Gene Information

Name : YBT020_12035 (YBT020_12035)
Accession : YP_005566063.1
Strain : Bacillus thuringiensis YBT-020
Genome accession: NC_017200
Putative virulence/resistance : Virulence
Product : 2,3-dihydroxybenzoate-AMP ligase
Function : -
COG functional category : -
COG ID : -
EC number : -
Position : 2375820 - 2377436 bp
Length : 1617 bp
Strand : +
Note : COG1021 Peptide arylation enzymes

DNA sequence :
ATGCTAACAGGTTATACAAAATGGCCAAAAGAATTTGCTGATCGTTATCGAGAGGAAGGGTGTTGGCTTGGGGAAACATT
TGGCGGGATGTTAAGAGAGCGAGCTGAAAAATATGGCGATCAAATTGCAGTTGTAAGTGGTAATACGCATATTACGTATA
GTGAGTTTGATAAAAAAGTAGATCGATTAGCTTCAGGGCTACTGAATTTAGGAATAAAGCAAGAGGATCGAGTTGTAATC
CAGCTACCTAACGTTATTGAGTTTTTCGAAGTATGTTTTGCGCTATTTCGAATTGGGGCACTTCCTGTTTTTGCATTACC
TTCACATCGAAGCAGTGAAATTAGTTATTTTTGTGAGTTTGGTGAGGCGAGTGCTTACGTTATTTCAGATAAAGCTCTCG
GTTTTGATTATCGAAAACTAGCAAGAGAAGTGAAAGAGAAAGTGCCAACTTTACAACATGTAATTGTAGTGGGAGAAGAA
GAAGAATTTGTGAACATAAGTGATTTATATATAGATCCCGTTCCATTACCAGAAGTTCAGCCAAGTGATGTTGCGTTTCT
CCAATTATCAGGGGGGACAACAGGACTTTCTAAATTAATTCCTCGAACGCATGATGATTATATTTATAGTTTACGTGTTA
GCGCTGAAATTTGTAATTTAAGTGCAAGGAGTGTCTATATGGCCGTTCTTCCAGTAGCACATAATTACCCGATGAGCTCT
CCTGGAACATTTGGAACTTTCTATGCGGGTGGAAAAGTAGTGCTGGCAACTGGAGGTAGTCCAGATGAGGCATTTGCACT
TATTGAAAAAGAAAAAGTTACGATTACTGCTCTCGTACCGCCATTAGCAATGATATGGCTTGATGCTGCATCTTCACGTA
ATAACGATTTATCGAGTCTAGAAGTGATTCAAGTAGGTGGCGCGAAATTTAGTGCTGAAGTTGCAAAACGTATACGCCCT
ACATTTGGATGTACATTACAGCAAGTATTCGGTATGGCGGAAGGATTAGTGAACTATACAAGATTAGATGATCCTGAAGA
AATTATTATTCATACACAAGGTAGACCAATGTCTGCACTCGATGAAGTACGAGTTGTTGATGAAAATGACAACGATGTAA
AACCTGGCGAAGTAGGTAGTTTATTAACAAGAGGGCCATATACAATTCGTGGTTATTATAAAGCAGAAGAGCATAATGCA
CAATCGTTTACAAAGGATGGATTTTATCGCACAGGAGATCTTGTGAAAGTAAATGAACAAGGTTACATCATTGTAGAAGG
AAGAGATAAAGATCAAATTAATCGTGGTGGTGAGAAAGTTGCCGCGGAAGAAGTTGAAAATCATTTATTAGCACATGATG
CAGTGCATGATGTAGCAATTGTATCGATGCCTGATGACTATTTAGGAGAACGTACTTGTGCATTTGTTATAGCTCGCGGA
CAAGCTCCGGCAGTAAGTGAATTAAAAAAATTTTTAAGAGAGCGTGGTATAGCAGCTTATAAGATTCCAGATCGAATTGA
ATTTATTGAATCATTCCCGCAAACAGGCGTTGGAAAGGTAAGCAAAAAAGAATTACGTAAAGTCATTGCTGAAAAACTTA
TTACAGTAAAACAATAA

Protein sequence :
MLTGYTKWPKEFADRYREEGCWLGETFGGMLRERAEKYGDQIAVVSGNTHITYSEFDKKVDRLASGLLNLGIKQEDRVVI
QLPNVIEFFEVCFALFRIGALPVFALPSHRSSEISYFCEFGEASAYVISDKALGFDYRKLAREVKEKVPTLQHVIVVGEE
EEFVNISDLYIDPVPLPEVQPSDVAFLQLSGGTTGLSKLIPRTHDDYIYSLRVSAEICNLSARSVYMAVLPVAHNYPMSS
PGTFGTFYAGGKVVLATGGSPDEAFALIEKEKVTITALVPPLAMIWLDAASSRNNDLSSLEVIQVGGAKFSAEVAKRIRP
TFGCTLQQVFGMAEGLVNYTRLDDPEEIIIHTQGRPMSALDEVRVVDENDNDVKPGEVGSLLTRGPYTIRGYYKAEEHNA
QSFTKDGFYRTGDLVKVNEQGYIIVEGRDKDQINRGGEKVAAEEVENHLLAHDAVHDVAIVSMPDDYLGERTCAFVIARG
QAPAVSELKKFLRERGIAAYKIPDRIEFIESFPQTGVGKVSKKELRKVIAEKLITVKQ

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 6e-120 50
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 6e-120 50
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 6e-120 50
ybtE CAA21394.1 - Virulence HPI Protein 6e-120 50
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 1e-120 50
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 5e-120 50
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 1e-120 50
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 8e-120 50
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 6e-120 50

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
YBT020_12035 YP_005566063.1 2,3-dihydroxybenzoate-AMP ligase VFG0168 Protein 3e-136 52
YBT020_12035 YP_005566063.1 2,3-dihydroxybenzoate-AMP ligase VFG0932 Protein 3e-112 47
YBT020_12035 YP_005566063.1 2,3-dihydroxybenzoate-AMP ligase VFG1817 Protein 8e-111 44