Gene Information

Name : I33_3289 (I33_3289)
Accession : YP_005558179.1
Strain : Bacillus subtilis RO-NN-1
Genome accession: NC_017195
Putative virulence/resistance : Virulence
Product : 2,3-dihydroxybenzoate-AMP ligase
Function : -
COG functional category : -
COG ID : -
EC number : 2.7.7.58
Position : 3079439 - 3081058 bp
Length : 1620 bp
Strand : -
Note : -

DNA sequence : 
ATGCTGAAAGGATTTACGCCTTGGCCTGATGAGTTAGCTGAGACCTATCGAAAGAACGGCTGCTGGGCCGGTGAGACGTT
TGGAGATTTGTTAAGGGACCGGGCAGCCAAATATGGTGATCGCATCGCCATTACATGCGGGAACACCCATTGGAGCTATC
GAGAGCTTGACACAAGGGCTGATCGCCTGGCTGCCGGATTTCAAAAGCTCGGCATTCAACAGATGGATCGTGTCGTGGTG
CAGCTGCCAAATATCAAAGAGTTTTTTGAAGTTATTTTCGCGCTTTTCCGCCTGGGGGCGCTTCCAGTCTTCGCGCTGCC
TTCTCATCGAAGCAGTGAAATTACATATTTTTGCGAGTTTGCTGAAGCGGCTGCATATATCATTCCCGACGCTTATTCCG
GTTTTGACTACCGTTCGCTTGCCAGACAGGTTCAAAGCAAGCTGCCGACTTTGAAAAACATCATTGTCGCAGGTGAGGCA
GAGGAATTTTTGCCGCTGGAGGATTTGCATACAGAACCTGTAAAACTGCCTGAAGTCAAATCTTCGGATGTGGCGTTCCT
CCAGCTTTCTGGCGGGAGCACCGGGCTATCGAAACTGATTCCGAGGACCCACGATGACTACATATACAGCCTGAAGCGGA
GTGTGGAGGTCTGCTGGCTCGACCACAGCACGGTGTACTTGGCCGCGCTGCCGATGGCACACAATTATCCGCTGAGCTCA
CCTGGCGTTCTCGGCGTATTATACGCTGGAGGAAGAGTGGTATTATCTCCTTCTCCAAGTCCAGATGACGCATTTCCTTT
AATTGAACGGGAAAAGGTCACGATAACCGCTCTTGTTCCGCCTCTTGCGATGGTATGGATGGATGCGGCATCCTCACGCC
GTGATGATTTATCCAGCCTTCAAGTGCTGCAGGTCGGCGGTGCCAAGTTTAGTGCTGAAGCCGCGCGCAGGGTAAAAGCT
GTTTTCGGCTGCACGCTCCAGCAGGTGTTCGGAATGGCAGAGGGTCTCGTCAATTATACGAGATTGGATGATCCTGAGGA
GATCATTGTCAACACCCAAGGAAAACCGATGTCCCCATATGATGAAGTGCGTGTTTGGGATGATCATGATCGCGACGTAA
AACCTGGTGAAACAGGCCACTTGCTAACGCGGGGACCGTATACAATTCGAGGTTACTATAAGGCGGAAGAGCATAATGAC
GCTTCATTTACTGAGGATGGTTTTTACCGTACGGGTGATATCGTCAGGCTGACACCAGACGGCTATATTGTCGTTGAAGG
CCGGGCGAAGGATCAAATTAACCGTGGAGGAGAAAAGGTTGCGGCTGAAGAGGTAGAAAATCATCTGCTGGCGCATCCGG
CTGTCCATGATGCGGCAATGGTCTCCATGCCTGATCAATTTCTTGGCGAAAGATCTTGTGTGTTCATTATTCCCCGGGAT
GAAGCCCCAAAAGCCGCAGAGCTCAAAGCATTTTTGAGAGAGCGCGGACTGGCGGCATATAAAATCCCTGATCGAGTTGA
ATTTGTCGAATCCTTTCCGCAGACAGGGGTAGGAAAGGTAAGCAAAAAGGCTCTTCGTGAAGCTATTTCCGAGAAACTTC
TTGCAGGGTCAAAAAAATAA

Protein sequence : 
MLKGFTPWPDELAETYRKNGCWAGETFGDLLRDRAAKYGDRIAITCGNTHWSYRELDTRADRLAAGFQKLGIQQMDRVVV
QLPNIKEFFEVIFALFRLGALPVFALPSHRSSEITYFCEFAEAAAYIIPDAYSGFDYRSLARQVQSKLPTLKNIIVAGEA
EEFLPLEDLHTEPVKLPEVKSSDVAFLQLSGGSTGLSKLIPRTHDDYIYSLKRSVEVCWLDHSTVYLAALPMAHNYPLSS
PGVLGVLYAGGRVVLSPSPSPDDAFPLIEREKVTITALVPPLAMVWMDAASSRRDDLSSLQVLQVGGAKFSAEAARRVKA
VFGCTLQQVFGMAEGLVNYTRLDDPEEIIVNTQGKPMSPYDEVRVWDDHDRDVKPGETGHLLTRGPYTIRGYYKAEEHND
ASFTEDGFYRTGDIVRLTPDGYIVVEGRAKDQINRGGEKVAAEEVENHLLAHPAVHDAAMVSMPDQFLGERSCVFIIPRD
EAPKAAELKAFLRERGLAAYKIPDRVEFVESFPQTGVGKVSKKALREAISEKLLAGSKK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 3e-116 49
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 3e-116 49
ybtE CAA21394.1 - Virulence HPI Protein 3e-116 49
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 3e-116 49
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 2e-116 49
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 4e-116 49
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 3e-116 49
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 3e-116 49
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 3e-116 49

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
I33_3289 YP_005558179.1 2,3-dihydroxybenzoate-AMP ligase VFG0168 Protein 2e-134 54
I33_3289 YP_005558179.1 2,3-dihydroxybenzoate-AMP ligase VFG0932 Protein 5e-111 49
I33_3289 YP_005558179.1 2,3-dihydroxybenzoate-AMP ligase VFG1817 Protein 7e-112 45