Gene Information

Name : Thewi_2554 (Thewi_2554)
Accession : YP_004821163.1
Strain : Thermoanaerobacter wiegelii Rt8.B1
Genome accession: NC_015958
Putative virulence/resistance : Resistance
Product : D-alanine--D-alanine ligase
Function : -
COG functional category : M : Cell wall/membrane/envelope biogenesis
COG ID : COG1181
EC number : -
Position : 2590889 - 2591968 bp
Length : 1080 bp
Strand : -
Note : TIGRFAM: D-alanine--D-alanine ligase; HAMAP: D-alanine--D-alanine ligase; KEGG: tbo:Thebr_0193 D-alanine/D-alanine ligase; PFAM: D-alanine--D-alanine ligase, C-terminal; D-alanine--D-alanine ligase, N-terminal

DNA sequence :
ATGGCGAAGAAGAAAATAGCTGTTTTATTTGGTGGACAATCGGGAGAACATGAAGTCTCTTTAATGTCTGCAAAGTCTAT
AATAAACAACCTTGATAAAGATAAATACGAGATTTACATGATAGGTATTACAAAAAAAGGAGAATGGTATTTATATAGAG
GAGATGTAGAAAAAATAGAAACCGGAGAATGGGAAAAAGAAGGAATTCCTGCAACTATGGGAGCTTCTACGAAGTATAGA
GGAATAATAACTTTCGAAGACGGAAAAAATGGTTTTTATCCTATAGACGTGGTATTTCCTGTTTTGCATGGACCTAATGG
AGAAGATGGGACTATACAAGGGCTTTTAGAATTATTAGATATACCTTATGTAGGAGCTAATGTGCTTTCTTCTGCTTTAT
GCATGGATAAAGTTTTCACAAAAAGGATTTTTAAGGAAGCGGGGCTACCTACACCTGATTTTGTAGTAGTATATAGAAAA
GAGATTGAAGACTTAGAAGCTATAAAGAAAAAAGTTGAACACTTGGGATATCCGTGCTTTGTAAAACCTGCTAATTTAGG
TTCTAGTGTTGGTATTACCAAAGTCCACAATGAGGAAGAACTTCCTGGGGCCTTGAAATTAGCTGCAAAATATGATAGAA
AATTGTTGATAGAAAGAGGAATAGATGCTAGGGAAATTGAATGTTCCGTGCTAGGTAACGAAAATCCGCAAGCCTCTATT
GCAGGAGAAATTGTTCCTTCTAATGAATTTTATGATTATAATGCTAAATATTTTGATGGGGGTAAATCTCTTCTCTTAAT
ACCTGCACCACTTCCAGATGAAAAAATGGAAGAGGTAAGACAATTGGCTATAAAAGCATATAAGGCGTTGGATTTGAGAG
GTATGGCGCGAGTAGACTTTTTGATGGATAGAAACACAGGTACCCTTTATCTAAATGAAGTTAATACCATTCCTGGGTTT
ACGAAAATAAGCATGTATCCTAAACTGTGGGAAGTAAGTGGAAAGCCGTATTCAATATTATTGGACGAACTTATAAACTT
AGCGATAGAGAGCTATAATGAAAAATGTAGGGAATGGTGA

Protein sequence :
MAKKKIAVLFGGQSGEHEVSLMSAKSIINNLDKDKYEIYMIGITKKGEWYLYRGDVEKIETGEWEKEGIPATMGASTKYR
GIITFEDGKNGFYPIDVVFPVLHGPNGEDGTIQGLLELLDIPYVGANVLSSALCMDKVFTKRIFKEAGLPTPDFVVVYRK
EIEDLEAIKKKVEHLGYPCFVKPANLGSSVGITKVHNEEELPGALKLAAKYDRKLLIERGIDAREIECSVLGNENPQASI
AGEIVPSNEFYDYNAKYFDGGKSLLLIPAPLPDEKMEEVRQLAIKAYKALDLRGMARVDFLMDRNTGTLYLNEVNTIPGF
TKISMYPKLWEVSGKPYSILLDELINLAIESYNEKCREW

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
vanB NP_815951.1 D-alanine--D-lactate ligase Not tested Not named Protein 8e-56 41

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Thewi_2554 YP_004821163.1 D-alanine--D-alanine ligase AF277571.gene.p01 Protein 9e-56 43
Thewi_2554 YP_004821163.1 D-alanine--D-alanine ligase AF130997.1.orf4.gene Protein 8e-54 42
Thewi_2554 YP_004821163.1 D-alanine--D-alanine ligase AY489045.gene.p01 Protein 4e-55 42
Thewi_2554 YP_004821163.1 D-alanine--D-alanine ligase U35369.1.gene6.p01 Protein 3e-56 41
Thewi_2554 YP_004821163.1 D-alanine--D-alanine ligase AE016830.1.gene2250. Protein 3e-56 41
Thewi_2554 YP_004821163.1 D-alanine--D-alanine ligase U00456.gene.p01 Protein 3e-56 41
Thewi_2554 YP_004821163.1 D-alanine--D-alanine ligase AF162694.gene.p01 Protein 2e-55 41