Gene Information

Name : pykF (MEPCIT_131)
Accession : YP_004706657.1
Strain : Candidatus Moranella endobia PCIT
Genome accession: NC_015735
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : -
Position : 154629 - 156071 bp
Length : 1443 bp
Strand : +
Note : predicted based on similarity to TIGRFAM pyruv_kin and related proteins in nr

DNA sequence :
ATGTCCAGACAGCTAAGAAGAACAAAAATTGTCACTACTCTCGGTCCGGTAACCGATCGTGATAATAATCTTGAAAAAGT
AATAAACGCCGGAGCCAACGTTGTTCGCCTCAATTTTTCACACGGTAGCGCAGAAGAGCATATCCTAAGGGCACAAAAAG
TGCGCGATATTTCAGCCCGCCTCGGCCGACATATCGCGGTACTTGGTGATTTACAGGGGCCAAAAATACGCATTTCAACT
TTTAGGGACGGGAACATTGTCCTGAAAGTAAGCGACAAATTTTTACTAGATGCTTCATTAGGCCGCGGTGAAGGTGACTG
CGAACGTGTAGGCATTGACTATAAAGGTTTACCGGAAGATGTGGCGCCAGGCGATATACTGCTACTAGATGATGGCCGAG
TACAACTGAAAGTGCTCGAAGTTCAGGGTATCAGGGTTTATACCCAAGTCACGGTAGGTGGGCCGCTGTCCAACAATAAA
GGTATAAACAAGCTTGGAGGTGGTTTGTCTGCTGAAGCTCTGACTGAGAAAGACAAATCAGATATCATTACTGCAGCAAA
AATAGGTGTTGATTACCTGGCGATTTCATTTCCCCGCACTAGTGAAGATCTTAACTATGCGCGCCGGCTGGCGCGTGAGG
CCGGCAGCTATGCCAAAATTGTATCAAAAATAGAAAGAGCAGAAGCTGTGGCTACCGAGGAAGCCATGGACGAAATCATT
CTCGCTTCAGATGTGGTGATGGTGGCAAGGGGCGACTTAGGTGTGGAAATCGGCGATCCAGAATTAGTAGGTATTCAAAA
AACATTAATCCGCCGAGCCCGCACTCTTAACCGTGCCGTTATTACTGCTACGCAGATGATGGAATCAATGATTAATAACC
CGATGCCCACGCGCGCTGAAGTGATGGATGTTGCTAATGCGGTGCTAGACGGCACCGACGCGGTAATGCTGTCAGCCGAA
ACGGCAGCAGGGCGGTATCCAGCCGAAACAGTATCTACTATGGCAAGCGTTTGCCTTGGAGCGGAAAAAATTCCCAGTAT
TAATCTCTCCAAGCATCGGTTGGACGTGCAATTTGACAATATTGAAGAAGCCATAGCTATGGCTGCTATGTATGCTGCTA
ATCATCTTAAGGGTGTAACAGCAATTATCTCAATGACGGAATCTGGCAGCACCGCACTCATGATGTCCAGGATTAGTTCT
GGTCTACCAATTTTTGCTCTATCGCGACACGAACATACCCTAAATCTTACCGCTCTTTATCGCGGCGTAACACCAGTATA
CTTTGACGGCGATAGTGAAGGCGTGGCAGCAGCAATTAATGCTACTAATTTACTGAGAGATAAAGGTTTCTTGAAATCTG
GTGATCTAGTCATCATCACCCAGGGGGATGTAATGGATCTAGTAGGTACTACAAACACCAGTCGTATTCTGCGCGTCAAG
TAA

Protein sequence :
MSRQLRRTKIVTTLGPVTDRDNNLEKVINAGANVVRLNFSHGSAEEHILRAQKVRDISARLGRHIAVLGDLQGPKIRIST
FRDGNIVLKVSDKFLLDASLGRGEGDCERVGIDYKGLPEDVAPGDILLLDDGRVQLKVLEVQGIRVYTQVTVGGPLSNNK
GINKLGGGLSAEALTEKDKSDIITAAKIGVDYLAISFPRTSEDLNYARRLAREAGSYAKIVSKIERAEAVATEEAMDEII
LASDVVMVARGDLGVEIGDPELVGIQKTLIRRARTLNRAVITATQMMESMINNPMPTRAEVMDVANAVLDGTDAVMLSAE
TAAGRYPAETVSTMASVCLGAEKIPSINLSKHRLDVQFDNIEEAIAMAAMYAANHLKGVTAIISMTESGSTALMMSRISS
GLPIFALSRHEHTLNLTALYRGVTPVYFDGDSEGVAAAINATNLLRDKGFLKSGDLVIITQGDVMDLVGTTNTSRILRVK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pykA NP_273151.1 pyruvate kinase Not tested IHT-A Protein 2e-80 50
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 3e-53 44
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 3e-53 44
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 3e-53 44
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 6e-53 43

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
pykF YP_004706657.1 pyruvate kinase VFG0479 Protein 1e-53 44