Gene Information

Name : GYO_3489 (GYO_3489)
Accession : YP_004878698.1
Strain : Bacillus subtilis TU-B-10
Genome accession: NC_016047
Putative virulence/resistance : Virulence
Product : 2,3-dihydroxybenzoate-AMP ligase
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : 2.7.7.58
Position : 3230654 - 3232273 bp
Length : 1620 bp
Strand : -
Note : -

DNA sequence : 
ATGCTGAATGGATTTACTCCTTGGCCTGAAGAGTTAGCTGAGACATATCGGAAGAACGGCTGCTGGGCGGGCGAGACGTT
TGGAGATTTGTTAAGGGACCGGGCTGCTAAACATGGCGATCGCATTGCAATTACATGCGGAAACACCCATTGGAGCTATC
AGGAGCTTGACACAAGGGTTGATCAACTGGCTGCCGGTTTTCAGAAGCTCGGCATTCAACAGATGGATCGTGTCGTGGTG
CAGCTGCCGAATATCGCAGAGTTTTTTGAAGTCATTTTCGCACTCTTCCGTTTGGGGGCGCTTCCGGTTTTCGCACTGCC
TTCTCATCGAAGCAGTGAAATTACATATTTTTGCGAGTTTGCTGAAGCGGCTGCTTATATTATTCCCGACTCCTATTCCG
GTTTTGACTACCGTTCACTTGCCAGACAGGTTCAAAGCAAACTGCCATCTTTAAAACATGTCATTGTTGCAGGTGAGGCA
GAGGAATTTTTGCCACTGGAGGCTTTGCATACAGAACCTGTAAAACTACCTGAAGTCAAATCTTCTGATGTGGCGTTCCT
TCAGCTTTCTGGAGGAAGCACCGGCTTATCAAAACTGATCCCGCGGACTCACGATGACTACATATACAGTCTGCGGCGGA
GTGCAGAGGTTTGCTGGCTCGACCACAGCACGGTGTACTTGGCAGCATTGCCGATGGCGCATAATTATCCGCTGAGCTCG
CCTGGAGTTCTCGGCGTGTTATACGCCGGAGGAAGAGTGGTATTATCTCCTTCTCCAAGTCCTGATGACGCATTTCCTTT
GATTGAACGTGAACAAGTCACAATTACCGCTCTTGTTCCGCCCCTTGCAATGGTATGGATGGATGCGGCATCCTCACGCG
ATGACAATCTATCCAGCCTTCAAGTTCTGCAGGTCGGCGGCGCCAAGTTCAGTGCTGAAGCTGCGCGCAAGGTTAAAGCT
GTTTTCGGATGCACGCTGCAGCAGGTGTTCGGAATGGCAGAGGGTCTCGTCAATTATACGAGACTGGATGACCCTGAGGA
AGTCATTGTCAACACCCAAGGAAAACCGATGTCCCCATATGATGAAGTACGTGTTTGGGATGATCAGGATCGTGACGTAA
AACCTGGTGAAACAGGGCATCTGCTGACACGGGGGCCGTACACGATTCGGGGTTACTATAAAGCAGAAGAGCACAATGCT
GCTTCTTTTACTGAGGACGGTTTTTACCGCACGGGTGATATCGTCAGGCTGACATCAGACGGCTATATTGTCGTTGAAGG
CAGGGCGAAGGATCAAATTAACCGCGGGGGAGAAAAAGTGGCGGCTGAAGAGGTAGAAAATCATCTTCTGGCGCATCCGG
CTGTCCATGATGCCGCAATGGTCTCAATGCCTGACCAATTTCTTGGCGAAAGATCTTGTGTGTTCATTATTCCCCGGGAT
GAAGCCCCGAAAGCCGCAGAGCTCAAAGCTTTTTTGAGAGAGCGCGGACTGGCGGCATACAAAATCCCCGATCGCATTGA
ATTTGTCGAATCCTTTCCGCAGACAGGGGTAGGAAAAGTCAGCAAAAAAGCGCTTCGTGAAGCCATTGCCGAGAAGCTTC
TTGCAGGATCAAAAAAATAA

Protein sequence : 
MLNGFTPWPEELAETYRKNGCWAGETFGDLLRDRAAKHGDRIAITCGNTHWSYQELDTRVDQLAAGFQKLGIQQMDRVVV
QLPNIAEFFEVIFALFRLGALPVFALPSHRSSEITYFCEFAEAAAYIIPDSYSGFDYRSLARQVQSKLPSLKHVIVAGEA
EEFLPLEALHTEPVKLPEVKSSDVAFLQLSGGSTGLSKLIPRTHDDYIYSLRRSAEVCWLDHSTVYLAALPMAHNYPLSS
PGVLGVLYAGGRVVLSPSPSPDDAFPLIEREQVTITALVPPLAMVWMDAASSRDDNLSSLQVLQVGGAKFSAEAARKVKA
VFGCTLQQVFGMAEGLVNYTRLDDPEEVIVNTQGKPMSPYDEVRVWDDQDRDVKPGETGHLLTRGPYTIRGYYKAEEHNA
ASFTEDGFYRTGDIVRLTSDGYIVVEGRAKDQINRGGEKVAAEEVENHLLAHPAVHDAAMVSMPDQFLGERSCVFIIPRD
EAPKAAELKAFLRERGLAAYKIPDRIEFVESFPQTGVGKVSKKALREAIAEKLLAGSKK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 5e-121 50
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 5e-121 50
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 5e-121 50
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 5e-121 50
ybtE CAA21394.1 - Virulence HPI Protein 5e-121 50
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 3e-121 50
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 4e-121 50
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 2e-121 50
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 7e-121 50

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
GYO_3489 YP_004878698.1 2,3-dihydroxybenzoate-AMP ligase VFG0168 Protein 2e-140 55
GYO_3489 YP_004878698.1 2,3-dihydroxybenzoate-AMP ligase VFG0932 Protein 4e-114 49
GYO_3489 YP_004878698.1 2,3-dihydroxybenzoate-AMP ligase VFG1817 Protein 7e-114 45