Gene Information

Name : fliI (MEALZ_0910)
Accession : YP_004916198.1
Strain : Methylomicrobium alcaliphilum 20Z
Genome accession: NC_016112
Putative virulence/resistance : Virulence
Product : flagellum-specific ATP synthase
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : -
Position : 1018082 - 1019446 bp
Length : 1365 bp
Strand : +
Note : Evidence 2a : Function of homologous gene experimentally demonstrated in an other organism; PubMedId : 1482109, 8491729; Product type e : enzyme

DNA sequence :
ATGAAGTCTTCGCTAGATCGAAACGAGATTTGGCTCGGTAAGTTAAAACCTTACGCCAAACGGTTGGTTCAGCCCCCGGA
ACTGATCGTGGAAGGCAAGTTATCCAGAATGGTCGGATTGACACTCGAGGCTGTCGGTTGTAGAGCGCCTATCGGTTCTC
GTTGTCAAGTACAAACTAAAGCGGGCAGGGTTATCGAAGCCGAAGTCGTCGGTTTTTCCGGCGATCGCTTATTTCTAATG
CCCACCGGTGATCCTTATGGCTTGGAGCCGGACTGTCGCGTCATTCCGATGAGTAAGGAGTATTTAGCTAGAGTCGGTTT
TAGCTTGCTGGGTCGAGTATTGGACGGGGCGGGTAATGCTTTGGACGGCAAGGGACCTTTGAAGACCGATGCGCAAGTGT
CGTTAACCGGTGTTCCTGTCAATCCGCTTAGCCGAAAGCCGATTCGCGAAGTGCTCGATGTCGGTGTCAGAGCGATCAAT
GCCTTGTTGTGTGTAGGGCGAGGGCAAAGGATGGGCTTATTTGCCGGAACCGGCGTCGGCAAGAGCGTTTTGCTGGGGAT
GATGACTAAGTTTACCAATGCCGACGTCGTCGTGGTGGGGCTTATCGGTGAACGGGGGCGTGAGGTTAACGAATTCGTAC
AAAAAATTCTGGGTACCGAAGGGCTTAGTCGCTCTGTCGTTGTTGCGACGCCGGCCGACTACCCGCCATTGATGCGGGCG
AATGGAGCATTGCTTGCGACCAGTATTGCTGAGTATTTCCGCGATCAGGGCTTGGATGTTTTACTGCTGATGGACTCGCT
GACTCGTTATGCTCAAGCCCATCGAGAAATCGCGCTCGCAATCGATGAACCGCCTGCCACTAAAGGTTACCCGCCTTCGG
TATTTGCTAAGTTGCCTCGGTTAGTTGAGCGTGCCGGGAACGGCGACGAAGGCGGCGGCTCGATTACGGCTTTTTATACC
GTACTGACCGAAGGCGACGATCCCAACGATCCTGTAGCCGACTCGGCTCGCGGCGTATTGGATGGGCATATCGTGCTAAC
TCGAGAGCTTGCGGAGTCCGGACATTTTCCCGCGATCGATATCGAGGCGTCGGTCAGTAGGGTTTTGCCGGATATTGTCG
ATGACGATCATTTACGAATGGTGCGCAAATTGAGAGGCCTGTATTCTCTGTATCAGCAAAATAGAGACCTCATCAGCGTC
GGCGCCTACAAACCGGGTTCGAATCCGAATATCGATTTGGCGATTGAGAAAATAGCAGCGATTAGGAATTTTTTGCAACA
AGATATGAATGAGCCTGTGGATTTGAGTCGAAGTATCTTGGAATTGTCCGACTTGCTTGGGAATGAGGTGAAAGGTGAAA
GGTGA

Protein sequence :
MKSSLDRNEIWLGKLKPYAKRLVQPPELIVEGKLSRMVGLTLEAVGCRAPIGSRCQVQTKAGRVIEAEVVGFSGDRLFLM
PTGDPYGLEPDCRVIPMSKEYLARVGFSLLGRVLDGAGNALDGKGPLKTDAQVSLTGVPVNPLSRKPIREVLDVGVRAIN
ALLCVGRGQRMGLFAGTGVGKSVLLGMMTKFTNADVVVVGLIGERGREVNEFVQKILGTEGLSRSVVVATPADYPPLMRA
NGALLATSIAEYFRDQGLDVLLLMDSLTRYAQAHREIALAIDEPPATKGYPPSVFAKLPRLVERAGNGDEGGGSITAFYT
VLTEGDDPNDPVADSARGVLDGHIVLTRELAESGHFPAIDIEASVSRVLPDIVDDDHLRMVRKLRGLYSLYQQNRDLISV
GAYKPGSNPNIDLAIEKIAAIRNFLQQDMNEPVDLSRSILELSDLLGNEVKGER

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 1e-91 50
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 7e-91 49
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 5e-91 49
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 5e-92 49
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-92 48
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 2e-92 48
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 2e-92 48
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 1e-83 48
lscN AAO18044.1 LscN Virulence TTSS locus Protein 6e-83 47
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 8e-90 47
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 8e-90 47
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 6e-71 47
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 6e-71 47
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 9e-73 47
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 3e-72 47
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 3e-72 47
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-72 47
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 1e-72 47
escN AAC38383.1 EscN Virulence LEE Protein 2e-73 46
escN CAC81862.1 EscN protein Virulence LEE II Protein 2e-73 46
escN NP_290268.1 hypothetical protein Virulence LEE Protein 3e-73 46
unnamed AAL06369.1 EscN Virulence LEE Protein 2e-73 46
escN AAK26715.1 EscN Virulence LEE Protein 3e-73 46
ECs4568 NP_312595.1 EscN Virulence LEE Protein 3e-73 46
escN AAL57542.1 EscN Virulence LEE Protein 3e-73 46
escN YP_003223475.1 translocator EscN Virulence LEE Protein 3e-73 46
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 2e-73 46
escN AAC31513.1 L0034 Virulence LEE Protein 2e-73 46
escN YP_003236088.1 translocator EscN Virulence LEE Protein 9e-74 46
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 2e-73 46
escN CAI43874.1 EscN protein Virulence LEE Protein 2e-73 46
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 5e-69 45
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 5e-69 45
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 7e-67 44
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 7e-65 43
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 1e-68 43
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 2e-69 43
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 2e-67 43
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 2e-67 43
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 2e-67 43
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 2e-67 43
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 1e-65 42
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 1e-65 42
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 2e-73 41
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 3e-70 41
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 3e-70 41
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 2e-70 41
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 4e-65 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
fliI YP_004916198.1 flagellum-specific ATP synthase VFG1254 Protein 1e-132 63
fliI YP_004916198.1 flagellum-specific ATP synthase VFG2499 Protein 4e-121 59
fliI YP_004916198.1 flagellum-specific ATP synthase VFG2331 Protein 1e-109 57
fliI YP_004916198.1 flagellum-specific ATP synthase VFG0192 Protein 1e-85 48
fliI YP_004916198.1 flagellum-specific ATP synthase VFG0048 Protein 1e-88 47
fliI YP_004916198.1 flagellum-specific ATP synthase VFG0515 Protein 5e-73 47
fliI YP_004916198.1 flagellum-specific ATP synthase VFG2144 Protein 7e-87 46
fliI YP_004916198.1 flagellum-specific ATP synthase VFG0390 Protein 5e-82 46
fliI YP_004916198.1 flagellum-specific ATP synthase VFG0730 Protein 1e-73 46
fliI YP_004916198.1 flagellum-specific ATP synthase VFG0812 Protein 1e-73 46
fliI YP_004916198.1 flagellum-specific ATP synthase VFG1008 Protein 3e-68 44
fliI YP_004916198.1 flagellum-specific ATP synthase VFG0555 Protein 1e-67 43
fliI YP_004916198.1 flagellum-specific ATP synthase VFG2459 Protein 2e-68 41
fliI YP_004916198.1 flagellum-specific ATP synthase VFG1778 Protein 2e-66 41