLIDGHIVLSRRLAERNHYPAIDVLASLSRTMSNVVEPHHMMHAGRLRSLMAAYQQVEML
IRLGEYQPGNDSLTDAAVNANDIINQFLRQSMRAPDPFEQTQYHLAEVSAHAPD

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 0.0 98
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 3e-134 71
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 1e-134 71
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 7e-135 71
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 1e-135 71
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 2e-115 69
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 7e-113 69
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 2e-125 68
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 1e-125 68
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 7e-123 67
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 7e-112 62
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 7e-112 62
lscN AAO18044.1 LscN Virulence TTSS locus Protein 1e-86 53
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 2e-73 51
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-73 51
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 1e-73 51
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 3e-74 51
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 8e-74 51
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 1e-79 49
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-78 49
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 1e-78 49
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-79 49
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 1e-79 49
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-79 49
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 1e-79 49
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 9e-76 49
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 9e-76 49
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 6e-72 47
escN NP_290268.1 hypothetical protein Virulence LEE Protein 1e-64 46
escN CAI43874.1 EscN protein Virulence LEE Protein 3e-65 46
ECs4568 NP_312595.1 EscN Virulence LEE Protein 1e-64 46
escN YP_003236088.1 translocator EscN Virulence LEE Protein 6e-65 46
escN AAC31513.1 L0034 Virulence LEE Protein 8e-65 46
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 8e-65 46
escN YP_003223475.1 translocator EscN Virulence LEE Protein 3e-65 46
escN AAC38383.1 EscN Virulence LEE Protein 8e-65 46
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 8e-69 45
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 8e-69 45
unnamed AAL06369.1 EscN Virulence LEE Protein 7e-64 45
escN CAC81862.1 EscN protein Virulence LEE II Protein 4e-65 45
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 6e-65 45
escN AAK26715.1 EscN Virulence LEE Protein 6e-65 45
escN AAL57542.1 EscN Virulence LEE Protein 6e-65 45
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 1e-59 44
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 2e-59 44
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 2e-59 44
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 2e-59 44
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 2e-59 44
invC AAS66861.1 InvC Not tested SSR-2 Protein 1e-58 43
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 9e-59 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
hrcN YP_005804087.1 ATP synthase in type III secretion system VFG0048 Protein 3e-87 53
hrcN YP_005804087.1 ATP synthase in type III secretion system VFG0390 Protein 7e-84 53
hrcN YP_005804087.1 ATP synthase in type III secretion system VFG0192 Protein 2e-87 53
hrcN YP_005804087.1 ATP synthase in type III secretion system VFG0515 Protein 4e-74 51
hrcN YP_005804087.1 ATP synthase in type III secretion system VFG1254 Protein 5e-67 47
hrcN YP_005804087.1 ATP synthase in type III secretion system VFG2144 Protein 2e-80 46
hrcN YP_005804087.1 ATP synthase in type III secretion system VFG0812 Protein 4e-65 46
hrcN YP_005804087.1 ATP synthase in type III secretion system VFG0730 Protein 4e-65 46
hrcN YP_005804087.1 ATP synthase in type III secretion system VFG1008 Protein 9e-59 45
hrcN YP_005804087.1 ATP synthase in type III secretion system VFG0555 Protein 8e-60 44
hrcN YP_005804087.1 ATP synthase in type III secretion system VFG1778 Protein 1e-60 44
hrcN YP_005804087.1 ATP synthase in type III secretion system VFG2459 Protein 1e-61 43
hrcN YP_005804087.1 ATP synthase in type III secretion system VFG2331 Protein 1e-64 42
hrcN YP_005804087.1 ATP synthase in type III secretion system VFG2499 Protein 6e-62 41