Gene Information

Name : Clo1100_2030 (Clo1100_2030)
Accession : YP_005148027.1
Strain : Clostridium sp. BNL1100
Genome accession: NC_016791
Putative virulence/resistance : Resistance
Product : D-alanine--D-alanine ligase
Function : -
COG functional category : -
COG ID : -
EC number : -
Position : 2357442 - 2358491 bp
Length : 1050 bp
Strand : -
Note : PFAM: D-ala D-ala ligase C-terminus; D-ala D-ala ligase N-terminus; TIGRFAM: D-alanine--D-alanine ligase

DNA sequence :
ATGGATAGAAAAAACATTGCAATTATTTTCGGAGGTTGCTCGCCGGAATACGAAGTCTCACTTAATTCTGTTTCATCTGT
AATAGAACATGTCAACAATGACTTGTATAATACAATTTTAATAGGTATTACAAAAGAGGGCGAATGGCTCAGGTACTTCG
GAGATGTTGACAAAATCAGGAATAATACGTGGTTCCAGGAAAAAGGATGTAATCCTGTTGCTTTTTCCCAAAGCCGCAGC
ATACACGGTTTCTATGAATTTTCAGAGGGTTCAAGCTCCATTACTTTTGTTGATGCTGCCTTTCCCATACTTCATGGCAG
AAACGGTGAAGACGGTACTGTTCAGGGGATGCTGAAACTTGCAGGAATACCTTTTGTAGGCTGTGGTATTCTAAGTTCAG
CCATGTGCATGGACAAGGATATTGCTCATCGTATTGCACAATCGTCAGGAATACGTGTTCCAATATCGATATCAGCAGAT
AAAAACATGATTCTTAAAGATATTTTAATTATGGCTGAAACTCTCAGATACCCTGTTTTTGTCAAACCTGCAAGAGCTGG
TTCTTCTTTCGGTATAACACGTGCTAATAGCAGATCTGAGCTTGAAAAGGCAATTATAAATGCTTTTGAATACGACAGTA
AAATAGTTGTTGAAGAGAATATAGATGGTTTTGAGGTAGGTTGTGCCATCCTTGGAAACGATGATCTTATAATAGGTGAA
GTTGATGAGATTGAACTTCAGACCGGTTTTTTTGATTATAACGAAAAATATACATTAAAGACTTCCAAAATCCACATGCC
TGCCAGAATTGACAAAGTGACCTCAGAAAGGGTTAAAAATACTGCAAGGATTTTATACAATGCTCTGTACTGCACCGGAT
TCGCAAGGGTTGACATGTTTCTGACCCCAAAGGGAGAAATCGTATTTAACGAAGTCAATACCATACCCGGCTTTACAGCT
CACAGCCGTTATCCCAATATGCTCCGGGGAGCGGGCTTATCCTTTGCTTCCATAGTTGACAGGCTGATAAGGATGGCGAT
GGAAAAATGA

Protein sequence :
MDRKNIAIIFGGCSPEYEVSLNSVSSVIEHVNNDLYNTILIGITKEGEWLRYFGDVDKIRNNTWFQEKGCNPVAFSQSRS
IHGFYEFSEGSSSITFVDAAFPILHGRNGEDGTVQGMLKLAGIPFVGCGILSSAMCMDKDIAHRIAQSSGIRVPISISAD
KNMILKDILIMAETLRYPVFVKPARAGSSFGITRANSRSELEKAIINAFEYDSKIVVEENIDGFEVGCAILGNDDLIIGE
VDEIELQTGFFDYNEKYTLKTSKIHMPARIDKVTSERVKNTARILYNALYCTGFARVDMFLTPKGEIVFNEVNTIPGFTA
HSRYPNMLRGAGLSFASIVDRLIRMAMEK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
vanB NP_815951.1 D-alanine--D-lactate ligase Not tested Not named Protein 2e-66 46
vanB YP_008394259.1 Vancomycin B-type resistance protein vanB, D-alanine--D-lactate ligase Not tested Not named Protein 2e-65 44

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Clo1100_2030 YP_005148027.1 D-alanine--D-alanine ligase U00456.gene.p01 Protein 9e-67 46
Clo1100_2030 YP_005148027.1 D-alanine--D-alanine ligase U35369.1.gene6.p01 Protein 9e-67 46
Clo1100_2030 YP_005148027.1 D-alanine--D-alanine ligase AE016830.1.gene2250. Protein 9e-67 46
Clo1100_2030 YP_005148027.1 D-alanine--D-alanine ligase AF310956.2.orf5.gene Protein 9e-67 45
Clo1100_2030 YP_005148027.1 D-alanine--D-alanine ligase AY145441.gene.p01 Protein 6e-67 45
Clo1100_2030 YP_005148027.1 D-alanine--D-alanine ligase NC_005054.2598280.p0 Protein 4e-66 45
Clo1100_2030 YP_005148027.1 D-alanine--D-alanine ligase NC_014475.1.orf3.gen Protein 4e-66 45
Clo1100_2030 YP_005148027.1 D-alanine--D-alanine ligase AF277571.gene.p01 Protein 5e-65 45
Clo1100_2030 YP_005148027.1 D-alanine--D-alanine ligase AY489045.gene.p01 Protein 9e-64 44
Clo1100_2030 YP_005148027.1 D-alanine--D-alanine ligase AF130997.1.orf4.gene Protein 1e-62 43
Clo1100_2030 YP_005148027.1 D-alanine--D-alanine ligase L29638.gene.p01 Protein 4e-65 42
Clo1100_2030 YP_005148027.1 D-alanine--D-alanine ligase AF162694.gene.p01 Protein 2e-68 41