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Name : pyk (TEH_08980) Accession : YP_004886389.1 Strain : Tetragenococcus halophilus NBRC 12172 Genome accession: NC_016052 Putative virulence/resistance : Virulence Product : pyruvate kinase Function : - COG functional category : G : Carbohydrate transport and metabolism COG ID : COG0469 EC number : 2.7.1.40 Position : 899630 - 901396 bp Length : 1767 bp Strand : + Note : The N-terminus of this ORF was identified by Edman sequencing. DNA sequence : ATGAAAAAAACAAAAATCGTAAGTACCTTAGGACCTGCTAGTAATTCGGTAGATACGATCGCAAAATTGATTGAAGCTGG AGCGAACGTTTTTCGTTTTAACTTTTCTCATGGTGATCACGATGAGCATTTAGATCGCATGGATAAAGTGCATCAAGCAG TTGAAAGAACTGGAATAGACGTAGGGATTCTTTTGGATACAAAAGGTGCTGAAATCCGGACAACAGCTCAAGCTACTGAA ACAGGTAAGATCCCTTTTGAGATCGGCGATAGAGTACGTATTGCAATGGATGCGGAATTAGAAGGTACAAAAGACAAAAT TTCTGTTACTTATGAAGGTCTTTTTGATGATGTTCATGAAGGTGGACACGTGTTATTTGATGATGGTCTAATTGACATGC TTATTTTAGAAAAAGATGAAAGCAATCGGGAACTAGTTACTGAAGTGCAAAACCCTGGGATTTTGGGTTCACGTAAAGGC GTGAATGCGCCAGGTGTTTCTATCAGCTTACCTGGAATCACTGATAAAGATGCTGATGATATTCGTTTTGGCTTGCAGCA TAATATTAACTATATTGCAGCAAGTTTTGTACGTAAAGCACAAGACGTATTAGATATTCGGGAGATTTTAGAAGAAACAG GAATGACTCATGTTCAAATCTTTCCTAAGATTGAATCTCAAGAAGGTATTGATAATATTGATGAAATACTGAAAGTTTCC GACGGTATTATGGTTGCCCGAGGCGACATGGGTGTTGAAATTCCTTCTGAATTAGTCCCTATGGCGCAAAAAAATATTAT TAAAAAATGTAATGCTGCAGGTAAATCAGTTATTACAGCAACACAAATGTTAGAATCTATGCAAGAAAATCCACGTCCGA CCCGTGCAGAAGCTTCTGACGTTGCTAACGCTGTTTTTGACGGAACAGACGCAACTATGCTTTCTGGCGAATCAGCTAAT GGAAGCTATCCAGTTGAAGCTGTTACCACTATGGCTCGTATTGATGTAGAAGCAGAAAAATCAATGTTACAACTTGGTTC TTACCAAATTAACCAATTTGATAAAACGGATGTTACGGAAACAATTGGTCTTTCTGTAGCACGTGCTGCCAAGAATTTGG GTGTTAAATGTATCGTAGCTGCGACTGAATCTGGTCATACAGCTAAGATGATTTCAAAATATCGTCCAGATGCAGATATT TTGGCTGTAACTTTTGATGACCATACTAAACGCGGTCTGATGTTAAACTGGGGCGTTTATCCAACAGTTACAGAAAAGCC TGAAACAACGGATGATATGTTTGAATTAGCAACAAAGAAAACATTGGAATTAGGTTTTGCTAAAGAAGGCGATTCGATTT TGATTACTGCTGGTGTACCTGTGGGCGAACGTGGTACAACAAACGTTATGAAGATCCAAATGATTGGCTCAAAATTAATT GAAGCCCAAGGCGTTGGCGATCATTCAGTAGTTGCTAATACTGTTGTAGCTAAGAATGCTGAAGAAGCTGTGGCTAAAGC TAAAGATGGAATGGTTCTAGTTGTACCATCAACAGATAAAGAATACATGCCAGCTATTGAAAAAGCAGCTGCTTTAGTTG TAGAAGAAAGTGGTTTGACTTCACATGCGGCAGTTGTTGGTGTAACCCAAGACTTACCTGTCATTGTGGGTGCTAAAGAT GCTTTGAATATTATTAATGATGGTGAATTAGTAACAATTGATTCTCGTCGTGGCATTGTTTACCGTGGAGAAACGATGGC AGTTTAA Protein sequence : MKKTKIVSTLGPASNSVDTIAKLIEAGANVFRFNFSHGDHDEHLDRMDKVHQAVERTGIDVGILLDTKGAEIRTTAQATE TGKIPFEIGDRVRIAMDAELEGTKDKISVTYEGLFDDVHEGGHVLFDDGLIDMLILEKDESNRELVTEVQNPGILGSRKG VNAPGVSISLPGITDKDADDIRFGLQHNINYIAASFVRKAQDVLDIREILEETGMTHVQIFPKIESQEGIDNIDEILKVS DGIMVARGDMGVEIPSELVPMAQKNIIKKCNAAGKSVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESAN GSYPVEAVTTMARIDVEAEKSMLQLGSYQINQFDKTDVTETIGLSVARAAKNLGVKCIVAATESGHTAKMISKYRPDADI LAVTFDDHTKRGLMLNWGVYPTVTEKPETTDDMFELATKKTLELGFAKEGDSILITAGVPVGERGTTNVMKIQMIGSKLI EAQGVGDHSVVANTVVAKNAEEAVAKAKDGMVLVVPSTDKEYMPAIEKAAALVVEESGLTSHAAVVGVTQDLPVIVGAKD ALNIINDGELVTIDSRRGIVYRGETMAV |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| pykF | CAA68205.1 | pyruvate kinase like protein | Not tested | SPI-2 | Protein | 3e-89 | 47 |
| STY1744 | NP_456147.1 | pyruvate kinase | Not tested | SPI-2 | Protein | 3e-89 | 47 |
| pykF | YP_216386.1 | pyruvate kinase | Not tested | SPI-2 | Protein | 2e-89 | 47 |
| pykF | NP_805051.1 | pyruvate kinase | Not tested | SPI-2 | Protein | 2e-89 | 47 |
| Gene | GenBank Accn | Product | ID of source DB | Alignment Type | E-val | Identity |
| pyk | YP_004886389.1 | pyruvate kinase | VFG0479 | Protein | 9e-90 | 47 |
