Gene Information

Name : Geoth_0898 (Geoth_0898)
Accession : YP_004586977.1
Strain : Geobacillus thermoglucosidasius C56-YS93
Genome accession: NC_015660
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.9.2
Position : 872490 - 874253 bp
Length : 1764 bp
Strand : +
Note : TIGRFAM: Pyruvate kinase; KEGG: gmc:GY4MC1_0829 pyruvate kinase; PFAM: Pyruvate kinase, barrel; Pyruvate kinase, alpha/beta; PEP-utilising enzyme, mobile region

DNA sequence :
ATGATGCGGAAAACGAAAATCGTTTGCACGATTGGTCCTGCTAGCGAAAGCGTGGACAGGCTTGTGGAATTGATCAATGC
TGGAATGAACGTGGCGCGTTTGAATTTTTCCCACGGCGATTATGCAGAACATGGACGCCGCATTCAAAACATTCGCGAAG
CGGTAAAACGGACAGGAAAAACAGTAGCGATCCTTTTAGACACAAAAGGCCCGGAAATTCGCACACATAATATGGAAAAC
GGAGCGATTGAGCTAAAAGAAGGAGAACAGCTTGTCATTTCGATGCAAGAAGTGCTTGGGACACCGGAGAAAATTTCCGT
TACGTATGAAAAGCTAGTTGATGATGTCGCTCCTGGGGCAAAAATTCTTCTCGATGACGGTTTAATCGGGTTAGAAGTGA
TTTCGGTTGATCGGCAAGCGCGTGAAATCGTGACAAAAGTATTAAATGGCGGCGTGCTGAAAAACAAAAAAGGAGTTAAC
GTGCCTAGGGTTCGCGTCAATTTGCCGGGCATTACAGAAAAAGACCGCCAAGACATTTTGTTTGGCATCGAGCAAGGCAT
TGATTTTATCGCTGCTTCATTTGTGCGCCGCGCTTCTGACATATTAGAAATCCGTGAACTGCTTGAGGCAAACGACGCGC
TTCATATTCAAATCATTGCTAAAATCGAAAATCAAGAAGGCGTTGATAATATTGATGAAATTTTAGAAGTCGCGGATGGG
CTTATGGTGGCGCGCGGCGACCTTGGCGTGGAAATTCCAGCTGAAGAAGTGCCGCTTATCCAAAAAGTGCTGATCAAAAA
ATGCAATATGCTTGGTAAACCGGTTATTACGGCTACACAAATGCTTGATTCAATGCAGCGTAACCCAAGACCGACGCGGG
CAGAGGCAAGCGATGTCGCGAATGCGATTTTCGATGGAACAGATGCAGTGATGTTATCTGGAGAAACAGCGGCCGGACAC
TATCCGGTAGAAGCGGTTAAAACCATGCACCAAATTGCTTTGCGCACAGAACAAGCGCTGCAATACCGGGAACTATTAGC
ACAACGGACAAAAGAGAGCGCGACGACGATTACCGATGCAATTGGCCAGTCTGTTGCGCATACGGCGCTGAATCTTGACG
TGGCTGCAATTGTAACGCCGACGGTGAGCGGGAGAACAGCAGGCATGGTGTCAAAATACCGCCCGAAAGCACCGATTGTA
GCGGTTACTGCCGACGAATCGGTATCGCGTAAATTGGCGCTCATATGGGGAGTATATTCGCAAGTTGCCCCACAAGTGAA
CACGACCGATGAAATGCTTGATATTGCCGTAGAAGCGGCGATAAAATCAGGAGTAGTTAAACATGGTGATTTAGTCGTCA
TTACCGCGGGAGTTCCAGTTGGAGAAACGGGATCGACGAACTTGATGAAAGTGCATGTGATCGGGGACATTATCGCGAAA
GGCCAAGGAATCGGCCGTAAGTCTGCGTTCGGAAAGGTGATTATTGCGAACACGGCGGAAGAGGCCATCAACAAAATGGT
AGAAGGCGGAATTTTAGTAACGCACTGCACGGATGCCGACATGATGGAAGCGCTGAAGAAAGCGGCGGCGATCATTACCG
AGGAAGGCGGATTAACGAGCCACGCCGCAGTCGTCGGTCTAAACCTCGGCATTCCTGTTGTTGTCGGTGTAGAAAACGCG
ACATCGATTTTAAAAGACGGGCAGGAGATTACAGTCGATGCCGGATTTGGCGCCGTGTATCAAGGGCATGCAAGCGTGTT
ATAA

Protein sequence :
MMRKTKIVCTIGPASESVDRLVELINAGMNVARLNFSHGDYAEHGRRIQNIREAVKRTGKTVAILLDTKGPEIRTHNMEN
GAIELKEGEQLVISMQEVLGTPEKISVTYEKLVDDVAPGAKILLDDGLIGLEVISVDRQAREIVTKVLNGGVLKNKKGVN
VPRVRVNLPGITEKDRQDILFGIEQGIDFIAASFVRRASDILEIRELLEANDALHIQIIAKIENQEGVDNIDEILEVADG
LMVARGDLGVEIPAEEVPLIQKVLIKKCNMLGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAVMLSGETAAGH
YPVEAVKTMHQIALRTEQALQYRELLAQRTKESATTITDAIGQSVAHTALNLDVAAIVTPTVSGRTAGMVSKYRPKAPIV
AVTADESVSRKLALIWGVYSQVAPQVNTTDEMLDIAVEAAIKSGVVKHGDLVVITAGVPVGETGSTNLMKVHVIGDIIAK
GQGIGRKSAFGKVIIANTAEEAINKMVEGGILVTHCTDADMMEALKKAAAIITEEGGLTSHAAVVGLNLGIPVVVGVENA
TSILKDGQEITVDAGFGAVYQGHASVL

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 3e-113 53
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 1e-113 53
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 1e-113 53
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 2e-113 53

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Geoth_0898 YP_004586977.1 pyruvate kinase VFG0479 Protein 6e-114 53