Gene Information

Name : Clo1313_2609 (Clo1313_2609)
Accession : YP_005689060.1
Strain : Clostridium thermocellum DSM 1313
Genome accession: NC_017304
Putative virulence/resistance : Resistance
Product : D-alanine--D-alanine ligase
Function : -
COG functional category : -
COG ID : -
EC number : 6.3.2.4
Position : 3074441 - 3075571 bp
Length : 1131 bp
Strand : -
Note : TIGRFAM: D-alanine/D-alanine ligase; KEGG: cce:Ccel_0283 D-alanyl-alanine synthetase A; PFAM: D-alanine--D-alanine ligase domain protein

DNA sequence :
ATGGGCGATAAAAAAAGAGTACTGGTTATTTTTGGCGGACAATCGTCGGAACACGAAGTTTCAAGAATATCGGCAACATC
CATACTGAAGAACATTAATTTGGATAAATTCGATGTTTCAATGATAGGAATCACAAAAGACGGAAAGTGGCTTTATTATG
ACGGGCCTATTGATAAAATTCCTTCCGGAGAGTGGGAGGAAATCGCACTGAAAGATGGGACAAGAAGTATTGCCGACAGA
GTGAGCCTGTTCGACAACATAATAAGTTGCAAAAATAACGCATGCGGCCTTGAAAAGGCCTCAGAGAACGAAAAAAGCAA
AAAGATAGATGTGGTTTTTCCGGTTCTGCACGGCTGCAACGGTGAAGACGGGACCATCCAGGGACTTTTTGAACTGGCGG
GCATTCCTTATGTGGGCTGCGGTGTGCTGGCTTCAGCAGTCGGAATGGATAAGATTTATGCAAAGATAATTTTTGAAAAA
GCCGGAATACCCCAGGCGGATTATCTGTATTTCACAAGAAAAGAAATTTACGGGGATGTTGAGGGTGTGGTTGACAAAAT
AGAGGAGAAATTTTCATATCCTGTATTTGTAAAACCGTCCAATGCCGGTTCTTCCGTAGGTGTGTCAAAGGCGCATGATA
AAAATGAGCTTAAAGAGGCATTGATTTATGCCGCCAGGTATGATAGAAAAGTACTGATTGAGGAATTTATCAACGGAAGA
GAAGTTGAGTGTGCCGTGCTGGGGAATGATGATCCTGTGGCATCAACGGTGGGAGAAATCATTCCGGGAAATGAATTTTA
CGACTACAAGGCAAAATACATTGACAATACTTCCAAAATAAAAATTCCCGCGGATCTTCCAGAAGAGACCGTGGAACAAA
TAAGAAATTATGCAGTAAAGGCATTCAAGGCTTTGGATTGTTCGGGACTTGCAAGAGTTGACTTTTTTGTGCACAAGGAA
ACCGGAAAAGTTTATATAAATGAAATTAATACAATGCCGGGATTTACAAGTATAAGCATGTATCCCATGCTTTGGGAGGA
ATCCGGCATTTCCTATCCGGAACTTATTGAAAAGCTGATTGACTTGGCTGTTCAAAGATACAATGACAATCTCAAAGAAT
ATGATGAGTAG

Protein sequence :
MGDKKRVLVIFGGQSSEHEVSRISATSILKNINLDKFDVSMIGITKDGKWLYYDGPIDKIPSGEWEEIALKDGTRSIADR
VSLFDNIISCKNNACGLEKASENEKSKKIDVVFPVLHGCNGEDGTIQGLFELAGIPYVGCGVLASAVGMDKIYAKIIFEK
AGIPQADYLYFTRKEIYGDVEGVVDKIEEKFSYPVFVKPSNAGSSVGVSKAHDKNELKEALIYAARYDRKVLIEEFINGR
EVECAVLGNDDPVASTVGEIIPGNEFYDYKAKYIDNTSKIKIPADLPEETVEQIRNYAVKAFKALDCSGLARVDFFVHKE
TGKVYINEINTMPGFTSISMYPMLWEESGISYPELIEKLIDLAVQRYNDNLKEYDE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
vanB NP_815951.1 D-alanine--D-lactate ligase Not tested Not named Protein 7e-55 42
vanB YP_008394259.1 Vancomycin B-type resistance protein vanB, D-alanine--D-lactate ligase Not tested Not named Protein 4e-54 41

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Clo1313_2609 YP_005689060.1 D-alanine--D-alanine ligase AE016830.1.gene2250. Protein 3e-55 42
Clo1313_2609 YP_005689060.1 D-alanine--D-alanine ligase U00456.gene.p01 Protein 3e-55 42
Clo1313_2609 YP_005689060.1 D-alanine--D-alanine ligase U35369.1.gene6.p01 Protein 3e-55 42
Clo1313_2609 YP_005689060.1 D-alanine--D-alanine ligase AY145441.gene.p01 Protein 9e-55 41