Gene Information

Name : polA-2 (CBF_3058)
Accession : YP_005675860.1
Strain : Clostridium botulinum 230613
Genome accession: NC_017297
Putative virulence/resistance : Unknown
Product : DNA polymerase I
Function : -
COG functional category : -
COG ID : -
EC number : 2.7.7.7
Position : 3244431 - 3247058 bp
Length : 2628 bp
Strand : -
Note : identified by match to protein family HMM PF00476; match to protein family HMM PF01367; match to protein family HMM PF02739; match to protein family HMM TIGR00593

DNA sequence :
ATGAATAAAGAAAGATTATTAATATTAGATAGTAATAGTTTATTAAATAGAGCTTTTTATGCATTACCTGATCTTATGAC
TGGAGAGGGAATTCATACTAATGCTATATATGGTTTTGTTAATATGCTTTTAAAAATGAAAGAAGAGATAAACCCAGATT
ATATAGTTGCAGCTTTTGATAGAAAGGCACCTACCTTTAGACATGAAGAATATAAGGACTATAAAGCAGGTAGAAAAAAG
ATGCCAGAGGAACTTGCACAGCAGTTTCCTATAATAAAAGAGCTTTTGTCCGCATTAGCCATAAATATATTCGAAATTGA
TGGATTTGAAGCAGATGATCTAATAGGAACTTTATCTGTATTTGCAGAGGAAAAAGGAATAGAAGTTTACATAGTTACAG
GAGATAAAGATGCCCTACAATTGGCTACGGATAGCGTAAAAGTAGTTATAACTAAAAAGGGTATTACAGAAAAAGAAATT
TATGATAAAAATAGAATGGTAGAGGAGTTTGGAGTTACTCCTAAAGAATTTATAGATGTAAAAGGTCTTATGGGAGACAC
ATCTGACAATATACCTGGAGTACCTGGAATAGGTGAAAAAACTGCCTTTAAGCTTATAAAAGAATATAAAAGTATAGAAA
CAGTATTTGAAAACATAGAGAATATAAAAGGAAAAAAATTAAAGGAAAATTTACATGAATATAGAGAGCAGGCTATATTT
AGCAAAAAATTAGCTACTATTATATGTAATGTTCCTATAGATATGAGTTTAGAAGATATAAAATCAAAGGAAGAATATGA
TATAAACAAAGTTAGAGAAATGTTTGAAAGGTTAGAATTTAAATCTTTAATGAATAAAGTAGGTAAAGGTAATGAGGGAG
AAACTGTAGAAGAGGAAGTCTCTTATAAAAATATAGTTTCTCTAGAAGAATTTAGAGATCTAAAAAATAATATAATAAAA
TATAAAGAAAATGATTTATATCTGTATTTTGAATTAGAAGATATAGTTTTATTTTCTAAATCTAGAGTAAAAACATTATA
TGCTAATTTTAAAGATGAGGTTTATAGAATAGACTTTGAAATGTTGCTAAATAATACTAAAGAACAATTTATAGAAGTAT
GCAGGGAAATGTTTGAGAGTAAAGAAATTAAAAAAATTACCTATGATGCAAAAAATCCAAGGACTATACTAAGAAAATTA
GGAATAGAGTTTAATGGTATAAAATTTGATATAAATTTAGCTGCATATCTAATAAATCCAGTGAGGAAAGAATATGAGAT
ATCTAGTTTAGCTTCGGAATATTTGTTTAAAAATATAAATAGAGAAGATGAATTTTTAAAAATAAAAGAAGTTAATATAA
TGCCTAATTTATATAAAGTGCTGGAAGAAAAAATAAAAGAGCAAGATATGGAAGAACTGTTATATAAGGTAGAACAACCT
CTTACAGAAACATTATCAGCTATGGAGACAGAAGGTTTTAAAGTTGATAAAGATATACTTTTACAGCTAGAGAAAAAATT
TAAAGGGGAAATAGAAAGTACTCAGCTAGAAATATATTCAATGTCTGAAGAAGAATTTAATATAAATTCTCCTAAACAGT
TAGGGAAAATACTCTTTGAAAAATTAGATTTACCCGTTATAAAGAAAACAAAGACAGGATATTCTACTAATGCAGAGGTA
CTGGATAAATTAAGAGATAAACATCCAATAATAGATAAAATAACTTATTATAGACAATTAACAAAAATATACTCTACTTA
TATAGAAGGGCTAAAAGCTGCTATAGATGAGGATGGGAAAATACATTCAAATTTTAATCAAACTGTTACAGCTACAGGGA
GATTATCTAGTACAGAGCCTAATCTTCAAAATATACCTATAAAATATGAAATGGGAAGAGAAATAAGAAAGGTATTTATC
CCTAATACAGGGGATTCTGTAATACTTTCAGCGGATTATTCACAAATAGAATTAAGAGTATTAGCACATATATCTGATGA
TAAAAATATGATAAGTGCCTTTAATGAACATGATGATATTCATACTAAAACTGCATCAGAAGTGTTTAAAGTTCCAATAG
AAGAAGTAACACCTCTTATGAGAAGTAACGCTAAAGCAGTTAATTTTGGTATAGTTTATGGTATAGGAGACTTTAGCTTA
TCTCAAGATTTAAATATAACTAGAAAAGAAGCAAAGCAGTATATAGATGCTTATTTGGAGAGATATCCTAATGTAAAACT
TTATTTAGAAAATATAGTAGAAGAAGCCGTTGAAAAAGGGTATGTAAGTACTATATTAAATAGAAGAAGATATATAAAAG
AGGTTAAGTCTTCAAATAAAATAGTTAAAGCTGCAGGAGAAAGGCTAGCTATGAATAGTCCAATACAAGGAAGTGCAGCA
GATATAATAAAATTAGCTATGGTAAATGTTCATAGAAAATTAAAAGAAGATAATTTTAAAAGTAGTATAATATTACAAGT
ACATGATGAATTAATATTAAATGTCCATAAAGATGAACTAGAAAAAATCAAAGCTTTAGTAAAAAAAGAAATGGAACAGG
TTTTAGAATTAAAAGTTGGGTTAGACGTGGATATAAACATAGGTAATAATTGGTATGAAGCAAAGTAG

Protein sequence :
MNKERLLILDSNSLLNRAFYALPDLMTGEGIHTNAIYGFVNMLLKMKEEINPDYIVAAFDRKAPTFRHEEYKDYKAGRKK
MPEELAQQFPIIKELLSALAINIFEIDGFEADDLIGTLSVFAEEKGIEVYIVTGDKDALQLATDSVKVVITKKGITEKEI
YDKNRMVEEFGVTPKEFIDVKGLMGDTSDNIPGVPGIGEKTAFKLIKEYKSIETVFENIENIKGKKLKENLHEYREQAIF
SKKLATIICNVPIDMSLEDIKSKEEYDINKVREMFERLEFKSLMNKVGKGNEGETVEEEVSYKNIVSLEEFRDLKNNIIK
YKENDLYLYFELEDIVLFSKSRVKTLYANFKDEVYRIDFEMLLNNTKEQFIEVCREMFESKEIKKITYDAKNPRTILRKL
GIEFNGIKFDINLAAYLINPVRKEYEISSLASEYLFKNINREDEFLKIKEVNIMPNLYKVLEEKIKEQDMEELLYKVEQP
LTETLSAMETEGFKVDKDILLQLEKKFKGEIESTQLEIYSMSEEEFNINSPKQLGKILFEKLDLPVIKKTKTGYSTNAEV
LDKLRDKHPIIDKITYYRQLTKIYSTYIEGLKAAIDEDGKIHSNFNQTVTATGRLSSTEPNLQNIPIKYEMGREIRKVFI
PNTGDSVILSADYSQIELRVLAHISDDKNMISAFNEHDDIHTKTASEVFKVPIEEVTPLMRSNAKAVNFGIVYGIGDFSL
SQDLNITRKEAKQYIDAYLERYPNVKLYLENIVEEAVEKGYVSTILNRRRYIKEVKSSNKIVKAAGERLAMNSPIQGSAA
DIIKLAMVNVHRKLKEDNFKSSIILQVHDELILNVHKDELEKIKALVKKEMEQVLELKVGLDVDINIGNNWYEAK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
polA YP_281523.1 DNA polymerase I Not tested Not named Protein 3e-169 45