Gene Information

Name : Bcoa_2517 (Bcoa_2517)
Accession : YP_004860475.1
Strain : Bacillus coagulans 36D1
Genome accession: NC_016023
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : -
Position : 2747045 - 2748805 bp
Length : 1761 bp
Strand : +
Note : KEGG: afl:Aflv_0500 pyruvate kinase; TIGRFAM: Pyruvate kinase; PFAM: Pyruvate kinase, barrel; Pyruvate kinase, alpha/beta; PEP-utilising enzyme, mobile region

DNA sequence :
ATGAAAAAAACCAAAATTGTATGTACAATCGGGCCTGCCAGTGAAAGTGTTGAAATGCTTGAAAGATTAATGGCAAACGG
GATGGATGTTTGCCGCCTGAACTTCTCGCACGGCAGCCACGAGGAACATCTTGCCCGGATTAAAAATATCCGCGAAGCTG
CAAAAAACCAAAACAAAACGATCGGGCTTCTGCTTGATACAAAGGGCCCGGAAATCCGCACCCACGATATGAAAGATGGC
GGATTTGAGCTCGTTGAAGGCATGACGCCGGTCATTTCAATGACAGAAGTGCTCGGGACAACGGAAAAATTTTCGGTCAC
GTATGAAGGGCTGATTGATGATGTGCACGTTGGCTCTAAAATTCTGCTTGATGATGGTTTGATTGAACTGGAAGTGACGG
CCATCGATAAAAACGCCGGTGAAATCCATACAAAAGTGCTGAACCGCGGCGTTTTGAAAAACAAAAAGGGTGTCAACGTC
CCGGGTGTCTCCGTGAACCTTCCGGGCATTACCGAAAAAGACGTGAGCGATATCCTGTTCGGGCTTGAACAAGGCATTGA
CTTCATTGCGGCTTCATTTGTACGCCGGCCGTCTGACGTTTTGGAAATTCGCCAGCTCCTTGAAGAACACGATGCTTTAC
ATGTGAAAATTTTCCCTAAAATTGAAAACCAGGAAGGCGTCGACAACATCGATGAAATCCTAGCTGTATCGGACGGCTTA
ATGGTTGCCCGCGGTGACCTCGGCGTTGAAATTCCGACCGAAGCGGTACCGCTCGTGCAAAAAGAGCTGATCAGAAAATG
CAATACGCTCGGCAAACCGGTCATTACCGCAACGCAAATGCTGGATTCGATGCAGCGCAACCCGCGCCCGACCCGCGCAG
AAGCAAGCGACGTGGCCAACGCCATTTTCGACGGCACGGATGCAATCATGCTTTCCGGCGAAACAGCAGCCGGAAAATAT
CCTGCTGAAGCGGTTAAGACGATGTACAATATCGCGGTCCATGTGGAAAAAGCGATTAACCACCGCGATATTCTGAACAA
ACGCAGCAAGAGCACGGACCATAATATGACAGACGCCATTTGCCAGTCCGTTGCCCATACGGCTTTAAATCTTGATGTGA
ATGCCATTATTGCGCCAACCGAAAGCGGCTATACGGCGCGCATGATCTCCAAATACCGCCCGGCAGCCCCGATTATTGCT
GTCACGAGCGATCCGAAAGTGCAGCGCGGCTTAACCGTTGTGTCCGGCGTATACCCGCAATTGGGCACAAAGGCAAACAA
TACGGATGAAATGCTTGAAATCGCAGTGGATGAAGCATTGAAATCCGATATCGTCCATCACGGCGACCTTGTGATTATCA
CAGCCGGCGTCCCTGTTGGTGGGAAAGGCACAACCAACCTGATGAAAGTGCACCTGATCGGCGATATATTGGCAAAAGGC
CAGGGAATCGGCAGAAAATCGGCATTTGGTCCGGTCGTTGTTGCTGAAAGCCCAGAAGAAGCAAACGCAAAAGCAACAGA
AGGCTGTGTGCTCATCACGAGAACGACCGACAAAGAAATCATGCCGGCCATTGAAAAATGTGCCGCGCTGATTACGGAAG
AAGGCGGCTTGACAAGCCATGCTGCAGTTGTTGGCATCAATGTCGGCATTCCGGTCATTGTCGGTGTTGAAAAAGCCGTT
TCCATTTTTAAAGACGGGCAGGAAGTTACGGTAGATGCAGCAACCGGCTCGGTTTACAACGGCCATGCGACTGTATTGTA
A

Protein sequence :
MKKTKIVCTIGPASESVEMLERLMANGMDVCRLNFSHGSHEEHLARIKNIREAAKNQNKTIGLLLDTKGPEIRTHDMKDG
GFELVEGMTPVISMTEVLGTTEKFSVTYEGLIDDVHVGSKILLDDGLIELEVTAIDKNAGEIHTKVLNRGVLKNKKGVNV
PGVSVNLPGITEKDVSDILFGLEQGIDFIAASFVRRPSDVLEIRQLLEEHDALHVKIFPKIENQEGVDNIDEILAVSDGL
MVARGDLGVEIPTEAVPLVQKELIRKCNTLGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAIMLSGETAAGKY
PAEAVKTMYNIAVHVEKAINHRDILNKRSKSTDHNMTDAICQSVAHTALNLDVNAIIAPTESGYTARMISKYRPAAPIIA
VTSDPKVQRGLTVVSGVYPQLGTKANNTDEMLEIAVDEALKSDIVHHGDLVIITAGVPVGGKGTTNLMKVHLIGDILAKG
QGIGRKSAFGPVVVAESPEEANAKATEGCVLITRTTDKEIMPAIEKCAALITEEGGLTSHAAVVGINVGIPVIVGVEKAV
SIFKDGQEVTVDAATGSVYNGHATVL

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 1e-100 51
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 5e-101 51
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 5e-101 51
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 1e-100 51

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Bcoa_2517 YP_004860475.1 pyruvate kinase VFG0479 Protein 2e-101 51