Gene Information

Name : MELS_0629 (MELS_0629)
Accession : YP_004765678.1
Strain : Megasphaera elsdenii DSM 20460
Genome accession: NC_015873
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : -
Position : 729573 - 731321 bp
Length : 1749 bp
Strand : +
Note : -

DNA sequence :
ATGCTGAAAAAAACAAAGATCGTCTGCACCTTAGGCCCCAGTTCCAATACCCCGGAAATTATCGAAAAGATGATTAAAGC
CGGCATGAACGTTGCCCGCTTTAATTTCTCCCATGGTTCTCATGAAGAACATAAACAGCGCATCGACATGGTCCGCGCTG
CCTCCCAGAAACTGGGTATCCCTGTGGCACTGCTCCTGGACACGAAAGGCCCGGAAATCCGTTTGGGCAAATTTAAGAAT
GGCTCGATCATGATGGAAGCCGGCAAGGACTTTACCTTGACGGCCCGTGACATCGAAGGCGACGAAACGATTGCCTCCAT
GAACTATAAAGAACTGCCGCAGGACGTCAAAGCCGGCGACCACATCCTCTTGTCGGACGGCCTGGTCAACTTGGAAGTTG
TCAGCGTCGACGGCGAAGATATCCATACGAAGATTCTCAACAGCGGCAAGATGAGCGACCGCAAGCGCGTTGCCGTACCG
GGCATTGCCATCAACCTGCCGGCCGTTTCTGAAACCGATGCCGCTGACATCGAATTTGGCTGCCGCATGAATATGGACTG
GATTGCCGCTTCTTTCATCCAGCGCGGCAAAGATGTCCTGACGATCCGCAAGATTTTGGAAAAACACGACAGCCACATGA
AGATTATCTCCAAAATCGAATGTCAGGCAGCTGTCAACAATATCGACGATATCATTAAGATGAGCGACGGCATCATGGTT
GCCCGTGGTGACCTCGGCGTCGAAGTCCCGGCGGAAGAAGTACCGATGCTCCAGAAAGTCCTCATCCATAAATGCCAGGC
TGCGGGCAAACCGGTCATTACGGCGACGCAGATGCTGGAATCGATGTGCAACAACCCGCGTCCGACCCGGGCAGAAACGA
GCGACGTTGCTAACGCCATCCTCGATGGCACCGATGCCATCATGCTCAGCGGCGAAACGGCTGGTGGCCTCTATCCGGTA
GAAGCCGTAGAAACTATGGCCCGCGTCGCAACCTTTACGGAAAGCAACTTCACGCCGAAGTCCTATGAAGATGTCGACGC
CGAATCGACAACGACGACGGAATCCATCGGCAAAGCCGTCGTCAAGATTGCCAAAGACCTCCACGCTGCGGCTATCATCG
CTTCGACCGAACGCGGCGGCACAGCGCAGATGATTTCTAAATTCCGCCCGGCCTGCCCGATTGTGGCCGTATCGCCGCAC
GAAGCCATCGTCCGCCGCTTGCAGCTCAACTGGGGCGTCCAGGCCGTCACGGGCAAAGAAGCGAACGACACCGACGCTGT
CGTCGATAATGCTATCTTCGCAGCCCTCGACAACGACCTCATCAAAGCCGGCGACCTCGTCGTCCTCACGGCCGGTGTCC
CCGTTGCCAAAGCCGGTTCGACCAACATGATCCGCGTCCAGGTCGTCGGTGACGTCCTCCTCCGCGGTACCGGTATCGGC
CGTAATTCGGCCATCGGCAAAGTCTGTGTCGCTGAATCGAAAGAAGACCTGGAAAAGAACTTCAGCGACGGCTGCATCCT
CGTCCTCCGTTCGGCCCGCGCTGATTTCGTCGATTATATGAAACGGGCCAGCGCCATCGTCTCCGAAGAAGTCGGCCTGA
CCAGTGAATCAGCCATCGTCGCCATCACCTTGGGCATCCCGACCGTCGTCGGCGCAGCTCATGCCGTAGACACTCTGGAA
AATGGCGAAATCGTCACCGTAGACGCCAGCCGCGGCACCATCTTCCGCGGCGAAGCCAACGCACGATAA

Protein sequence :
MLKKTKIVCTLGPSSNTPEIIEKMIKAGMNVARFNFSHGSHEEHKQRIDMVRAASQKLGIPVALLLDTKGPEIRLGKFKN
GSIMMEAGKDFTLTARDIEGDETIASMNYKELPQDVKAGDHILLSDGLVNLEVVSVDGEDIHTKILNSGKMSDRKRVAVP
GIAINLPAVSETDAADIEFGCRMNMDWIAASFIQRGKDVLTIRKILEKHDSHMKIISKIECQAAVNNIDDIIKMSDGIMV
ARGDLGVEVPAEEVPMLQKVLIHKCQAAGKPVITATQMLESMCNNPRPTRAETSDVANAILDGTDAIMLSGETAGGLYPV
EAVETMARVATFTESNFTPKSYEDVDAESTTTTESIGKAVVKIAKDLHAAAIIASTERGGTAQMISKFRPACPIVAVSPH
EAIVRRLQLNWGVQAVTGKEANDTDAVVDNAIFAALDNDLIKAGDLVVLTAGVPVAKAGSTNMIRVQVVGDVLLRGTGIG
RNSAIGKVCVAESKEDLEKNFSDGCILVLRSARADFVDYMKRASAIVSEEVGLTSESAIVAITLGIPTVVGAAHAVDTLE
NGEIVTVDASRGTIFRGEANAR

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 2e-91 46
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 2e-91 46
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 9e-91 46
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 2e-91 46

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
MELS_0629 YP_004765678.1 pyruvate kinase VFG0479 Protein 7e-92 46