Gene Information

Name : Entas_1092 (Entas_1092)
Accession : YP_004827623.1
Strain : Enterobacter asburiae LF7a
Genome accession: NC_015968
Putative virulence/resistance : Virulence
Product : 2,3-dihydroxybenzoate-AMP ligase
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : -
Position : 1190510 - 1192120 bp
Length : 1611 bp
Strand : +
Note : KEGG: enc:ECL_03107 enterobactin 2,3-dihydroxybenzoate-AMP ligase/S-dihydroxybenzoyltransferase; TIGRFAM: 2,3-dihydroxybenzoate-AMP ligase; PFAM: AMP-dependent synthetase/ligase

DNA sequence : 
ATGACTGTCCCCTTTACCCGTTGGCCGGACGAGTTTGCCCGCCGCTATCGTGAAAAAGGCTACTGGCAGGATCTGCCTCT
GACCCGCATTCTGACCCGCCACGCTAATAATGACGCGGTGGCCATCATCGACGGTGAGCGCCGTTATACCTACCGCCAGT
TTAATGAGGCGGTCGATAACCTTGCCAGTTCGTTACAATCCCGGGGGCTGAAACGTGGCGATACCGCGCTGGTGCAACTG
GGAAATGTGGCTGAGTTTTACATCACCTTCTTTGCGCTGTTACAGGCGGGTGTTGCGCCCGTTAACGCGCTCTTCAGCCA
TCAGCGCAGCGAGCTGAATGCCTATGCGATGCAGATCAAACCAGCATTGCTGATTGCCGATCGTGATCACACGCTGTTTG
CCGGCGATGAGTTCCTCACCGCATTTGTGAATGAGCACCGCTCGGTGCGCGTGGTATTGCTGCGTGGCGATAACGGCGAG
CACGCACTGGACGCGGCGATTGCCCGTCCGACGGATAATTTCATCCCCAACCCGACACCTGCCGACGAAGTGGCGTTCTT
CCAGCTTTCTGGCGGAAGTACCGGCACGCCGAAGCTGATCCCGCGTACCCACAACGATTACGACTACAGCATTCGTCGCA
GTAACGAGATTTGCGGTATTACCGCAGACACTCGTTATCTGAACGCGCTGCCCGCTGCGCACAACTACGCCATGAGCTCG
CCGGGATCGCTTGGCATCTTTACGGCAGGTGGCTGCGTGGTGCTGGCGAACGATCCGAGCGCCACCCTCTGTTTCCCGCT
GATTGAAAAACACCAGATTAACGTGACCTCGCTGGTGCCGCCTGCGGTCAGCCTGTGGCTCCAGGCGATTGCCGAAGGCG
CGGGCAACGCGCAGCTGCGTTCGCTGAAGCTGTTACAGGTGGGGGGCGCACGCCTCTCTGCAACGCTTGCCGCACGCATT
CCGGCAGAGATTGGCTGCCAGCTCCAGCAGGTATTTGGTATGGCGGAAGGGCTGGTGAACTACACCGCGCTTGACGATTC
CCCGGAACGCATCATGAACACCCAGGGCCGCCCGATGTGCCCTGATGACGAAGTGTGGGTAGCCGATGAGCATGGCAACC
CGTTACCGCGCGGTGAAGTGGGGCGGCTGATGACGCGCGGACCATATACCTTCCGTGGGTATTTCAACAGCCCGGCGCAC
AACGCCAGCGCCTTTGATGCCAACGGCTTTTACTGTTCCGGCGATCTGATCGCCATTGACGAGCAGGGCTACATCACCGT
GCAGGGGCGCGAGAAAGATCAGATCAACCGTGGTGGAGAGAAGATCGCCGCAGAAGAGATCGAAAACCTGCTGCTGCGCC
ACGAGTCGGTGATCCACGCGGCGCTGGTAAGCATGGAAGACAGCCTGCTGGGTGAGAAAAGCTGCGCGTATCTGGTGGTG
AAACAGCCGCTGCGCGCGGTTGAAGTGCGTCGCTTCCTGCGCGAACAGGGCGTTGCCGAATTCAAACTGCCGGACCGCGT
GGAGAGCGTTGATGCGCTCCCGTTAACGCCCGTCGGCAAAGTCGATAAGAAACAGTTGCGCCTGTGGCTCGCTGAACGCG
CCCAGGGCTGA

Protein sequence : 
MTVPFTRWPDEFARRYREKGYWQDLPLTRILTRHANNDAVAIIDGERRYTYRQFNEAVDNLASSLQSRGLKRGDTALVQL
GNVAEFYITFFALLQAGVAPVNALFSHQRSELNAYAMQIKPALLIADRDHTLFAGDEFLTAFVNEHRSVRVVLLRGDNGE
HALDAAIARPTDNFIPNPTPADEVAFFQLSGGSTGTPKLIPRTHNDYDYSIRRSNEICGITADTRYLNALPAAHNYAMSS
PGSLGIFTAGGCVVLANDPSATLCFPLIEKHQINVTSLVPPAVSLWLQAIAEGAGNAQLRSLKLLQVGGARLSATLAARI
PAEIGCQLQQVFGMAEGLVNYTALDDSPERIMNTQGRPMCPDDEVWVADEHGNPLPRGEVGRLMTRGPYTFRGYFNSPAH
NASAFDANGFYCSGDLIAIDEQGYITVQGREKDQINRGGEKIAAEEIENLLLRHESVIHAALVSMEDSLLGEKSCAYLVV
KQPLRAVEVRRFLREQGVAEFKLPDRVESVDALPLTPVGKVDKKQLRLWLAERAQG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 2e-89 42
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-89 42
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-89 42
ybtE CAA21394.1 - Virulence HPI Protein 2e-89 42
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 4e-89 42
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 2e-89 42
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 5e-89 42
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 3e-89 42
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-89 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Entas_1092 YP_004827623.1 2,3-dihydroxybenzoate-AMP ligase VFG0932 Protein 0.0 80
Entas_1092 YP_004827623.1 2,3-dihydroxybenzoate-AMP ligase VFG0168 Protein 2e-110 45