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Name : pykA (zobellia_1594) Accession : YP_004736037.1 Strain : Zobellia galactanivorans DsiJT Genome accession: NC_015844 Putative virulence/resistance : Virulence Product : pyruvate kinase Function : - COG functional category : G : Carbohydrate transport and metabolism COG ID : COG0469 EC number : 2.7.1.40 Position : 1890751 - 1892181 bp Length : 1431 bp Strand : + Note : Pyruvate kinase (PK) catalyzes the final step in glycolysis (Embden-Meyerhof pathway), the conversion of phosphoenolpyruvate to pyruvate with concomitant phosphorylation of ADP to ATP. PK forms a homotetramer and requires both magnesium and potassium ions DNA sequence : ATGCCAAGTAACAAAAAGACAAAAATCGTTGCCACCCTAGGGCCGGCCACGAGCAAAAAGGAAGTGCTGAGAGATATGAT GGAAGCCGGCGTAGATGTCTTTCGCATTAATTTTTCACATGCCGATTATAACGACGTTACCGAACGTATAAAAATGATTA GGGAACTCAATGAAGAAACAGGTAGAAACACCTCTATTCTGGCCGATTTACAAGGCCCGAAACTTAGGGTCGGTGTTATG GCCAGTGAAGTCGTTGTAGCCCCTGGAGACGAAATTACCTTTGTTACGGGCGAACCTTTTGAAGGTACCGCCGAAAGGGT ATATATGAACTATGATGCTTTTCCTCAAGACGTCAAGCCCGGTGAGCGTATTTTGTTAGACGACGGAAAGTTGATGTTCG AAGTCATAACCACTGACGGAAAGGAGCAAGTAAAAACCAAGGTTATACAAGGAGGACCTTTGCGGTCTAAAAAAGGCGTA AACCTTCCCAATACAAATATCTCTTTACCCGCCTTGACCGAAAAAGACGTAAAAGATGCCATTTTTGCCATTTCGCAAAA TGTCGATTGGATCGCGCTTTCCTTCGTCCGTTTTAGCCAAGACCTTATTGATCTTCAAGCGATCATTAAAGAACATACAG ACGTAAAAATTCCGATCATCGCGAAAATCGAAAAACCGGAAGCCGTTGAAAACATAGACAAAATCGTTGCCTATTGCGAC GGACTAATGGTAGCCCGTGGCGACTTAGGTGTTGAAGTTCCTGCCCAAGAGGTTCCATTAATTCAGAAACAATTGGTGCT TAGGGCCAAAAAAGCCCGTATCCCCGTAATAATCGCCACCCAAATGATGGAAACGATGATTACCAGCCTTACACCGACAC GTGCAGAGGTGAACGATGTAGCCAACTCCGTAATGGACGGTGCCGATGCCGTGATGCTCTCTGGGGAAACCTCAGTGGGG AATTACCCGGTACAGGTTATCGAAAAAATGAGCAGTATCCTTCAAAGTGTAGAAAGCTCTGAACTTATTCAAGTACCACA CGATCCACCACATATCCGTACAAAAAGATATATTACAAAGTCGATTTGTTACCATGCCGCAATTATGGCCAATGAAATCA AGGCCAAGGCCATCTCTACATTGACCAATAGTGGGTATACGGCCTTTCAAATCTCGGCTTGGCGACCTAGTGCACACATT TTGGTGTTTACCTCAAACAAACGTATACTTACGCAATTGAACCTTTTATGGGGCGTGCGTGCCTTTTATTATGACAAATA CGTATCTACCGATCAAACGATCGAAGACGTGAACAAAATGGCATGTGATAAAGGCCTTTTAGAAACTGGAGATATGCTTA TCAGTTTGGCGGCAATGCCGATCAAAGACAAAGGTATGGTCAACACGCTAAGGGTGACAGAAATAGAATAA Protein sequence : MPSNKKTKIVATLGPATSKKEVLRDMMEAGVDVFRINFSHADYNDVTERIKMIRELNEETGRNTSILADLQGPKLRVGVM ASEVVVAPGDEITFVTGEPFEGTAERVYMNYDAFPQDVKPGERILLDDGKLMFEVITTDGKEQVKTKVIQGGPLRSKKGV NLPNTNISLPALTEKDVKDAIFAISQNVDWIALSFVRFSQDLIDLQAIIKEHTDVKIPIIAKIEKPEAVENIDKIVAYCD GLMVARGDLGVEVPAQEVPLIQKQLVLRAKKARIPVIIATQMMETMITSLTPTRAEVNDVANSVMDGADAVMLSGETSVG NYPVQVIEKMSSILQSVESSELIQVPHDPPHIRTKRYITKSICYHAAIMANEIKAKAISTLTNSGYTAFQISAWRPSAHI LVFTSNKRILTQLNLLWGVRAFYYDKYVSTDQTIEDVNKMACDKGLLETGDMLISLAAMPIKDKGMVNTLRVTEIE |
| Gene | GenBank Accn | Product | Virulance or Resistance | PAI or REI | Alignment Type | E-val | Identity |
| STY1744 | NP_456147.1 | pyruvate kinase | Not tested | SPI-2 | Protein | 6e-65 | 41 |
| pykF | CAA68205.1 | pyruvate kinase like protein | Not tested | SPI-2 | Protein | 4e-64 | 41 |
| pykF | YP_216386.1 | pyruvate kinase | Not tested | SPI-2 | Protein | 3e-64 | 41 |
| pykF | NP_805051.1 | pyruvate kinase | Not tested | SPI-2 | Protein | 3e-64 | 41 |
| Gene | GenBank Accn | Product | ID of source DB | Alignment Type | E-val | Identity |
| pykA | YP_004736037.1 | pyruvate kinase | VFG0479 | Protein | 1e-64 | 41 |
