Gene Information

Name : Clocl_1964 (Clocl_1964)
Accession : YP_005046490.1
Strain : Clostridium clariflavum DSM 19732
Genome accession: NC_016627
Putative virulence/resistance : Virulence
Product : type III secretion system ATPase, FliI/YscN
Function : -
COG functional category : -
COG ID : -
EC number : 3.6.3.15
Position : 2255947 - 2257266 bp
Length : 1320 bp
Strand : -
Note : PFAM: ATP synthase alpha/beta family, beta-barrel domain; ATP synthase alpha/beta family, nucleotide-binding domain; TIGRFAM: ATPase FliI/YscN family; type III secretion apparatus H+-transporting two-sector ATPase; flagellar protein export ATPase FliI

DNA sequence :
ATGACAGGCATAAATTTTAAAAGGTATAAAGAAATTCTTGCAAAATCCGACTTTATTGAATACAAAGGGAAGGTATCGAA
AATTATAGGTCTTACAATCGAATCCAACGGCCCGGAAGTTAATATGGGTGAAATTTGTTTGATTCAATCGTTAAGAGATA
AATATGAAATTAAAGCCGAAGTTGTCGGATTCAGGGAAAATAAAGTTATTCTGATGCCGTTGGGTGATATGGCAGGAATC
GGGCCGGGAAGCAGTGTAGTAGCTACAGGAGAATATTTAAGTGCGGCTGTGGGTGAAAAATTAATCGGAAGAATTATTGA
CGGTCTGGGGAATCCCATAGATGAAAAAGGAGAAATAGAAACTGAAGGTTTTTATCCTGTAAACAACGATCCACCTCATC
CATTAATGAGAAATCGAATTTCCGAACCGCTCCCTTTGGGAATTAAAACAATAGACGGTCTGCTGACAATAGGAAAGGGG
CAGAGAGTAGGCATATTTGCAGGAAGCGGTGTGGGAAAAAGTACTTTGATAGGTATGATAGCCAGAAATACCAAAGCCGA
TATAAATGTAATCGCACTCATCGGAGAACGCGGCAGGGAAGTGCGTGAGTTTATAGAAAAAGACCTGAAAGAAGAAGGAT
TGAGCAGGTCGGTTGTCATTGTGGCAACTTCCGATCAACCGGCTCTTGTGAGACTAAAAGCTGCATTTATGGCTACAGCT
GTTGCTGAATATTTCAGAGATCAGGGAAAAGATGTGCTGCTGCTAATGGATTCTCTGACACGATTTGCCATGGCACAGAG
AGAAATAGGTTTGGCTGTTGGTGAGCCACCAGTGTCAAGGGGTTATACGCCTTCGGTGTTTTCGATAATGCCAAAGTTGC
TCGAGAGAGCAGGTAATTCAGAGAGGGGATCAATTACCGGGCTTTATACCGTTTTGGTAGATGGTGACGATTTGACCGAA
CCGGTAACCGATACGGCCAGAGGAATACTTGACGGTCATATTGTACTTTCCAGAGCTCTAGCCAATAGAAATCAATATCC
TGCTATAGATGTACTTGCAAGCGTAAGCAGGGTTATGTCCGATATAATAGATGACAGGCATAAAGAAATTGCCAACAAGA
TAAAAAAACTTATGGCAATATACAGGGATGCTGAGGACCTTATTAATATCGGTGCCTATGTCAAAGGCAGCAATGAAAAA
ATTGACCTGGCTATTGAGTATAACGATAAAATACTTGAGTTTATAGAACAGGGAACTCATGAAAAATACAGTTTTAATGA
AGTAATCGGAATGCTTGAGAGTTTTGCCGACATTTACTGA

Protein sequence :
MTGINFKRYKEILAKSDFIEYKGKVSKIIGLTIESNGPEVNMGEICLIQSLRDKYEIKAEVVGFRENKVILMPLGDMAGI
GPGSSVVATGEYLSAAVGEKLIGRIIDGLGNPIDEKGEIETEGFYPVNNDPPHPLMRNRISEPLPLGIKTIDGLLTIGKG
QRVGIFAGSGVGKSTLIGMIARNTKADINVIALIGERGREVREFIEKDLKEEGLSRSVVIVATSDQPALVRLKAAFMATA
VAEYFRDQGKDVLLLMDSLTRFAMAQREIGLAVGEPPVSRGYTPSVFSIMPKLLERAGNSERGSITGLYTVLVDGDDLTE
PVTDTARGILDGHIVLSRALANRNQYPAIDVLASVSRVMSDIIDDRHKEIANKIKKLMAIYRDAEDLINIGAYVKGSNEK
IDLAIEYNDKILEFIEQGTHEKYSFNEVIGMLESFADIY

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
lscN AAO18044.1 LscN Virulence TTSS locus Protein 1e-110 52
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 9e-104 50
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 2e-104 50
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-102 50
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-102 50
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-103 50
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-103 50
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 9e-104 50
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-103 50
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 9e-104 50
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 2e-86 48
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 2e-86 48
escN YP_003223475.1 translocator EscN Virulence LEE Protein 1e-89 48
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 3e-89 48
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 1e-89 48
escN AAK26715.1 EscN Virulence LEE Protein 1e-89 48
escN AAL57542.1 EscN Virulence LEE Protein 1e-89 48
escN CAI43874.1 EscN protein Virulence LEE Protein 1e-89 48
escN CAC81862.1 EscN protein Virulence LEE II Protein 8e-90 48
escN YP_003236088.1 translocator EscN Virulence LEE Protein 6e-89 48
escN NP_290268.1 hypothetical protein Virulence LEE Protein 4e-89 48
escN AAC38383.1 EscN Virulence LEE Protein 4e-89 48
ECs4568 NP_312595.1 EscN Virulence LEE Protein 4e-89 48
escN AAC31513.1 L0034 Virulence LEE Protein 3e-89 48
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 2e-86 48
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 9e-89 48
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 8e-86 47
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 8e-86 47
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 3e-86 47
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 4e-86 47
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-86 47
unnamed AAL06369.1 EscN Virulence LEE Protein 2e-87 47
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 1e-75 45
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 3e-75 44
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 5e-90 44
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 4e-83 44
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 5e-83 44
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 5e-83 44
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 1e-76 44
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 1e-76 43
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 1e-76 43
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 2e-72 42
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 2e-70 41
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 2e-70 41
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 2e-70 41
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 2e-70 41
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 3e-70 41
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 3e-71 41
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 3e-71 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Clocl_1964 YP_005046490.1 type III secretion system ATPase, FliI/YscN VFG0390 Protein 8e-113 54
Clocl_1964 YP_005046490.1 type III secretion system ATPase, FliI/YscN VFG2144 Protein 6e-111 53
Clocl_1964 YP_005046490.1 type III secretion system ATPase, FliI/YscN VFG0192 Protein 5e-115 53
Clocl_1964 YP_005046490.1 type III secretion system ATPase, FliI/YscN VFG0048 Protein 6e-109 53
Clocl_1964 YP_005046490.1 type III secretion system ATPase, FliI/YscN VFG1254 Protein 7e-101 51
Clocl_1964 YP_005046490.1 type III secretion system ATPase, FliI/YscN VFG0812 Protein 2e-89 48
Clocl_1964 YP_005046490.1 type III secretion system ATPase, FliI/YscN VFG0730 Protein 2e-89 48
Clocl_1964 YP_005046490.1 type III secretion system ATPase, FliI/YscN VFG2331 Protein 5e-88 47
Clocl_1964 YP_005046490.1 type III secretion system ATPase, FliI/YscN VFG0515 Protein 2e-86 47
Clocl_1964 YP_005046490.1 type III secretion system ATPase, FliI/YscN VFG2499 Protein 2e-91 47
Clocl_1964 YP_005046490.1 type III secretion system ATPase, FliI/YscN VFG1778 Protein 9e-74 43
Clocl_1964 YP_005046490.1 type III secretion system ATPase, FliI/YscN VFG0555 Protein 7e-71 41