Gene Information

Name : Spiaf_2102 (Spiaf_2102)
Accession : YP_005475857.1
Strain : Spirochaeta africana DSM 8902
Genome accession: NC_017098
Putative virulence/resistance : Virulence
Product : FliI/YscN family ATPase
Function : -
COG functional category : -
COG ID : -
EC number : -
Position : 2387056 - 2388438 bp
Length : 1383 bp
Strand : -
Note : PFAM: ATP synthase alpha/beta family, beta-barrel domain; ATP synthase alpha/beta family, nucleotide-binding domain; TIGRFAM: ATPase FliI/YscN family

DNA sequence :
ATGTCGATGTTCGATAAATATGCACAGGTTATCGAGACAATTGAACCGATGCGCTACAGCGGCGAGGTGGTATCCATCAA
GGGACTGCTGATCGAGAGCCACGGCCCGCAGGCGGTTCTGGGTGAGGTGTGTACCATCGAGATCCCGCGCACCGGCAAGA
CGGTGTATGCCGAGGTTATCGGTATACAGGATACGATAATTCAGCTGATGCCGTATCAGGAGCCTGAGGGAATAGAGCTG
GGATGCCGCGTTGTCGCAACCGGACAGCCGCTGCAGGCCCCGGTATCACTGAACATGCTTGGCAGGGTAATCGATGCCAT
GGGGCGTCCTGTTGACGGCAAGGGGCCGCTGCAGATAGATGGTACCTATCCGGTATTCAATACCCCTCCCGAAGCCCTGC
AGCGCAGTATGATCAGCGAGCAGCTGCAGACCGGGGTACGGGTAATTGACGGACTGAGCGCGGTCGGTAAGGGACAGCGT
CTGGGAATTTTTGCCGGCAGCGGGGTAGGGAAATCCACCATAATCAGTATGGTGGCACGCAATACGAATGCCGACATCAA
TGTGATTGCACTTATCGGCGAGCGTGGACGCGAGGTGCGTGAATTTATCGAGAACGACCTGGGTGAAGAAGGGTTGGCCC
GTTCCATCGTGGTGGTCTCGACCTCCGATACACCGGCCCTGGCGCGACTGCGGGGTGGTTATGTGGCGACTGCTATTGCC
GAGTATTTTCGGGATCAGGGGAATGATGTATTACTGCTGTTCGACTCGGTTACCCGTTTTGCCATGGCTCAACGTGAAAT
TGGCCTGACACGTGGAGAGGCGCCAGCCAGTCGGGGGTTTACCCCCTCAATGTTTGCCGAGCTGCCGCGTCTGCTGGAGC
GAACCGGTACCTCGGATCGTGGCAGCATCACCGGTTTCTACTCGATTCTTGTCGAGGGTGATGACATGAACGAGCCGGTG
TCCGATACCGTGCGGGGTATACTCGACGGCCATATTGTCCTGAGTCGTTCGCTGGCCGAAAAATATCACTATCCCGCGGT
TGATGCCCTGGCCAGCCTGTCGCGTCTGGCGAACAAGGTAACCACTGCACAGATGCAGAAAGCGGCCGGTCAGATTCGTC
GATTGCTGGCAGTATATCGTGATGCCGAAGACCTGATACACGTGGGCGCCTATCATCATGGAAGCAATCCGGATATAGAT
GCCGCCATCGAACTGTTGCCCAAAATCAATGCCTTTCTGCGGCAGGGGGTGATGGAGCGCAGCAGTCTGCCCGAGGTGAT
GTACGCCATGCAGGAGATTACCGGAATCGAGATCCCTGGTGCAGATGCCGGAAGCGATCAGCGCGACAAGGCGCAGACAG
AAACGGAGCCGGGACGTGCGTAG

Protein sequence :
MSMFDKYAQVIETIEPMRYSGEVVSIKGLLIESHGPQAVLGEVCTIEIPRTGKTVYAEVIGIQDTIIQLMPYQEPEGIEL
GCRVVATGQPLQAPVSLNMLGRVIDAMGRPVDGKGPLQIDGTYPVFNTPPEALQRSMISEQLQTGVRVIDGLSAVGKGQR
LGIFAGSGVGKSTIISMVARNTNADINVIALIGERGREVREFIENDLGEEGLARSIVVVSTSDTPALARLRGGYVATAIA
EYFRDQGNDVLLLFDSVTRFAMAQREIGLTRGEAPASRGFTPSMFAELPRLLERTGTSDRGSITGFYSILVEGDDMNEPV
SDTVRGILDGHIVLSRSLAEKYHYPAVDALASLSRLANKVTTAQMQKAAGQIRRLLAVYRDAEDLIHVGAYHHGSNPDID
AAIELLPKINAFLRQGVMERSSLPEVMYAMQEITGIEIPGADAGSDQRDKAQTETEPGRA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 4e-98 52
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 6e-99 52
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 6e-99 52
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 4e-99 52
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 5e-98 52
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 9e-99 52
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 9e-99 52
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 8e-99 52
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 7e-99 52
lscN AAO18044.1 LscN Virulence TTSS locus Protein 6e-96 51
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 4e-90 50
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 4e-88 49
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 3e-88 49
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 1e-87 49
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 1e-87 49
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-88 48
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 2e-78 45
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 2e-78 45
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 3e-76 44
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 2e-84 44
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 2e-77 44
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 2e-76 44
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 1e-76 44
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 8e-77 44
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 5e-78 44
escN CAI43874.1 EscN protein Virulence LEE Protein 3e-83 44
escN CAC81862.1 EscN protein Virulence LEE II Protein 2e-83 44
escN YP_003223475.1 translocator EscN Virulence LEE Protein 3e-83 44
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 3e-83 44
escN YP_003236088.1 translocator EscN Virulence LEE Protein 2e-83 44
escN AAK26715.1 EscN Virulence LEE Protein 5e-83 44
escN AAL57542.1 EscN Virulence LEE Protein 5e-83 44
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 1e-71 43
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-81 43
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-81 43
escN AAC38383.1 EscN Virulence LEE Protein 6e-84 42
escN AAC31513.1 L0034 Virulence LEE Protein 2e-84 42
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 2e-84 42
escN NP_290268.1 hypothetical protein Virulence LEE Protein 3e-84 42
ECs4568 NP_312595.1 EscN Virulence LEE Protein 3e-84 42
unnamed AAL06369.1 EscN Virulence LEE Protein 4e-82 42
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 4e-68 42
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 1e-72 42
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 1e-72 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Spiaf_2102 YP_005475857.1 FliI/YscN family ATPase VFG2144 Protein 2e-106 53
Spiaf_2102 YP_005475857.1 FliI/YscN family ATPase VFG0192 Protein 5e-99 50
Spiaf_2102 YP_005475857.1 FliI/YscN family ATPase VFG0048 Protein 9e-99 49
Spiaf_2102 YP_005475857.1 FliI/YscN family ATPase VFG0390 Protein 2e-95 49
Spiaf_2102 YP_005475857.1 FliI/YscN family ATPase VFG0515 Protein 2e-88 49
Spiaf_2102 YP_005475857.1 FliI/YscN family ATPase VFG1254 Protein 3e-90 48
Spiaf_2102 YP_005475857.1 FliI/YscN family ATPase VFG2499 Protein 3e-89 48
Spiaf_2102 YP_005475857.1 FliI/YscN family ATPase VFG2331 Protein 1e-82 47
Spiaf_2102 YP_005475857.1 FliI/YscN family ATPase VFG0812 Protein 1e-84 42
Spiaf_2102 YP_005475857.1 FliI/YscN family ATPase VFG0730 Protein 3e-84 42
Spiaf_2102 YP_005475857.1 FliI/YscN family ATPase VFG2459 Protein 9e-67 41