Gene Information

Name : Acife_0787 (Acife_0787)
Accession : YP_004783306.1
Strain : Acidithiobacillus ferrivorans SS3
Genome accession: NC_015942
Putative virulence/resistance : Resistance
Product : mercuric reductase
Function : -
COG functional category : C : Energy production and conversion
COG ID : COG1249
EC number : -
Position : 799221 - 800879 bp
Length : 1659 bp
Strand : -
Note : KEGG: tbd:Tbd_1341 putative mercuric reductase; TIGRFAM: Mercuric reductase MerA; PFAM: FAD-dependent pyridine nucleotide-disulphide oxidoreductase; Heavy metal transport/detoxification protein; Pyridine nucleotide-disulphide oxidoreductase, dimerisation

DNA sequence :
ATGGAAAAACATGTTCAATTACAAATAGTCGGGATGACCTGCACGCACTGTGCATTGACCGTAGAAAAAGCCCTTAGGGG
CATCGCTGGTGTCAAAAACGCAAAAGTATCCCTCCCCGAAGGATTGGCGCAGGTAGACGTCGCCAATGATGTTGCCGATT
CCGTACTGACTGCGGCAATAATCAAGAGCGGTTACGGCGTTCAAGCAGCAGGTACTGGCTCAGTTATGCACAGTCAAAAC
GCTGTGGCCAATAACTCCGAGGCAAAGGAGTTGCATGTTGTTATCCTGGGGTCTGGTTCGGGAGCCTTTGCTGCCGCGAT
AAGATGTGTCGAAGGTGGGGCTCGCGTAACGATGATCGAAAAAGGGACCTTGGGGGGAACCTGCGTCAACATTGGCTGTG
TTCCTTCCAAAATCATGATCCGCGCTGCGCAAATGGCACATTGGCAGCGCGCGCACCCATTCCAAGGGCTGGGCCATTGT
GAGCCCACGGTAGAACGCGCTGCACTTGTGGCCCAGCAGCAGAAACGTGTGAGCGAACTGCGCAACGAAAAATACGCCAA
CGTATTAGCTTCCCACCCGGCGATCACCTTTCTGCAGGGAACAGCTCGATTCGCAGATCCCCACACCATTGTCGTGCGCA
CAACTGATGGGGAAGAGCAGTTCGTTCGGGGGGATCGAATCCTCATTGCTGTTGGTGCTCATCCCCAGGTGCCTCGAATA
CCCGGATTGGACCAGACACCCTATTGGACGTCGACAGAAGCACTTGTGGCCGACGAGACACCCCACCACCTTGTTGTATT
AGGTGGTTCGGTGGTGGCTGTAGAGCTTGCACAAGCTTTCCGCAGACTGGGTGCGGAAGTCACCATTCTGGCACGTAGCA
CGTTGTTGTCCAAGGAGGATCCAGCACTGGGTGCGAGTTTGCAGGCCGTATTTGAAGAAGAAGGCATTCGGGTGCTTCAA
CAGACAGTTCCGGAATTCGTGCACCATGAGAATGGGCAGTTTGAACTCCAGATTGCTTCGGAGCTGCTCCGTGCAGATCA
GCTGCTGGTGGCAACGGGCCGTAAACCAAATACTCAGGATTTAGGATTAGAATCCCTTGGAGTCGTGACCAACGAGTTTG
GTGCCATCATGGTTGATGACCACATGCGCACGAGTGTTGCCCACATTTTTGCGACAGGAGACTGTACTACACATCCTCAG
TTCGTTTATGTCGCCGCTGCGGGGGGCACTCGTGCCGCAGTCAATATGTTGGGAGGTGATGCTGCACTGGATCTGAGCGC
CATGCCGTCTGTGGTGTTTACAGATCCTCAAGTAGCCACGGTTGGGATGGACGAAAAAACCGCATTGAGCCAGGGAATAA
GCATCCTCAGTCGTACTCTGAGCCTGGACAATGTGCCCAGAGCCTTGGCGAATTTTGATACCAGGGGATTTGTCAAGCTG
GTAGTGGACGAAACGTCCGGAAAATTGTTGGGGGCGCAGATTCTGGCTGCTGAGGCTGGTGAAATTGTCCAGTCCGCAGC
ATTAGCCTTGCGAGGCGGACTTCGCTTCCAGGATTTGGCGGATCAGTTGTTTCCCTATCTGACCATGGTTGAAGGACTGA
AACTCTGTGCTCAGACTTTTACCAAAGATGTTAAGCAACTGTCCTGTTGTGCCGGGTAA

Protein sequence :
MEKHVQLQIVGMTCTHCALTVEKALRGIAGVKNAKVSLPEGLAQVDVANDVADSVLTAAIIKSGYGVQAAGTGSVMHSQN
AVANNSEAKELHVVILGSGSGAFAAAIRCVEGGARVTMIEKGTLGGTCVNIGCVPSKIMIRAAQMAHWQRAHPFQGLGHC
EPTVERAALVAQQQKRVSELRNEKYANVLASHPAITFLQGTARFADPHTIVVRTTDGEEQFVRGDRILIAVGAHPQVPRI
PGLDQTPYWTSTEALVADETPHHLVVLGGSVVAVELAQAFRRLGAEVTILARSTLLSKEDPALGASLQAVFEEEGIRVLQ
QTVPEFVHHENGQFELQIASELLRADQLLVATGRKPNTQDLGLESLGVVTNEFGAIMVDDHMRTSVAHIFATGDCTTHPQ
FVYVAAAGGTRAAVNMLGGDAALDLSAMPSVVFTDPQVATVGMDEKTALSQGISILSRTLSLDNVPRALANFDTRGFVKL
VVDETSGKLLGAQILAAEAGEIVQSAALALRGGLRFQDLADQLFPYLTMVEGLKLCAQTFTKDVKQLSCCAG

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
merA YP_006098387.1 mercuric ion reductase Not tested Tn2411 Protein 3e-143 58
merA AET25397.1 MerA Not tested PAGI-2(C) Protein 3e-143 58
merA AFG30120.1 MerA Not tested PAGI-2 Protein 3e-143 58
merA AAN62178.1 mercuric (Hg(II)) reductase Not tested PAGI-2(C) Protein 2e-139 57
merA ABQ57371.1 MerA Not tested SGI1 Protein 8e-144 57
merA ACN81005.1 MerA Hg(II) mercuric ion reductase Not tested AbaR5 Protein 5e-141 57
merA AGK07021.1 MerA Not tested SGI1 Protein 8e-144 57
merA AGK07079.1 MerA Not tested SGI1 Protein 8e-144 57
merA CAJ77060.1 Mercuric ion reductase Not tested AbaR1 Protein 3e-141 57
unnamed ABR13401.1 mercuric reductase Not tested PAGI-5 Protein 3e-137 56
merA BAB47642.1 mercuric reductase Not tested Type-III SCCmec Protein 2e-93 41
SE0085 NP_763640.1 dihydrolipoamide dehydrogenase Not tested SCCpbp4 Protein 2e-93 41

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Acife_0787 YP_004783306.1 mercuric reductase BAC0653 Protein 4e-142 56
Acife_0787 YP_004783306.1 mercuric reductase BAC0652 Protein 1e-140 56
Acife_0787 YP_004783306.1 mercuric reductase BAC0650 Protein 6e-134 54
Acife_0787 YP_004783306.1 mercuric reductase BAC0651 Protein 9e-94 41