Gene Information

Name : BCN_2291 (BCN_2291)
Accession : YP_005104824.1
Strain : Bacillus cereus NC7401
Genome accession: NC_016771
Putative virulence/resistance : Virulence
Product : 2,3-dihydroxybenzoate-AMP ligase
Function : -
COG functional category : -
COG ID : -
EC number : -
Position : 2262161 - 2263777 bp
Length : 1617 bp
Strand : +
Note : similar to gpu:AE017271_301 [Bacillus cereus ATCC 10987], percent identity 97 in 538 aa, BLASTP E(): 0.0

DNA sequence : 
ATGCTAACAGGTTATACAAAATGGCCAAAAGAATTTGCAAATCGTTACCGAGAAGAAGGCTGTTGGCTTGGGGAAACATT
TGGCGGGATGTTAAGAGAGCGAGCTGAAAAATATGGCGATCAAATTGCAGTTGTAAGTGGTAATACGCATATTACGTATA
GTGAGTTTGATAAAAAAGTAGATCGATTAGCTTCAGGGCTACTGAATTTAGGAATAAAGCAAGAGGATCGAGTTGTAATC
CAGCTACCTAACGTTATTGAGTTTTTCGAAGTATGTTTTGCGCTATTTCGAATTGGGGCACTTCCTGTTTTTGCATTACC
TTCACATCGAAGCAGTGAAATTAGTTATTTTTGTGAGTTTGGTGAGGCGAGTGCTTACGTTATTTCAGATAAAGCTCTCG
GTTTTGATTATCGAAAACTAGCAAGAGAAGTGAAAGAGAAAGTGCCAACTTTACAACATGTAATTGTAGTGGGAGAAGAA
GAAGAATTTGTGAACATAAGTGATTTATATATAGATCCCGTTCCATTACCAGAAATTCAGCCAAGTGATGTTGCGTTTCT
CCAATTATCAGGGGGGACAACAGGACTTTCTAAATTAATTCCTCGAACGCATGATGATTATATTTATAGTTTACGTGTTA
GCGCTGAAATTTGTAATTTAAGTGCAAAGAGTGTCTATATGGCCGTTCTTCCAGTAGCACATAATTACCCGATGAGCTCT
CCTGGAACATTTGGAACTTTCTATGCGGGTGGAAAAGTAGTGCTGGCAACTGGAGGTAGTCCAGATGAGGCATTTGCACT
TATTGAAAAAGAAAAAGTTACGATTACTGCTCTCGTACCACCATTAGCAATGATATGGCTTGATGCTGCATCTTCACGTA
ATAACGATTTATCGAGTCTAGAAGTGATTCAAGTAGGTGGCGCGAAATTTAGTGCTGAAGTTGCAAAACGTATACGCCCT
ACATTTGGATGTACATTACAGCAAGTATTCGGTATGGCGGAAGGATTAGTGAACTATACAAGATTAGATGATCCGGAAGA
AATTATTATTTATACACAAGGTAGACCAATGTCTGCACTCGATGAAGTACGAGTTGTTGATGAAAATGACAACGATGTAA
AACCTGGCGAAGTAGGTAGTTTATTAACAAGAGGGCCATATACAATTCGTGGTTATTATAAAGCAGAAGAGCATAATGCA
CAATCGTTTACAAAGGATGGATTTTATCGCACAGGAGATCTTGTGAAAGTAAATGAACAAGGTTACATCATTGTAGAAGG
AAGAGATAAAGATCAAATTAATCGTGGTGGTGAGAAAGTTGCCGCGGAAGAAGTTGAAAATCATCTATTAGCACATGTTG
CAGTACATGATGTAGCGATTGTATCTATGCCTGACGATTATTTAGGAGAACGTACTTGTGCATTTGTTATAGCTCGCGGA
CAAGCTCCGGCAGTAAGTGAATTAAAAAAATTTTTAAGAGAGCGTGGTATAGCAGCTTATAAGATTCCAGATCGAATTGA
ATTTATTGAATCATTCCCGCAAACAGGCGTTGGAAAGGTAAGCAAAAAAGAACTACGTAAAGTCATTGCTGAAAAACTTA
TTACAGTAAAACAATAA

Protein sequence : 
MLTGYTKWPKEFANRYREEGCWLGETFGGMLRERAEKYGDQIAVVSGNTHITYSEFDKKVDRLASGLLNLGIKQEDRVVI
QLPNVIEFFEVCFALFRIGALPVFALPSHRSSEISYFCEFGEASAYVISDKALGFDYRKLAREVKEKVPTLQHVIVVGEE
EEFVNISDLYIDPVPLPEIQPSDVAFLQLSGGTTGLSKLIPRTHDDYIYSLRVSAEICNLSAKSVYMAVLPVAHNYPMSS
PGTFGTFYAGGKVVLATGGSPDEAFALIEKEKVTITALVPPLAMIWLDAASSRNNDLSSLEVIQVGGAKFSAEVAKRIRP
TFGCTLQQVFGMAEGLVNYTRLDDPEEIIIYTQGRPMSALDEVRVVDENDNDVKPGEVGSLLTRGPYTIRGYYKAEEHNA
QSFTKDGFYRTGDLVKVNEQGYIIVEGRDKDQINRGGEKVAAEEVENHLLAHVAVHDVAIVSMPDDYLGERTCAFVIARG
QAPAVSELKKFLRERGIAAYKIPDRIEFIESFPQTGVGKVSKKELRKVIAEKLITVKQ

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE CAA21394.1 - Virulence HPI Protein 4e-119 50
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 8e-120 50
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 3e-119 50
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 6e-120 50
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 5e-119 50
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 4e-119 50
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 4e-119 50
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 4e-119 50
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 4e-119 50

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
BCN_2291 YP_005104824.1 2,3-dihydroxybenzoate-AMP ligase VFG0168 Protein 1e-135 52
BCN_2291 YP_005104824.1 2,3-dihydroxybenzoate-AMP ligase VFG0932 Protein 1e-111 47
BCN_2291 YP_005104824.1 2,3-dihydroxybenzoate-AMP ligase VFG1817 Protein 2e-110 44