Gene Information

Name : Runsl_4460 (Runsl_4460)
Accession : YP_004657917.1
Strain : Runella slithyformis DSM 19594
Genome accession: NC_015703
Putative virulence/resistance : Virulence
Product : Restriction endonuclease, type I, EcoRI, R subunit/type III
Function : -
COG functional category : V : Defense mechanisms
COG ID : COG0610
EC number : -
Position : 5151124 - 5154162 bp
Length : 3039 bp
Strand : -
Note : PFAM: Type I restriction enzyme R protein N terminus (HSDR_N); Type III restriction enzyme, res subunit; TIGRFAM: type I site-specific deoxyribonuclease, HsdR family; COGs: COG1373 ATPase (AAA+ superfamily); InterPro IPR003593; KEGG: sli:Slin_6960 transcr

DNA sequence :
ATGCCCAGCCAAACCAACGAACAAGCCTTAGAATCGGCCATTGAAAAACGCCTCACGGGCACCTGTCTGGAAGACCTCAA
TACCAACGGTGCAGTTGGCAACGTAGCCGAGCGGGCCGAGCTGTATCGTGCCGGCAACGGGTACTATCTTGGTACCCCGC
AGGACTTCAACGCCCAATACGCCGTGGATGAGCGTCGGCTGTGGCATTTTCTGGAAACCACCCAAAAAGAAGAACTCGCC
AAACTCCAAAAAAACGCCGACTGGAAACTCAAGATACTGGAGCGCATTGACCGCATGGTGAAGAAATACGGCATTTTGCG
CCTTCTACGTAAAGGGCTGGAAGTGGAAGATGCGCATTTTACCATGCTCTATTCGCTGCCGTTGGCTTCCAGCAGTCAAG
CCGTCAAAGATAATTTTGAAAGCAATGAGTTCAGCGTCACGCGCCAAATACGGTATTCGCTCACCAATCCCCGCGAAGAG
ATCGACATGGTGCTGTTTGTGAATGGGTTACCGCTCGTCACGATGGAGCTCAAAAACCAATGGACGGGCCAAAATGCCAA
AGTACACGGCCAAAACCAATACCGAAACGACCGCGACACCACCCAACCGCTGTTGCATTTCGGACGCTGCATTGTCCATT
TCGCCGTTGACCCCGACGAAGCCTACATGACCACCAAACTCAATGGCAAAGACACGTTCTTTTTACCCTTCAACTTGGGC
CACAACTACGGCAAAGGCAACCCGCCCAATCCTTTTGGTCACAAAACCGCCTATCTGTGGGAGGTCTTCACGCGCCAAAG
CTTAACCAATATCATTCAGCATTTTGTCCGTTTTGACGGCAAAGACACCGATACGTTGGCCAAGAGAAACCTGTTTTTTC
CGCGTTATCATCAACTCGACGTGGTGCGCAAGCTCATTCAGGATGCGAGCAAAAAAGGCGTCGGCCAAACCTATTTGATT
CAGCATTCGGCCGGTTCGGGCAAATCCAACTCCATCACGTGGGCGGCGTATCAGCTCATCGAAACTTACCCCGAAAACGA
CACCGTTCCCGGCAGTCGGGGAATCACCCACCCGCTCTTTGATTCCGTCATCGTCGTCACCGACCGCCGACTGCTCGACA
AGCAGTTGCGCGAGAACATCAGCGATTTTTCGGAAGTAAAAAATATAGTAGCCCCCGCCCATTCGTCCAAAGAGCTCAAA
GAGAGTTTGGAAAGCGGTAAGCGCATCATCATTACCACCATTCAGAAGTTCCCGTTTATTGTGGACGGCATTGCCGACAT
GAGCCACAAACGCTTTGCGGTCATCATCGACGAAGCCCACAGCAGCCAAAGCGGCTCCACCGCCGACAACATGAACCGTG
TCATGGGCAAGAAAGCTGAAGAGGAGGAAGAAGAAGACCCGGAAGATATTATTTTGAAAGCGATGAAAACCCGCAAAATG
CGCGGCAATGCGTCGTATTTAGCCTTTACGGCCACGCCCAAGAATGCCACACTGGAGAAATTCGGCATCAGGCAGGAAGA
CGGGAAGTTTAAGCCTTTTCACGAATACACCATGAAGCAGGCCATTGAAGAGGGCTTTATTCTGGACGTGTTGGCCAACT
ACACCACCTACAAAAGCTATTACGAAATTGAAAAATCCATTGAAGACAATCCGCTCTTTGATACGGCCAAAGCCCAAAAG
AAACTGAAAGCTTACGTAGAGCGGGACGCCCGGACCATTGCTACCAAAGCCGACATCCTACTCGATCACTTCATTACCAA
AGTGGTCAACCCCAAAAAGCTGAAGGGCAAAGCCAAGGCAATGGTGGTCACGCAGAGCATCGAATCGGCTATTCGGTATT
TCTTTGCGTTACGGCAATTGCTCAACGAGAAAGGCAACCCCTTCAACATTGTTATTGCATTTTCGGGCAAGAAAACCGTG
GACGGCATCGAATACACCGAAGAAAGCATCAATGGTTTTGCCGAAAAAGACACCCGTGACAAATTTGACCAAGACGACTA
CCGGATGTTGGTGGTAGCCAACAAATACCTGACGGGTTTTGACCAACCCAAACTCACCGCCATGTACGTGGACAAGAAGT
TACAGGACGTTTTAGCCGTTCAGGCACTTTCCCGTTTAAACCGTTCGGCCGATAAATTGGGTAAGAAAACGGAAGATTTG
TTTGTGCTGGATTTCTTCAATTCAACCGACGATATTAAGGTCGCATTCGACCCGTTCTACACGGCCACCACGCTCAGCAA
AGCCACCGATGTCAACGTATTGCACGATTTAAAAGGCCATCTGGACGATGTTGGCGTATATGAATGGGCAGAGGTAGAAG
ATTTTGTGGCCAAGTATTTTAAAGGCGTCAACGCCGAGCAACTAAGCCCCATCATTGACCTCGCCGCCGACCGTTTTAAC
CAACAACTGGAATTGGAAGATGCCCAAAAAGCGGATTATAAAATCAAGGCCAAGCAGTTTGTGAAGATTTACGCCCAAAT
GGCTTCCATTCTGCCGTATGAAATCATCAAATGGGAAAAGCTGTTTTGGTTTTTGAAATTCCTCATTCCCAAACTCATCA
TCGTTGATGCCGGTTCGGAAGCCTTGGATGAATTGCTAAATTCCGTTGACCTTTCCACCTACGGATTGGAGCGGGTAAAA
CTCAATACCGCCATTGGTCTTGATGCTTCAGAAACAGAATTGGAGCCGCAAAACTCCAACGTGAGAGGTGTTCACGGAGA
CACTACTCAGGAAGACCCGCTGGATCTAATCATCAGGAGTTTTAACGAACGATGGTTTCAGGGTTGGGATTTAACGCCCG
AAGAACAACGGGTAAAGTTTGTCACCCTGGCGCAAAGTATTCAGGCGCACCCTGATTTCAAAAACAAGTATTCCGAGAAT
CAGGACTCCCAAAACCGAGAAATTGCGTTCCGGAAAATTTTTGACGAAGTAATGGCCAAGCAACGCAAATCCGAGCTCGA
CCTGTATCGGCTTATCTCCAAAGACGATGCCTTCAAAACTGCCATGCAGGATACGTTAAAAAGGATGATACGCCCCTAA

Protein sequence :
MPSQTNEQALESAIEKRLTGTCLEDLNTNGAVGNVAERAELYRAGNGYYLGTPQDFNAQYAVDERRLWHFLETTQKEELA
KLQKNADWKLKILERIDRMVKKYGILRLLRKGLEVEDAHFTMLYSLPLASSSQAVKDNFESNEFSVTRQIRYSLTNPREE
IDMVLFVNGLPLVTMELKNQWTGQNAKVHGQNQYRNDRDTTQPLLHFGRCIVHFAVDPDEAYMTTKLNGKDTFFLPFNLG
HNYGKGNPPNPFGHKTAYLWEVFTRQSLTNIIQHFVRFDGKDTDTLAKRNLFFPRYHQLDVVRKLIQDASKKGVGQTYLI
QHSAGSGKSNSITWAAYQLIETYPENDTVPGSRGITHPLFDSVIVVTDRRLLDKQLRENISDFSEVKNIVAPAHSSKELK
ESLESGKRIIITTIQKFPFIVDGIADMSHKRFAVIIDEAHSSQSGSTADNMNRVMGKKAEEEEEEDPEDIILKAMKTRKM
RGNASYLAFTATPKNATLEKFGIRQEDGKFKPFHEYTMKQAIEEGFILDVLANYTTYKSYYEIEKSIEDNPLFDTAKAQK
KLKAYVERDARTIATKADILLDHFITKVVNPKKLKGKAKAMVVTQSIESAIRYFFALRQLLNEKGNPFNIVIAFSGKKTV
DGIEYTEESINGFAEKDTRDKFDQDDYRMLVVANKYLTGFDQPKLTAMYVDKKLQDVLAVQALSRLNRSADKLGKKTEDL
FVLDFFNSTDDIKVAFDPFYTATTLSKATDVNVLHDLKGHLDDVGVYEWAEVEDFVAKYFKGVNAEQLSPIIDLAADRFN
QQLELEDAQKADYKIKAKQFVKIYAQMASILPYEIIKWEKLFWFLKFLIPKLIIVDAGSEALDELLNSVDLSTYGLERVK
LNTAIGLDASETELEPQNSNVRGVHGDTTQEDPLDLIIRSFNERWFQGWDLTPEEQRVKFVTLAQSIQAHPDFKNKYSEN
QDSQNREIAFRKIFDEVMAKQRKSELDLYRLISKDDAFKTAMQDTLKRMIRP

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
VC1765 NP_231400.1 type I restriction enzyme HsdR Not tested VPI-2 Protein 0.0 67
VC0395_A1363 YP_001217306.1 type I restriction enzyme HsdR Not tested VPI-2 Protein 0.0 67
VPI2_0013c ACA01830.1 type I restriction enzyme HsdR Not tested VPI-2 Protein 0.0 67

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Runsl_4460 YP_004657917.1 Restriction endonuclease, type I, EcoRI, R subunit/type III VFG1098 Protein 0.0 67