Gene Information

Name : Acife_0330 (Acife_0330)
Accession : YP_004782885.1
Strain : Acidithiobacillus ferrivorans SS3
Genome accession: NC_015942
Putative virulence/resistance : Unknown
Product : replicative DNA helicase
Function : -
COG functional category : L : Replication, recombination and repair
COG ID : COG0305
EC number : -
Position : 349913 - 351286 bp
Length : 1374 bp
Strand : +
Note : TIGRFAM: DNA helicase, DnaB type; PFAM: DNA helicase, DnaB-like, C-terminal; DNA helicase, DnaB-like, N-terminal; KEGG: afr:AFE_2819 replicative DNA helicase; SMART: ATPase, AAA+ type, core

DNA sequence :
GTGTTCGAGTCGGCGGACTATGGCGGCGGGGTCAAGGCCCCGCCGCACTCTATTGAGGCAGAGCAATCGGTCATCGGTGC
CCTGCTGATTGATCCCGATACAGCGGATCAAGTGACCGAGGCGGTGACGGCCGAAGCTTTTTATGAGCGCCGTCATCGCC
AGATTTTTCAAGCGATCACTGCCATGCTGGGTGCCGGGCGGGCAGTAGACGCCGTCACGGTCTCCGAGTGGTTGCAAGAT
CACGAGCAGCTTGCCGATGTGGGCGGGGTGCAATATCTTCTCATGCTCGCCAACGAGACACCCTCGGCGGCCAATGTGCT
CGCCTATGCGAGCATCGTGCGGGAGCGGGCCACTCTGCGTGATCTTATCCGGGTTGGCCGGGAAATTGCCGAGCTGGCCT
ATCGTCCGGAAGGGCGAGAAGCGCAGGCCCTGTTGGACGAGGCGGAAAGCCGGGTTTTTCAGCTCGCCGAAGGACAGCAG
CGGGCCGGAGAGGGCTTCCGGCAACTCAAGGATGCACTTCCGGCGGTAATCGACCGGATCGAGACCATCGCCCGCGAGAA
AAAAGGCGTCACCGGCCTGTCCACAGGCTTTGCCGATCTGGACGAGAAAACCTCCGGCCTGCATCCGGGTCAGCTCATCA
TCGTTGCTGGTCGTCCTAGTATGGGCAAAACCTCTTTCGCCATGAATATAGCTGAGCATGTCGCCTGTAACGAAGGCAAG
CCGGTGGCGGTTTTCAGCATGGAAATGCCGATAGACGAAATCGTCTTGCGCGCACTCTCGTCGCGAGGACGGGTGGACCA
GCACCGGCTGCGCAATGGTAGCCTGGGTAATGATGACTGGCCGCGCATCGCGCATGCCATTGGCGAACTCGGCAATGCGC
CGTTGTTTGCCGATGATTCGGCAGCGCTGACGCCGACGGATTTGCGTTCGCGGGCGCGTCGGCTGAAGCGAGAGCATGGC
TTGGCGCTGGTTGTGGTCGACTATCTGCAACTCATGCAGGTGCCGGGGCGGGGTGACAATCGGGTCGCTGAGATTTCCGA
GATCAGCCGTTCGCTCAAGGCCTTGGCCAAAGAGCTGTCCATCCCAGTGATTGCCCTCTCGCAGCTCAATCGTAGCCTGG
AAAACCGGACCGAAAAGCGTCCGCAGATGTCGGATCTGCGTGAATCCGGTGCTATTGAACAGGACGCCGATCTGATTCTG
TTCCTGTACCGCGATGAGGTGTATTACAAGGACAAGGAAGAGGTGAAAGGCATTGCCGAGGTGATTATCGGCAAGCAGCG
TAACGGACCCATCGGGACCGTGCGGATGAGCTTCTTCGGTGAATATACCCGCTTTGAGAATTATGCGCCGTCCGGGGATA
GTTTCGGGCATTAG

Protein sequence :
MFESADYGGGVKAPPHSIEAEQSVIGALLIDPDTADQVTEAVTAEAFYERRHRQIFQAITAMLGAGRAVDAVTVSEWLQD
HEQLADVGGVQYLLMLANETPSAANVLAYASIVRERATLRDLIRVGREIAELAYRPEGREAQALLDEAESRVFQLAEGQQ
RAGEGFRQLKDALPAVIDRIETIAREKKGVTGLSTGFADLDEKTSGLHPGQLIIVAGRPSMGKTSFAMNIAEHVACNEGK
PVAVFSMEMPIDEIVLRALSSRGRVDQHRLRNGSLGNDDWPRIAHAIGELGNAPLFADDSAALTPTDLRSRARRLKREHG
LALVVVDYLQLMQVPGRGDNRVAEISEISRSLKALAKELSIPVIALSQLNRSLENRTEKRPQMSDLRESGAIEQDADLIL
FLYRDEVYYKDKEEVKGIAEVIIGKQRNGPIGTVRMSFFGEYTRFENYAPSGDSFGH

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
unnamed ABR13456.1 replicative DNA helicase Not tested PAGI-5 Protein 1e-92 48
PMI2640 YP_002152342.1 DNA helicase Not tested Not named Protein 2e-83 47
dnaB CAI36044.1 replicative DNA helicase DnaB Not tested PPHGI-1 Protein 6e-77 46
dnaB2 YP_001402701.1 replicative DNA helicase Not tested YAPI Protein 7e-89 46
api2 CAF28476.1 putative DNA binding protein with DNA-dependent ATPase activity Not tested YAPI Protein 2e-88 45
dnaB2 YP_001007676.1 putative DNA helicase Not tested YAPI Protein 3e-84 45
STY4522 NP_458617.1 DNA helicase Not tested SPI-7 Protein 2e-83 45
t4227 NP_807829.1 DNA helicase Not tested SPI-7 Protein 2e-83 45
dnaB1 YP_005176258.1 replicative DNA helicase Not tested ICEPmu1 Protein 4e-72 42