Gene Information

Name : Calla_1828 (Calla_1828)
Accession : YP_004799387.1
Strain : Caldicellulosiruptor lactoaceticus 6A
Genome accession: NC_015949
Putative virulence/resistance : Resistance
Product : D-alanine--D-alanine ligase
Function : -
COG functional category : M : Cell wall/membrane/envelope biogenesis
COG ID : COG1181
EC number : -
Position : 1920561 - 1921655 bp
Length : 1095 bp
Strand : -
Note : TIGRFAM: D-alanine--D-alanine ligase; HAMAP: D-alanine--D-alanine ligase; KEGG: cki:Calkr_0526 D-alanine/D-alanine ligase; PFAM: D-alanine--D-alanine ligase, C-terminal; D-alanine--D-alanine ligase, N-terminal

DNA sequence :
ATGACCAAGTTAAAGGTTGCTGTTTTGTTTGGAGGAGTTTCAACAGAACACGAAATCTCTATAGTTTCGGCAAAATCAAT
CATGCAAAATATGGACAAGGAAAAATACGAAATAATTCCAATAGGTATAACAAAAGAAGGAAAATGGCTCTTGTACACAG
GTAAAATTGAAGATTTGGATAACAAGTGGACTCAGTTTTCTATAGAATGTTTTATCTCTCCTGATAGAACCAAAAAGGCA
CTTGTAAAAATAAAAGATAACGAGGCTACCTTTATCGACATTGACGTTGTGTTCCCGGTTTTGCATGGACTGAATGGTGA
GGATGGGACAGTTCAGGGACTTTTAGAGTTGTCTAACATACCGTATGTGGGCTGCGGTGTTCTTTCGTCGGCTATATGCA
TGGACAAAGCATTTGCTAAAAAGCTTGCACTTTTAGAAGGAATACCACAGGGTCATTTTTTGGTTGTATACAAAAACGAA
TATTCAGCTAAAAAAGATTATTTTATAAGGCGAATAGAGAGTGAATTTTCGTATCCTGTTTTTGTAAAGCCTGCAAACTC
AGGCTCATCTGTTGGTATTTCAAAAGCAAAAGACAGAGAAGACCTTGTTTTAGCAATACATGAGGCTTTTTTGTATGATA
CAAAGATTTTGATTGAACAGGCTATCAACGCTCGTGAGATAGAATGCGCAGTTCTTGGGAATGATGAGGTATTTGTCTCT
GAACCTGGAGAAATAATTCCGTCAAGAGAGTTTTATTCGTATGAGGCAAAGTATATTGATAATTCATCAGAGCTCATCAT
CCCGGCAAGACTTCCAAAAGAAGTTGTTGAAGAGATAAAAGATTTGGCAGCAAGGATTTACAAAATTTTTGAGTGCTGTG
GAATGGCAAGAGTGGACTTTTTTGTTGATAAAGATACGAACAAAGTGTATTTCAACGAGGTAAACACAATACCTGGCTTT
ACAAGTATTTCGATGTATCCAAAACTTATGGAGTTCAGTAGCATTCCATATTCTCAGCTCATTGATAAACTAATTTCTCT
TGCCATTGAGAAGAATGAGCAGAAAAAAAGCATAAAATACAGCAAAGAGGGCTGA

Protein sequence :
MTKLKVAVLFGGVSTEHEISIVSAKSIMQNMDKEKYEIIPIGITKEGKWLLYTGKIEDLDNKWTQFSIECFISPDRTKKA
LVKIKDNEATFIDIDVVFPVLHGLNGEDGTVQGLLELSNIPYVGCGVLSSAICMDKAFAKKLALLEGIPQGHFLVVYKNE
YSAKKDYFIRRIESEFSYPVFVKPANSGSSVGISKAKDREDLVLAIHEAFLYDTKILIEQAINAREIECAVLGNDEVFVS
EPGEIIPSREFYSYEAKYIDNSSELIIPARLPKEVVEEIKDLAARIYKIFECCGMARVDFFVDKDTNKVYFNEVNTIPGF
TSISMYPKLMEFSSIPYSQLIDKLISLAIEKNEQKKSIKYSKEG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
vanB NP_815951.1 D-alanine--D-lactate ligase Not tested Not named Protein 2e-59 44
vanB YP_008394259.1 Vancomycin B-type resistance protein vanB, D-alanine--D-lactate ligase Not tested Not named Protein 2e-58 43

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Calla_1828 YP_004799387.1 D-alanine--D-alanine ligase U00456.gene.p01 Protein 1e-59 44
Calla_1828 YP_004799387.1 D-alanine--D-alanine ligase U35369.1.gene6.p01 Protein 1e-59 44
Calla_1828 YP_004799387.1 D-alanine--D-alanine ligase AE016830.1.gene2250. Protein 1e-59 44
Calla_1828 YP_004799387.1 D-alanine--D-alanine ligase NC_005054.2598280.p0 Protein 3e-60 43
Calla_1828 YP_004799387.1 D-alanine--D-alanine ligase NC_014475.1.orf3.gen Protein 3e-60 43
Calla_1828 YP_004799387.1 D-alanine--D-alanine ligase AF130997.1.orf4.gene Protein 1e-57 43
Calla_1828 YP_004799387.1 D-alanine--D-alanine ligase AF277571.gene.p01 Protein 8e-58 43
Calla_1828 YP_004799387.1 D-alanine--D-alanine ligase AF310956.2.orf5.gene Protein 2e-59 43
Calla_1828 YP_004799387.1 D-alanine--D-alanine ligase AY489045.gene.p01 Protein 2e-55 43
Calla_1828 YP_004799387.1 D-alanine--D-alanine ligase AY145441.gene.p01 Protein 2e-59 43