Gene Information

Name : hrcN (PSF113_5606)
Accession : YP_005210973.1
Strain : Pseudomonas fluorescens F113
Genome accession: NC_016830
Putative virulence/resistance : Virulence
Product : protein HrcN
Function : -
COG functional category : -
COG ID : -
EC number : -
Position : 6468767 - 6470125 bp
Length : 1359 bp
Strand : -
Note : Type III secretion cytoplasmic ATP synthase (YscN,SpaL,MxiB,HrcN,EscN)

DNA sequence :
ATGACGCAGGCGTCCATCGCGCGACTGGAGGCCTGGGAGGCCGGGCAATTGGCGCACTTGCGTCGCTTCGCACCGGTCGT
GGTGCGCGGGCGGGTGCAGCGGGTCAGCGGCATCTTGCTGCAGTGCCGACTGCCGGGGGCGCAGATCGGCGACCTCTGCC
GGGTCGAGCGCGACGCCCGCGAGCCGCTGCTCGCCGAAATCGTCGGCTTCGACCAGCACGATGCCTTGCTCAGCGCCTTG
GGCGGGCTGGAGGGCGTCCGGGTCGGTGCGGCGGTGGAGCCGCTGGGCATGCCGCATCGGGTGTGCGTGGGGCCGCAGTT
GCTGGGCCAGGTGCTGGATGGCTTCGGCCGGCCACTGGTGGACGGCGGCGCGGGAGCCTTCGCCGGTGCGCATGTGCCGA
ATGCATCGCAGGTGATTGGCGAGGCCCCGGCGCCCACCGCTCGACCGCGCATCCAACGGCCCTTGATGACCGGCGTCCGG
GCCATCGACGGCCCGCTGACGCTCGGCGAAGGCCAGCGTGTGGGGCTGTTCGCCGGCGCCGGTTGTGGCAAGACCACGCT
GCTGGCGGAGATTGCCCGGAACGTTGACTGCGATGTCATCGTGTTCGGCCTGATCGGCGAGCGTGGGCGCGAACTGCGCG
AATTCCTCGACCATGAGCTGGATGATGAACTGCGTGCCCGTGCGGTGCTGGTGTGCGCCACCTCCGACCGCTCCAGCATG
GAACGCGCCCGTGCGGCGTTCACCGCGACGGCATTGGCCGAGGGGTTTCGTGCCCGGGGCCTGCGCGTTCTGCTGCTCAT
CGACTCGCTGACCCGCTTCGCCCGGGCCCAGCGTGAGATCGGCCTGGCCGCCGGCGAACCGTTGGGGCGCGGCGGCCTGC
CGCCGTCGGTCTACAGCCTGCTGCCGCGCCTGGTGGAGCGGGCCGGGCTGACCAACGAGGGCGCCATCACCGCGTTTTAT
ACGGTGCTCATCGAGCAGGACTCCATGAACGATCCCGTGGCGGATGAAGTGCGTTCGCTGCTCGACGGTCATATCGTGCT
GTCACGCAAGCTCGCGGAGCGCGGCCACTACCCGGCAATCGACGTGCTGGCCAGCCTGTCGCGCACCTTGGCCAACGTGG
CCGAGGCGGAGCACCTGCGGGCGGGCATCAATTTGCGTCGATTGCTCTCGGCCTATGAGCAAATCGAACTGATGTTGCGC
CTGGGCGAATACCAGGCCGGCGCCGACCCCTTGACCGACCTGGCGGTGGAGTCGCGTGGTGCCCTGGACGCCTTCCTGCG
CCAGGACCTGCGCGAGCCGGAGCCGTTTGCGTCCACGCTCGCCCACCTGCAGCAGTTGACCGCCTATGTCCCGGGTTGA

Protein sequence :
MTQASIARLEAWEAGQLAHLRRFAPVVVRGRVQRVSGILLQCRLPGAQIGDLCRVERDAREPLLAEIVGFDQHDALLSAL
GGLEGVRVGAAVEPLGMPHRVCVGPQLLGQVLDGFGRPLVDGGAGAFAGAHVPNASQVIGEAPAPTARPRIQRPLMTGVR
AIDGPLTLGEGQRVGLFAGAGCGKTTLLAEIARNVDCDVIVFGLIGERGRELREFLDHELDDELRARAVLVCATSDRSSM
ERARAAFTATALAEGFRARGLRVLLLIDSLTRFARAQREIGLAAGEPLGRGGLPPSVYSLLPRLVERAGLTNEGAITAFY
TVLIEQDSMNDPVADEVRSLLDGHIVLSRKLAERGHYPAIDVLASLSRTLANVAEAEHLRAGINLRRLLSAYEQIELMLR
LGEYQAGADPLTDLAVESRGALDAFLRQDLREPEPFASTLAHLQQLTAYVPG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 7e-132 72
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 4e-135 70
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 7e-126 70
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 6e-136 69
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 4e-136 69
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 5e-137 67
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 4e-134 67
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 1e-134 67
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 1e-134 67
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 9e-127 67
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 9e-127 67
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 2e-135 67
lscN AAO18044.1 LscN Virulence TTSS locus Protein 7e-95 55
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 2e-88 53
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 2e-82 53
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-82 53
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 6e-82 53
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 8e-82 53
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 3e-82 53
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-87 52
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 4e-87 52
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 8e-88 52
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 8e-88 52
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 8e-88 52
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 2e-88 52
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 6e-87 52
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-85 52
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-85 52
escN YP_003223475.1 translocator EscN Virulence LEE Protein 3e-78 47
escN AAC31513.1 L0034 Virulence LEE Protein 2e-77 47
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 2e-77 47
escN AAK26715.1 EscN Virulence LEE Protein 3e-78 47
escN NP_290268.1 hypothetical protein Virulence LEE Protein 3e-77 47
escN AAL57542.1 EscN Virulence LEE Protein 3e-78 47
ECs4568 NP_312595.1 EscN Virulence LEE Protein 3e-77 47
escN CAC81862.1 EscN protein Virulence LEE II Protein 2e-78 47
unnamed AAL06369.1 EscN Virulence LEE Protein 4e-76 47
escN YP_003236088.1 translocator EscN Virulence LEE Protein 9e-78 47
escN CAI43874.1 EscN protein Virulence LEE Protein 2e-78 47
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 4e-78 47
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-76 46
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-76 46
escN AAC38383.1 EscN Virulence LEE Protein 2e-77 46
invC AAS66861.1 InvC Not tested SSR-2 Protein 2e-66 44
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 6e-64 44
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 7e-66 44
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 9e-68 43
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 9e-68 43
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 9e-68 43
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 9e-68 43

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
hrcN YP_005210973.1 protein HrcN VFG0192 Protein 3e-95 56
hrcN YP_005210973.1 protein HrcN VFG0390 Protein 4e-93 55
hrcN YP_005210973.1 protein HrcN VFG0515 Protein 3e-82 53
hrcN YP_005210973.1 protein HrcN VFG0048 Protein 5e-91 52
hrcN YP_005210973.1 protein HrcN VFG1254 Protein 2e-73 49
hrcN YP_005210973.1 protein HrcN VFG2144 Protein 2e-85 48
hrcN YP_005210973.1 protein HrcN VFG0812 Protein 1e-77 47
hrcN YP_005210973.1 protein HrcN VFG0730 Protein 1e-77 46
hrcN YP_005210973.1 protein HrcN VFG1778 Protein 2e-67 45
hrcN YP_005210973.1 protein HrcN VFG2499 Protein 1e-67 45
hrcN YP_005210973.1 protein HrcN VFG2459 Protein 2e-70 44
hrcN YP_005210973.1 protein HrcN VFG0555 Protein 4e-68 43
hrcN YP_005210973.1 protein HrcN VFG2331 Protein 2e-69 43
hrcN YP_005210973.1 protein HrcN VFG1008 Protein 3e-65 42