Gene Information

Name : Spiaf_2001 (Spiaf_2001)
Accession : YP_005475758.1
Strain : Spirochaeta africana DSM 8902
Genome accession: NC_017098
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : -
COG ID : -
EC number : -
Position : 2275867 - 2277645 bp
Length : 1779 bp
Strand : +
Note : PFAM: Pyruvate kinase, barrel domain; Pyruvate kinase, alpha/beta domain; TIGRFAM: pyruvate kinase

DNA sequence :
ATGATGATGAACACATTACGCAAGACCCGCATCGTTTGTACCATCGGCCCCGCCGTTGATGATGTGGAGACTATCAAAGG
CATGCTCAAGGCCGGCATGAATGTTGCCCGGTTCAATTTTTCGCATGACTACCATGAAATCCACGCCCAGCGCATCGAAA
AGGTGCGCCAGGCAAGCCGCGAAACCAAAATCCCGGTAGCTATCCTGCTGGACACCAAGGGACCGGAGATTCGCACCGGC
CAGATCAAGGATGGCGGTAAAATCAATCTGGAGAACGGAAAAAAGATCACCCTGACCACCGAACAGGTAGAGGGCACTGC
CGAGCTCCTGAGCATCAGCTATCAGAAACTCCCCGAAGAGGTTACCCCCGGCACTCATATCCTTGTTGCCGACGGCGTAG
TCGACCTTGAGGTTGAATCGGTAGACGGCAACAAGATCCATTGTCTGATCCGCGCGGGCGGACAGCTGGGCAGCAAGAAA
AACGTGAATGTTCCCGGCATCAAGACCAGCCTGCCGGCCATCACCGAGAAGGATCGCGACGACATCGTATTCGGCGCCAA
GAACAACGTTGACTTCATCGCTGCATCCTTTATCCGCAAGGCCGCCGATGTACAGGAGATACTCGATCTGCTGGCTGAAC
ATGACTCTCAGACCCGTGTCATCTCCAAGATCGAGGATCAGGAAGGCCTGGACAACATCGATGACATCATCAATGTATCT
CATGGCATCATGATTGCGCGTGGCGACCTCGGGGTGCAGCTGCCGCCGGAAAAAATTCCCATGGCACAGAAACGCATCAC
CGAGAAGTGCAACCGCCAGAACAAGCCGGTTATTACCGCTACCCAGATGCTCGACTCGATGATCAACAATCCCAGCCCTA
CCCGTGCAGAGCTTACCGACGTTGCCAATGCAATCTTTGATGGCACCGATGCTATCATGCTGAGCGGTGAAACTGCCAGC
GGCGATTATCCGATCAAGTCCGTGGAGACCATGCACAATGTTGCCATGGCAGTCGAAACCTCGCCCGAGTTTCTTGAACG
TACAGAGAAATATTTCGCTTTCCATGAGACAACCGGCGAAATTGGCCATGCAGTAGCAAAATCCGCATATCTGCTGGCAC
AGGCTGTCGATGCAACCGCGATTGTTACCCCTACCCTGCGCGGCAACACCCCGCGCCTGCTGAGCAAGTTCCGCCCGAAG
CAGAACGTAATTGCGGTAACCACCAGTGAAACCGCATATCGGCAGCTGCTGGTTCAGTGGGGGGTATTCCCGATTCTGGC
AGAGTTTGTAAACAACTCCGAGGTCATGATTCAGAACGCCCTGCGTGCTGCGCAGAAATCAGGCTTTATCCGCCGCAACG
ACAAAGTCGTAACCGCTGCCGGTCTGCCGCTGAATTCTCCGATCCCGATGAACACCATCAAGGTGCATTTTATGGGCAAC
ATTCTGAACCGAGGACAGGACGGCGCCGGGGCCAAAACAGCCGGCCTGCTGATCAAGGGAGAAACCCTGAGCGACGCTGC
AGCCAAGGCCCCCAAAGACACCTCGTACGTGCTGCTTGCCAAGACTATCAGCAGCAGCGACAAGCTTGAGCTGCTGAAAA
ATGCTGCCGGCCTGATCGTTGAGGAACCGCTGACTGATCTGAAGGCACTCACTGCCATAAACAGCGACCTGGTTGTGGTA
AGCGGTGTACCCAGTGCACACGCCCAGTTCGAGGAAGGTCTGATGGTCAGCCTCGACGGAGTCGAAAAGATCATCTACGA
GGGTGCTCTGGAGGACTAA

Protein sequence :
MMMNTLRKTRIVCTIGPAVDDVETIKGMLKAGMNVARFNFSHDYHEIHAQRIEKVRQASRETKIPVAILLDTKGPEIRTG
QIKDGGKINLENGKKITLTTEQVEGTAELLSISYQKLPEEVTPGTHILVADGVVDLEVESVDGNKIHCLIRAGGQLGSKK
NVNVPGIKTSLPAITEKDRDDIVFGAKNNVDFIAASFIRKAADVQEILDLLAEHDSQTRVISKIEDQEGLDNIDDIINVS
HGIMIARGDLGVQLPPEKIPMAQKRITEKCNRQNKPVITATQMLDSMINNPSPTRAELTDVANAIFDGTDAIMLSGETAS
GDYPIKSVETMHNVAMAVETSPEFLERTEKYFAFHETTGEIGHAVAKSAYLLAQAVDATAIVTPTLRGNTPRLLSKFRPK
QNVIAVTTSETAYRQLLVQWGVFPILAEFVNNSEVMIQNALRAAQKSGFIRRNDKVVTAAGLPLNSPIPMNTIKVHFMGN
ILNRGQDGAGAKTAGLLIKGETLSDAAAKAPKDTSYVLLAKTISSSDKLELLKNAAGLIVEEPLTDLKALTAINSDLVVV
SGVPSAHAQFEEGLMVSLDGVEKIIYEGALED

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 8e-90 44
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 3e-90 44
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 3e-90 44
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 7e-90 44

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Spiaf_2001 YP_005475758.1 pyruvate kinase VFG0479 Protein 1e-90 44