Gene Information

Name : SPPN_01485 (SPPN_01485)
Accession : YP_004767601.1
Strain : Streptococcus pseudopneumoniae IS7493
Genome accession: NC_015875
Putative virulence/resistance : Resistance
Product : erythromycin ribosome methylase
Function : -
COG functional category : J : Translation, ribosomal structure and biogenesis
COG ID : COG0030
EC number : -
Position : 239535 - 240272 bp
Length : 738 bp
Strand : +
Note : COG0030 Dimethyladenosine transferase (rRNA methylation)

DNA sequence :

ATGAACAAAAATATAAAATATTCTCAAAACTTTTTAACGAGTGAAAAAGTACTCAACCAAATAATAAAACAATTGAATTT
AAAAGAAACCGATACCGTTTACGAAATTGGAACAGGTAAAGGGCATTTAACGACGAAACTGGCTAAAATAAGTAAACAGG
TAACGTCTATTGAATTAGACAGTCATCTATTCAACTTATCGTCAGAAAAATTAAAACTGAATACTCGTGTCACTTTAATT
CACCAAGATATTCTACAGTTTCAATTCCCTAACAAACAGAGGTATAAAATTGTTGGGAATATTCCTTACCATTTAAGCAC
ACAAATTATTAAAAAAGTGGTTTTTGAAAGCCGTGCGTCTGACATCTATCTGATTGTTGAAGAAGGATTCTACAAGCGTA
CCTTGGATATTCACCGAACACTAGGGTTGCTCTTGCACACTCAAGTCTCGATTCAGCAATTGCTTAAGCTGCCAGCGGAA
TGCTTTCATCCTAAACCAAAAGTAAACAGTGTCTTAATAAAACTTACCCGCCATACCACAGATGTTCCAGATAAATATTG
GAAGCTATATACGTACTTTGTTTCAAAATGGGTCAATCGAGAATATCGTCAACTGTTTACTAAAAATCAGTTTCATCAAG
CAATGAAACACGCCAAAGTAAACAATTTAAGTACCGTTACTTATGAGCAAGTATTGTCTATTTTTAATAGTTATCTATTA
TTTAACGGGAGGAAATAA

Protein sequence :

MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTTKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLI
HQDILQFQFPNKQRYKIVGNIPYHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNNLSTVTYEQVLSIFNSYLL
FNGRK

• Homologs from PAI DB

Gene	GenBank Accn	Product	Virulance or Resistance	PAI or REI	Alignment Type	E-val	Identity
ermA	NP_373288.1	rRNA methylase	Not tested	Type-II SCCmec	Protein	8e-54	52
ermA-3	YP_190052.1	rRNA adenine N-6-methyltransferase	Not tested	Type-II SCCmec	Protein	8e-54	52
unnamed	BAB47663.1	rRNA adenine N-6-methyltransferase	Not tested	Type-III SCCmec	Protein	2e-53	52
unnamed	BAC53825.1	rRNA adenine N-6-methyltransferase	Not tested	SRImec-III and SCCmec-III region	Protein	2e-53	52
ermA	BAC57474.1	rRNA methylase	Not tested	Type-IIIinv SCCmec	Protein	6e-54	52
ermA	BAA82205.1	rRNA adenine N-6-methyltransferase	Not tested	Type-II SCCmec	Protein	6e-54	52
ermA1	YP_039522.1	rRNA adenine N-6-methyltransferase 1	Not tested	Type-II SCCmec	Protein	8e-54	52
ermA	NP_370576.1	rRNA methylase	Not tested	Type-II SCCmec	Protein	8e-54	52
ermC	NP_273130.1	rRNA adenine N-6-methyltransferase	Not tested	IHT-A	Protein	3e-57	51

• Homologs from CARD and BacMet (resistance genes)

Gene	GenBank Accn	Product	ID of source DB	Alignment Type	E-val	Identity
SPPN_01485	YP_004767601.1	erythromycin ribosome methylase	AM410044.gene14.p01	Protein	1e-111	99
SPPN_01485	YP_004767601.1	erythromycin ribosome methylase	AM903082.gene3.p01	Protein	1e-111	99
SPPN_01485	YP_004767601.1	erythromycin ribosome methylase	AM180355.1.gene2254.	Protein	8e-111	98
SPPN_01485	YP_004767601.1	erythromycin ribosome methylase	AM180355.1.gene2259.	Protein	8e-111	98
SPPN_01485	YP_004767601.1	erythromycin ribosome methylase	NC_009782.5559087.p0	Protein	4e-54	52
SPPN_01485	YP_004767601.1	erythromycin ribosome methylase	NC_002758.1121630.p0	Protein	4e-54	52
SPPN_01485	YP_004767601.1	erythromycin ribosome methylase	NC_002952.2859615.p0	Protein	4e-54	52
SPPN_01485	YP_004767601.1	erythromycin ribosome methylase	NC_002745.1123579.p0	Protein	4e-54	52
SPPN_01485	YP_004767601.1	erythromycin ribosome methylase	NC_002745.1124852.p0	Protein	4e-54	52
SPPN_01485	YP_004767601.1	erythromycin ribosome methylase	NC_009782.5559229.p0	Protein	4e-54	52
SPPN_01485	YP_004767601.1	erythromycin ribosome methylase	NC_002758.1120012.p0	Protein	4e-54	52
SPPN_01485	YP_004767601.1	erythromycin ribosome methylase	NC_002952.2859614.p0	Protein	4e-54	52
SPPN_01485	YP_004767601.1	erythromycin ribosome methylase	NC_002745.1122822.p0	Protein	4e-54	52
SPPN_01485	YP_004767601.1	erythromycin ribosome methylase	NC_002745.1124324.p0	Protein	4e-54	52
SPPN_01485	YP_004767601.1	erythromycin ribosome methylase	NC_002745.1125313.p0	Protein	4e-54	52
SPPN_01485	YP_004767601.1	erythromycin ribosome methylase	AJ313523.1.orf0.gene	Protein	8e-54	52
SPPN_01485	YP_004767601.1	erythromycin ribosome methylase	V01278.gene.p01	Protein	2e-58	52
SPPN_01485	YP_004767601.1	erythromycin ribosome methylase	M64090.gene.p01	Protein	3e-56	52
SPPN_01485	YP_004767601.1	erythromycin ribosome methylase	M15332.gene.p01	Protein	2e-53	51
SPPN_01485	YP_004767601.1	erythromycin ribosome methylase	NC_010686.6295746.p0	Protein	1e-57	51
SPPN_01485	YP_004767601.1	erythromycin ribosome methylase	NC_010685.6295744.p0	Protein	1e-57	51
SPPN_01485	YP_004767601.1	erythromycin ribosome methylase	NC_007792.3912746.p0	Protein	5e-57	50
SPPN_01485	YP_004767601.1	erythromycin ribosome methylase	NC_007209.3523503.p0	Protein	1e-57	50