Gene Information

Name : yscN (HYPMC_3472)
Accession : YP_004677249.1
Strain : Hyphomicrobium sp. MC1
Genome accession: NC_015717
Putative virulence/resistance : Virulence
Product : ATP synthase yscN
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : 3.6.3.14
Position : 3328132 - 3329478 bp
Length : 1347 bp
Strand : -
Note : Evidence 3 : Function proposed based on presence of conserved amino acid motif, structural feature or limited homology

DNA sequence :
ATGGATGCCCTGGTCCGGAAGAGTGGGGAATTCGAATACGTCTTTCGCATCGCCTCTCAGGCCATTGCCGAGGCCACGCC
GATCACTGTGCGCGGACGTGTGCTCCAAGTGTTGGGCACGATCATCAAGGCTACAGTACCTGGAGCGCGCATTGGCGATC
TCTGCATCCTGCACAATCCTTCAGACGGCTCTGAGCTGCCGGCGGAAGTGGTGGGCCTGACGCGCGACTCAGCGATCTTG
ACGCCTCTCGGCGACCTTGGTGGCGTCTCAACGCTGACCGAGGTGCTTCCCACTGGCAAGCCTCATATGGCGCCGGCGGG
CCGTGCGTTGCTGGGCCGAGTGCTGGACGGGATGGGGCAGCCGCTCGATATCGCGGAGCGAGGACCGCTGCGGCCGGATG
CCTATGTTTCAGTCTACGCTGACGCGCCGGACCCACTTACGCGGCGGGTGATCGATACGCCAATGCCGCTTGGTGTGCGC
GCTCTGGATGGCATGCTGACCTGCGGAGAGGGCCAGCGGATGGGCATCTTCGCTGCGGCTGGCGGCGGCAAGTCGACACT
GATGTCTATGCTGGTGCGTGGCGCGGCAGTCGACGTCACGGTTATGGCGCTGATCGGCGAGCGTGGGCGCGAAGTGCGCG
AATTCATCGAGCGCGATCTGGGACCTGAAGGGCGCGAGAAGTCGGTGGTAGTGGTCGCGACCTCGGACCGCTCTTCAATG
GAGCGCGCCAAGGCGGCCTACGTCGCGACTGCTATTGCCGAATGGTTCCGAAATCAGGGTATGCGCGTGTTGTTCCTTAT
GGACAGCGTTACTCGCTTTGCGCGTGCGCAACGAGAGATCGGCTTGGCTGCCGGCGAGCCGCCAACGAGGCGCGGTTTCC
CTCCCTCGGTCTTTGCCACGTTGCCCAAGCTGATGGAGCGCGTCGGTAATAACGACAAAGGCTCGATCACGGCCTTCTAT
ACCGTCCTGGTTGAAGGTGATGACATGTCCGAACCAATCGCGGATGAGACGCGCTCCATCCTGGACGGACATATTGTGCT
GTCACGTGCGCTTTCGGCCGCTAATCACTTTCCCGCGATCGACGTGCTAGCTAGTGCCAGCCGCGTAATGGGGGCTATCG
CCAGCAGCGAGCATCGTGCCCTTGCCGGGCGTATCCGTACGCTGATGGCAAAGTACAATGAGGTCGAGCTGCTGGTGCGT
ATCGGCGAATACAAGCCAGGCAGTGACAAGGTGGCGGATGAGGCCGTCAAAAAGATCGAGCACATCCGAAGCTTCCTCAA
GCAGAATGCGCAAGATCCAGTGGGTTTTAACGATGTGCTGAAGCAGATGGCGGAGGCGCTGGCATGA

Protein sequence :
MDALVRKSGEFEYVFRIASQAIAEATPITVRGRVLQVLGTIIKATVPGARIGDLCILHNPSDGSELPAEVVGLTRDSAIL
TPLGDLGGVSTLTEVLPTGKPHMAPAGRALLGRVLDGMGQPLDIAERGPLRPDAYVSVYADAPDPLTRRVIDTPMPLGVR
ALDGMLTCGEGQRMGIFAAAGGGKSTLMSMLVRGAAVDVTVMALIGERGREVREFIERDLGPEGREKSVVVVATSDRSSM
ERAKAAYVATAIAEWFRNQGMRVLFLMDSVTRFARAQREIGLAAGEPPTRRGFPPSVFATLPKLMERVGNNDKGSITAFY
TVLVEGDDMSEPIADETRSILDGHIVLSRALSAANHFPAIDVLASASRVMGAIASSEHRALAGRIRTLMAKYNEVELLVR
IGEYKPGSDKVADEAVKKIEHIRSFLKQNAQDPVGFNDVLKQMAEALA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
lscN AAO18044.1 LscN Virulence TTSS locus Protein 2e-131 65
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 3e-116 60
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 3e-116 60
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-116 60
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 8e-115 60
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-115 60
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 6e-115 60
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 5e-116 60
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-115 59
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 2e-115 59
escN YP_003236088.1 translocator EscN Virulence LEE Protein 1e-93 52
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 2e-95 51
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-95 51
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 1e-95 51
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 1e-95 51
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 7e-97 51
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 2e-95 51
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 2e-95 51
unnamed AAL06369.1 EscN Virulence LEE Protein 1e-92 51
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 8e-95 51
escN CAC81862.1 EscN protein Virulence LEE II Protein 3e-93 51
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 3e-93 51
escN AAK26715.1 EscN Virulence LEE Protein 2e-93 51
escN AAL57542.1 EscN Virulence LEE Protein 2e-93 51
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 6e-94 51
escN NP_290268.1 hypothetical protein Virulence LEE Protein 9e-94 51
escN CAI43874.1 EscN protein Virulence LEE Protein 3e-93 51
ECs4568 NP_312595.1 EscN Virulence LEE Protein 9e-94 51
escN AAC38383.1 EscN Virulence LEE Protein 7e-94 51
escN AAC31513.1 L0034 Virulence LEE Protein 6e-94 51
escN YP_003223475.1 translocator EscN Virulence LEE Protein 3e-93 51
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 4e-86 50
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 9e-96 50
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 5e-83 50
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 1e-89 50
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 1e-89 50
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 4e-93 49
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 2e-92 49
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 2e-87 49
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 8e-93 48
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 2e-82 47
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 2e-82 47
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 3e-95 47
invC AAS66861.1 InvC Not tested SSR-2 Protein 3e-81 46
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 2e-82 45
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 1e-82 45
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 1e-82 45
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 1e-82 45
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 1e-82 45
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 7e-78 43

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
yscN YP_004677249.1 ATP synthase yscN VFG0048 Protein 4e-153 69
yscN YP_004677249.1 ATP synthase yscN VFG0390 Protein 1e-132 65
yscN YP_004677249.1 ATP synthase yscN VFG0192 Protein 6e-132 65
yscN YP_004677249.1 ATP synthase yscN VFG2144 Protein 2e-113 55
yscN YP_004677249.1 ATP synthase yscN VFG0515 Protein 1e-95 51
yscN YP_004677249.1 ATP synthase yscN VFG0812 Protein 4e-94 51
yscN YP_004677249.1 ATP synthase yscN VFG0730 Protein 4e-94 51
yscN YP_004677249.1 ATP synthase yscN VFG2459 Protein 7e-88 47
yscN YP_004677249.1 ATP synthase yscN VFG0555 Protein 6e-83 45
yscN YP_004677249.1 ATP synthase yscN VFG1254 Protein 2e-83 44
yscN YP_004677249.1 ATP synthase yscN VFG2499 Protein 3e-75 44
yscN YP_004677249.1 ATP synthase yscN VFG2331 Protein 2e-74 43
yscN YP_004677249.1 ATP synthase yscN VFG1008 Protein 2e-81 43
yscN YP_004677249.1 ATP synthase yscN VFG1778 Protein 2e-79 43