Gene Information

Name : DSC_03140 (DSC_03140)
Accession : YP_004929322.1
Strain : Pseudoxanthomonas spadix BD-a59
Genome accession: NC_016147
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : -
Position : 467479 - 468885 bp
Length : 1407 bp
Strand : -
Note : COG1157 Flagellar biosynthesis/type III secretory pathway ATPase

DNA sequence :
ATGAACACCACTGCGCACCGCGCCGCCACGATCGAGGGCCTGCAGGGCGCACGCAACCTGCGCCTGGCCGAGCGGCTGCG
CGCGGCCGCGCCGGAGCCGCCGCACATGGGCCTGGTCCGCGAAGGCGTGCTGCGCCGCGCGGTCGGCCTGACCCTGGAAG
CCTCCGGCTGCAGCGCGCCGATGGGCTCGCGCTGCCGCGTGGCCACCTCCGGCGGACGCTGGGTCGATGCCGAGGTGGTC
GGCTTCTCCGGCGACCGCACCTACCTGATGCCGACCGGCGACCTGCAGGGCCTGCTGCCCAACGCGCGCGTGGTCTCGAT
CCATCGCAAGGGCGAAGTGGCCGTCGGCGAAGGCCTGCTCGGCCGGGTCATCGACAACGAAGGCACACCGCTGGACGGCA
AGGGCCCGATTCGCGCCGAACGCTACGTGGCGCTGGCCGGCACCCCGATCAACCCGCTCTCGCGCATGCCGATCAGCGAA
TCGCTCGATGTCGGCGTGCGCGCGATCAACGCCGTGCTGCCGATCGGCCGCGGCCAGCGCGTGGGCCTGTTCGCCGGCTC
GGGCGTGGGCAAATCCACGCTGCTGGGAATGATGACCCGCTTCACCGGGGCCGACGTCATCGTGGTCGGGCTGATCGGCG
AACGCGGCCGCGAAGTGCGCGATTTCGTCGAAGCCACGCTGGGCGAGGAAGGCCTGCGCCGCGCGGTCGTGGTCGCCGCG
CCGGCCGACCGTCCGCCGCTGGCGCGCCTGCATGGCGCGCTGCGCGCCACCGCGATCGCCGAGTGGTACCGCGACCAGGG
CCTGCAGGTGCTGCTGCTGATGGATTCGCTGACCCGCTTCGCCCAGGCCCAGCGCGAGATCGGGCTGTCGGTCGGCGAGC
CGCCGACCACCCGCGGCTATCCGCCCAGCGTGTTCGCCAAGCTCCCGGCCCTGGTGGAGCGTGCCGGCATGGGCGCCGAC
GGGCGCGGTTCGATCACCGCCTTCTATACCGTGCTGACCGAAGGCGATGACCAGCAGGAGCCGATCGCCGACGCCTCGCG
CGCCATCCTTGACGGCCACATCGTGCTGACCCGACGCATCGCCGATGCCGGCCAGTACCCGGCCATCGACATCGAGCAAT
CCGTCAGCCGGGTGATGCAGGACATCACCGACGCGCCCTGGCGCGAACACATCCGCAAGCTCAAGCGCCTGATCGCCGCC
TACAACGCCCAGCGCGACCTGATCGCGATCGGCGCCTACCAGCGCGGCAGCGATCCGAATGTGGACCAGGCGCTTGCGCG
CTGGCCGGCGATCCAGAATTTCCTCGGCCAGGACGTGCGCGAGTGCTCGGATGTGGCCGCCAGCCGCGATGCGCTCTCGC
GCCTGCTCGACGAGGGCAACGGCCAGCCGTTCCCGCTCACGCAGTAA

Protein sequence :
MNTTAHRAATIEGLQGARNLRLAERLRAAAPEPPHMGLVREGVLRRAVGLTLEASGCSAPMGSRCRVATSGGRWVDAEVV
GFSGDRTYLMPTGDLQGLLPNARVVSIHRKGEVAVGEGLLGRVIDNEGTPLDGKGPIRAERYVALAGTPINPLSRMPISE
SLDVGVRAINAVLPIGRGQRVGLFAGSGVGKSTLLGMMTRFTGADVIVVGLIGERGREVRDFVEATLGEEGLRRAVVVAA
PADRPPLARLHGALRATAIAEWYRDQGLQVLLLMDSLTRFAQAQREIGLSVGEPPTTRGYPPSVFAKLPALVERAGMGAD
GRGSITAFYTVLTEGDDQQEPIADASRAILDGHIVLTRRIADAGQYPAIDIEQSVSRVMQDITDAPWREHIRKLKRLIAA
YNAQRDLIAIGAYQRGSDPNVDQALARWPAIQNFLGQDVRECSDVAASRDALSRLLDEGNGQPFPLTQ

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 5e-83 51
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 3e-77 51
lscN AAO18044.1 LscN Virulence TTSS locus Protein 4e-73 50
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-82 49
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-82 49
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 7e-84 49
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 2e-82 49
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 1e-83 49
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-82 49
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 5e-84 49
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 5e-84 49
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 9e-71 48
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 9e-71 48
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 3e-71 47
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 4e-71 47
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-71 47
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 1e-63 46
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 7e-64 45
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 3e-64 45
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 3e-64 45
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 3e-61 45
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 3e-61 45
escN CAC81862.1 EscN protein Virulence LEE II Protein 9e-68 45
escN AAC38383.1 EscN Virulence LEE Protein 2e-67 45
escN YP_003236088.1 translocator EscN Virulence LEE Protein 3e-67 45
escN AAK26715.1 EscN Virulence LEE Protein 1e-67 45
escN AAL57542.1 EscN Virulence LEE Protein 1e-67 45
escN YP_003223475.1 translocator EscN Virulence LEE Protein 1e-67 45
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 1e-67 45
escN CAI43874.1 EscN protein Virulence LEE Protein 1e-67 45
unnamed AAL06369.1 EscN Virulence LEE Protein 2e-66 45
escN NP_290268.1 hypothetical protein Virulence LEE Protein 2e-67 45
ECs4568 NP_312595.1 EscN Virulence LEE Protein 2e-67 45
escN AAC31513.1 L0034 Virulence LEE Protein 1e-67 45
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 1e-67 45
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 1e-64 45
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 4e-64 44
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 7e-66 44
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 7e-63 44
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 4e-62 44
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 2e-62 44
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 3e-66 44
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 3e-66 44
invC AAS66861.1 InvC Not tested SSR-2 Protein 4e-55 42
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 3e-57 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
DSC_03140 YP_004929322.1 flagellum-specific ATP synthase VFG1254 Protein 2e-112 61
DSC_03140 YP_004929322.1 flagellum-specific ATP synthase VFG2331 Protein 6e-101 57
DSC_03140 YP_004929322.1 flagellum-specific ATP synthase VFG2499 Protein 7e-106 57
DSC_03140 YP_004929322.1 flagellum-specific ATP synthase VFG0192 Protein 8e-76 53
DSC_03140 YP_004929322.1 flagellum-specific ATP synthase VFG0390 Protein 2e-76 51
DSC_03140 YP_004929322.1 flagellum-specific ATP synthase VFG0048 Protein 2e-80 48
DSC_03140 YP_004929322.1 flagellum-specific ATP synthase VFG0515 Protein 2e-71 47
DSC_03140 YP_004929322.1 flagellum-specific ATP synthase VFG2144 Protein 4e-76 46
DSC_03140 YP_004929322.1 flagellum-specific ATP synthase VFG0730 Protein 9e-68 45
DSC_03140 YP_004929322.1 flagellum-specific ATP synthase VFG0812 Protein 6e-68 45
DSC_03140 YP_004929322.1 flagellum-specific ATP synthase VFG1778 Protein 4e-60 41