Gene Information

Name : SGRA_4128 (SGRA_4128)
Accession : YP_005324427.1
Strain : Saprospira grandis Lewin
Genome accession: NC_016940
Putative virulence/resistance : Virulence
Product : restriction enzyme type I helicase subunit; type I restriction enzyme HsdR
Function : -
COG functional category : -
COG ID : -
EC number : -
Position : 4251007 - 4254117 bp
Length : 3111 bp
Strand : -
Note : COG0610 Type I site-specific restriction-modification system, R (restriction) subunit and related helicases

DNA sequence :
ATGCCAAGCAAGACGAATGAACAGGCCTTAGAGGCGGCCATAGAAAAAGCCTTAACGGGTAGTAATTTAGAGGAGCTGAA
AGCGCAGGGTTATGCGCCCCAGCAGATGGCGGAGGGGGAGATCCACTACCGTTCGGGCAATGGGTTTTATATAGGGGACC
CTAATGATTATGATGCAAAATGGGCCATAGACAAGCGGCGTTTTTGGCATTTCTTAGAAAGCAGTCAGGCCGAGGAGTTG
GCCAAGCTGCGCAAGCGGTCGGATGGGGAATTAAAGATCCTTCATCGCTTGAATGTGCTCATCAAGAAATATGGGGTTTT
AGAACTGTTGCGCAAAGGGCTAGATGTTGATGATGCGCATTTTGAGTTATTGTATCCCTTGCCCTTAGCGAGTAGTGGGA
AAAGAGTTCGGCAGCAGTTTGAGCAAAATGAGTTTAGTGTGAGTCGGCAATTGCGTTATTCACTAGATAAGCCCTTACAG
GAAATAGACATGGTCATTTTTGTAAATGGTCTGCCTATAATTACGATGGAGCTCAAGAATCCTTGGACGGGGCAGAATGC
TCGTTATCATGGGCAAAAACAGTATAGAGAAGACCGAGATATACGGCAGCCGCTTTTGCAATTTGGTCGTTGTTTGGTTC
ATTTTGCGGTCGATACAGATGAGGTCTATATGACTACTCGTTTGGCGGGTAAAGGGACCTTCTTTTTGCCTTTTAATAAG
GGGAATGGGCAAAGCAAGGGGAATCCCGTTAATGAGTTTGGGCATAAAACCGCCTATTTGTGGGAGCAAATTTTCAAGCG
GGAGAGTTTGGCCAATTTGATTCAGCATTTTGTGCGCTTTGATGGCAAGCGAACGACTGCATTAAACAAGCGGACCTTAT
ATTTTCCTCGTTTTCATCAGTTGGATGTGGTTCGTAAGATTGTGGCGGATATAGAAGCGGAAGGCCTGGGAAAAACCTAT
TTGATTCAGCATTCGGCGGGTTCGGGAAAATCCAACTCTATAACTTGGGCGGCCTATCAGTTGATAGAGACCTACCCTAA
GCATGAAAATGTTAGGGGATTTAGAGGAATGGAAAGGCCTTTATTTGATTCGGTTATTGTGGTAACGGATCGGCGTTTAT
TAGATAAGCAAATTAGGGAGAACATTAAGAGTTTTTCTCAGGTCAAAAACATTGTTGCTCCAGCTTATCGCTCTGCAGAT
TTGAAGGCGGCATTAGAGCTGGGGAAAAAGATTATCATTACGACCATACAAAAGTTTCCTTTTGTTTTGGAGGGAATTAG
TCAGATGAATGAGAAGCAGTTTGCGGTAATTATAGATGAGGCGCATAGTTCACAGACGGGGACCACTGCAGACAAAATGA
ATATGACTTTTGCCAAAACAGATGATAGCTTGGGCGATGCTCAGGACAAGCTATTTGATTTGATGTCTTCCCGTAAGATG
CGGCAGAATGTTTCTTATTTAGCCTTTACGGCTACGCCAAAGAACTCGACCCTAGAAAAGTTTGGGGTTCGGCAGGCAGA
TGGGAGTTTTAAGCCTTTTCATTTGTATTCTATGAAGCAGGCCATAGAGGAGGGCTTTATTTTAGATGTTTTGGCCAATT
ACACGACCTACAAGAGCTACTATGAGCTACAAAAATCTATAGCGGAGAATCCCCTTTTTGATACCAAAAAGGCGCAAAAG
AAATTGCGGAAGTATGTAGAGCGGCAAGAGCGGACAATAGCGACCAAGGCCGAGATTATGCTCAATCATTTTATGGAGCA
AGTGCAGGCGAAGCGGAAGTTAAAGGGGAAAGGCAAGGCGATGGTCTTAACGCAGAGCATTGAGGCGGCTATTTTATATT
ATCAGGCCATGCAGCGATTGTTGGAGCAAAAGGGGAACCCCTTCAGGATATTGGTTGCATTTTCTGGTAAAAAAATGTTG
AAGGGTATAGAGTATACAGAAGAGCAGATCAACTATTTACCTGCCGAATTAGATCGGGCAAAGTCTTCGGACCCAGACTA
CATCAGTGATAAGATTGCCCGTTATTTTGATATGGATGAATATCGGATATTGATTGTGGCCAACAAGTACCTTACAGGTT
TTGATCAGCCCAAGCTTTGTAGTATGTATATTGATAAAAAGCTACAAGGGGTTTTGGCGGTACAGGCTTTATCGCGTCTG
AATCGCTCGGCTAATAAATTGGGCAAGAAGACCGAAGATCTTTTTGTATTGGACTTCTTTAATAGTACAGAAGACATCAA
AAAAGCCTTTGATGATTTTTATACCGCAACCAGTTTGTCTGAGGCTACAGATGTGAATATATTACATGAGTTAAAGGACC
ATCTAGAAGACCCTGGGGTATATGAACTGGCCGAAGTAAAGGAGTTTACTAATTTATTCTTTGATAATGCTGATGCCCAG
CTATTGAGTCCAATTATAGACCAGGCAGCTCATCGTTTTAATGAGGAGCTAGAACTTACCGATGAAGAAAAAGCAGACTA
TAAAATTAAGGCCAAGCAGTTTGTTAAGATTTATGGGCAACTGGCTTCAATTATGGATATGGATATGGATTATAGCCGTT
TGAATTGGGAGCAGCTATTTTGGTTCTTAAAGTTTCTTATTCCCAAGATGGTCATTAGGAATAAAGATAAGGACCAGCTA
GACAGCTTATTGGATGCCGTAGATTTGTCTACTTATGGTATTGAGCGCGTAAAGCTCAATCATAAAATAGAATTGGATGC
AGAGGAAACTAGCCTAAAACCTCAGAATGCCAACCCCAGAGGAGCTCATGAAGAAGAGGAAAAAGATACTTTAGAAGAAA
TCTTGAGGATGTTTAATGAGCGCTGGTTTAAGGATTGGGGGGCTACCCCAGAGGAGCAGCGGATTCGTTTTGCCTCTTTT
GGAGAGGGCATTTTGAACCACCCAGATTATAAGGATAAGGTTATGGAAAATCCCGATGCACAGAATAGGGATATGGCTTT
CGATAAAATTATGAAAGAGGTCATGACGCATCATCGCAAACTGGATATTCAGCTCTATCGGAAGTTTGTACAAGATGAAG
GTTTTGCTCAAGCCATTATTGATGGGCTGAAGCGCTATGTGGAGCAAGAGGCGATTAAGCGGAATATTTAG

Protein sequence :
MPSKTNEQALEAAIEKALTGSNLEELKAQGYAPQQMAEGEIHYRSGNGFYIGDPNDYDAKWAIDKRRFWHFLESSQAEEL
AKLRKRSDGELKILHRLNVLIKKYGVLELLRKGLDVDDAHFELLYPLPLASSGKRVRQQFEQNEFSVSRQLRYSLDKPLQ
EIDMVIFVNGLPIITMELKNPWTGQNARYHGQKQYREDRDIRQPLLQFGRCLVHFAVDTDEVYMTTRLAGKGTFFLPFNK
GNGQSKGNPVNEFGHKTAYLWEQIFKRESLANLIQHFVRFDGKRTTALNKRTLYFPRFHQLDVVRKIVADIEAEGLGKTY
LIQHSAGSGKSNSITWAAYQLIETYPKHENVRGFRGMERPLFDSVIVVTDRRLLDKQIRENIKSFSQVKNIVAPAYRSAD
LKAALELGKKIIITTIQKFPFVLEGISQMNEKQFAVIIDEAHSSQTGTTADKMNMTFAKTDDSLGDAQDKLFDLMSSRKM
RQNVSYLAFTATPKNSTLEKFGVRQADGSFKPFHLYSMKQAIEEGFILDVLANYTTYKSYYELQKSIAENPLFDTKKAQK
KLRKYVERQERTIATKAEIMLNHFMEQVQAKRKLKGKGKAMVLTQSIEAAILYYQAMQRLLEQKGNPFRILVAFSGKKML
KGIEYTEEQINYLPAELDRAKSSDPDYISDKIARYFDMDEYRILIVANKYLTGFDQPKLCSMYIDKKLQGVLAVQALSRL
NRSANKLGKKTEDLFVLDFFNSTEDIKKAFDDFYTATSLSEATDVNILHELKDHLEDPGVYELAEVKEFTNLFFDNADAQ
LLSPIIDQAAHRFNEELELTDEEKADYKIKAKQFVKIYGQLASIMDMDMDYSRLNWEQLFWFLKFLIPKMVIRNKDKDQL
DSLLDAVDLSTYGIERVKLNHKIELDAEETSLKPQNANPRGAHEEEEKDTLEEILRMFNERWFKDWGATPEEQRIRFASF
GEGILNHPDYKDKVMENPDAQNRDMAFDKIMKEVMTHHRKLDIQLYRKFVQDEGFAQAIIDGLKRYVEQEAIKRNI

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
VC1765 NP_231400.1 type I restriction enzyme HsdR Not tested VPI-2 Protein 0.0 62
VC0395_A1363 YP_001217306.1 type I restriction enzyme HsdR Not tested VPI-2 Protein 0.0 62
VPI2_0013c ACA01830.1 type I restriction enzyme HsdR Not tested VPI-2 Protein 0.0 62

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
SGRA_4128 YP_005324427.1 restriction enzyme type I helicase subunit; type I restriction enzyme HsdR VFG1098 Protein 0.0 62