Gene Information

Name : spaI (EPYR_02150)
Accession : YP_005802901.1
Strain : Erwinia pyrifoliae DSM 12163
Genome accession: NC_017390
Putative virulence/resistance : Virulence
Product : type III secretion system ATPase SpaI/InvC
Function : -
COG functional category : -
COG ID : -
EC number : 3.6.3.14
Position : 2245735 - 2246976 bp
Length : 1242 bp
Strand : -
Note : ATP synthase subunit alpha (ATPase subunit alpha) (ATP synthase F1 sector subunit alpha), Type III secretion system ATPase, FliI/YscN

DNA sequence :
GTGATAGAAGCATACCTTCCAGGAACGAAGATTGGAGAAATTTGTCAAATCCAGAAATCATTAGTTGAAAGTGAAGTTTT
AGGACTCGCGCAAATAACCGGATTTAATAATGAACATACATTTTTAAGCCTCTTAACGGATAGTCAGGGTTTTTCACGAG
AAAACGTATTGTTGCCAACGGGTAAGCCTCTGCGCGTTGAGGTTCATGAGGGCATAAAAGGGGCCATCATTGATGCTACT
GGAACGATCAAAGGGCATCTGGTTGATATGCCTGTTACTCCTTACACAAATGTAAAGAGTATTATGGCCTGCGGTTCGGC
GCGTGATTTTAGCTTACGTAAACCAATAACAGAGCCATTGTTAACCGGAATTCGTGCCATTGATGGTTTGCTTACCTGTG
GTCAGGGACAACGAATCGGAATTTTTGCGACGGCTGGCTGTGGTAAAACATCGCTGATGAATATGCTAATCGAGCATTCA
GAAGCGGATATCTATGTTATCGGCCTGATAGGTGAACGAGGACGAGAAGTAACTGAATTCGTCGAATCAATGAAAAAGTC
TTCAAGACGTTCAAGGATCGTAATAGTTTATGCTACCTCGGACCGCTCTTCGGTGGAACGTAGTAATGCCGCACAAATTG
CATCAAGTATTGCCGAGTATTTCAGTGAGTTGGGCAACAATGTACTACTTTTTGTGGATTCGATCACCCGTTACGCCCGT
GCGTTGCGTGACGTTGCACTGAGTATGGGGGAAATGCCCGTGCGTCGCGGCTATCCTGCATCAGTGTTTGAGGCGTTACC
AAAGCTGCTCGAGCGGCCTGGTCGTTTCCTCACCGGATCAATAACAGCATTTTATACTGTGCTAATTGAAAACGAAGAAG
ATCCAGATGTAATCGGTGATGAAGTGCGCTCGATTTTGGATGGACATATCTACCTGAGCCGTAAACTCGCCGCTAAGGGG
CATTATCCGGCCATTAATGTACTTAACAGTATTAGCAGAGTTTTTTCACAAGTAACAGAACGAAATCAGCAGCAAATGGC
GAACTGCTTCCGTGATTTATTACAGCGTCAACGTGATATGCAGCTATTTATTGATTTAGGCGAATATCACCGGGGTGAAA
ATGAGAATAATGATATTGCTTTTGATAAGAACGAACAAATGGAAGAATTTCTCAAACAAGATATGACAGAAAAAATGGAA
ATTTCGACCTGCATGGATATGTTATATGGATGCGTGGCATAA

Protein sequence :
MIEAYLPGTKIGEICQIQKSLVESEVLGLAQITGFNNEHTFLSLLTDSQGFSRENVLLPTGKPLRVEVHEGIKGAIIDAT
GTIKGHLVDMPVTPYTNVKSIMACGSARDFSLRKPITEPLLTGIRAIDGLLTCGQGQRIGIFATAGCGKTSLMNMLIEHS
EADIYVIGLIGERGREVTEFVESMKKSSRRSRIVIVYATSDRSSVERSNAAQIASSIAEYFSELGNNVLLFVDSITRYAR
ALRDVALSMGEMPVRRGYPASVFEALPKLLERPGRFLTGSITAFYTVLIENEEDPDVIGDEVRSILDGHIYLSRKLAAKG
HYPAINVLNSISRVFSQVTERNQQQMANCFRDLLQRQRDMQLFIDLGEYHRGENENNDIAFDKNEQMEEFLKQDMTEKME
ISTCMDMLYGCVA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 5e-123 63
invC AAS66861.1 InvC Not tested SSR-2 Protein 7e-110 55
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 5e-105 55
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 5e-105 55
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 5e-105 55
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 5e-105 55
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 4e-101 53
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 1e-69 45
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 9e-73 44
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 9e-73 44
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 8e-73 44
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 3e-73 44
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 6e-73 44
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 9e-75 44
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 9e-68 43
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 1e-63 43
lscN AAO18044.1 LscN Virulence TTSS locus Protein 2e-80 43
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 1e-67 43
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 1e-67 43
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 1e-67 43
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 2e-69 43
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 1e-69 43
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 6e-75 43
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-74 43
escN AAC31513.1 L0034 Virulence LEE Protein 2e-66 42
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 2e-66 42
unnamed AAL06369.1 EscN Virulence LEE Protein 3e-64 42
escN AAC38383.1 EscN Virulence LEE Protein 2e-66 42
escN AAK26715.1 EscN Virulence LEE Protein 9e-66 42
escN AAL57542.1 EscN Virulence LEE Protein 9e-66 42
escN YP_003236088.1 translocator EscN Virulence LEE Protein 1e-65 42
escN CAC81862.1 EscN protein Virulence LEE II Protein 6e-66 42
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 8e-66 42
escN CAI43874.1 EscN protein Virulence LEE Protein 5e-66 42
escN YP_003223475.1 translocator EscN Virulence LEE Protein 7e-66 42
escN NP_290268.1 hypothetical protein Virulence LEE Protein 3e-66 42
ECs4568 NP_312595.1 EscN Virulence LEE Protein 3e-66 42
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-75 42
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-75 42
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 4e-68 42
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 2e-67 42
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 1e-67 42
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 7e-75 42
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-74 42
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 3e-74 42
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 7e-75 42
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 9e-75 42
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 1e-70 41
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 3e-66 41
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 3e-66 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
spaI YP_005802901.1 type III secretion system ATPase SpaI/InvC VFG1778 Protein 3e-106 57
spaI YP_005802901.1 type III secretion system ATPase SpaI/InvC VFG2459 Protein 1e-112 55
spaI YP_005802901.1 type III secretion system ATPase SpaI/InvC VFG1008 Protein 4e-101 55
spaI YP_005802901.1 type III secretion system ATPase SpaI/InvC VFG0555 Protein 2e-105 55
spaI YP_005802901.1 type III secretion system ATPase SpaI/InvC VFG0048 Protein 2e-77 45
spaI YP_005802901.1 type III secretion system ATPase SpaI/InvC VFG0390 Protein 2e-78 44
spaI YP_005802901.1 type III secretion system ATPase SpaI/InvC VFG0515 Protein 3e-73 44
spaI YP_005802901.1 type III secretion system ATPase SpaI/InvC VFG2144 Protein 3e-72 44
spaI YP_005802901.1 type III secretion system ATPase SpaI/InvC VFG0192 Protein 7e-82 43
spaI YP_005802901.1 type III secretion system ATPase SpaI/InvC VFG0730 Protein 1e-66 42
spaI YP_005802901.1 type III secretion system ATPase SpaI/InvC VFG0812 Protein 1e-66 42