Gene Information

Name : Geoth_0189 (Geoth_0189)
Accession : YP_004586326.1
Strain : Geobacillus thermoglucosidasius C56-YS93
Genome accession: NC_015660
Putative virulence/resistance : Virulence
Product : 2,3-dihydroxybenzoate-AMP ligase
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : 2.7.7.58
Position : 182227 - 183852 bp
Length : 1626 bp
Strand : +
Note : TIGRFAM: 2,3-dihydroxybenzoate-AMP ligase; KEGG: gmc:GY4MC1_0169 2,3-dihydroxybenzoate-AMP ligase; PFAM: AMP-dependent synthetase/ligase

DNA sequence : 
ATGCTAAGCGGATGCCCAACATGGCCAAAGGAGTTTGCAGACTTTTACCGGAAGGAGGGCTGCTGGGCAGGTGAGACTTT
TGGTGACATGCTGCGGAAAAGGGCGTTAAAGTACGGTGATCGCATTGCAGTTACTTCTGGAGAAAGGCAAATCAGCTATA
AAGAACTGGATAGAAGAGCGGATCAGCTTGCCTCAGGATTCCGACAACTGGGGATTCGGCCAGCGGACCGCGTTGTCGTT
CAATTGCCGAATCGAATTGAATTTTTTGAAGTATGCTTTGCTTTATTCCGTCTGGGGGCGCTGCCTGTCTTTGCCTTGCC
TTCACATCGATATAGTGAAATCAGTTATTTTTGTGAATTTAGCGAAGCGGCGGCTTATATTATTCCAGACCAGCATGGGG
GATTTGATTTCCGCTCCCTTGCCAAGCAGGTGAAGGCTGCTGTTCCAACCCTAAAACACGTCATTGTGGTTGGAGAGGCA
GGAGAGTTTGAATCATTAGAAAATCTGTTTATCGATCCTGTCCAGCTGCCGCTTGTTCATTCAAGCGATGTTGCTTTCTT
CCTGCTGTCCGGCGGAAGCACAGGGCTGCCAAAAATGATTCCGCGGACCCACGATGATTATATTTACAGTCTCCGGGTTA
GTGCCGAGATTTGTAAGCTTCATCAAGATAGTGTTTATCTTGCGGTTCTTCCCATTGCTCATAATTATCCGCTTAGTTCA
CCGGGTGTGCTAGGAACGTTGTATGCCGGCGGAAAAGTGGTCCTTGCAGACAGCCCAAGTCCTGATGAGGCATTTCCGCT
TATTCAGCAAGAGAAAGTCACCATTACTGCGCTCGTGCCGCCGCTTGTTTGGATATGGTTAGATGCCGCATCTTCTTGCA
AGTATGATTTATCCAGCCTTCAAGTTTTGCAAGTCGGCGGGGCGAAATTCAGCGCAGAAGTAGCCAAAAGAGTAAAACCA
GTTCTTGGCTGCACCTTGCAGCAGGTGTATGGCATGGCTGAAGGGCTGGTCAACTATACGAGATTGGATGATCCTGAAGA
ATTGATTATAAATACTCAAGGAAAGCCAATGTCTTACTATGATGAAATCCGCATAGTAGATGAAGAAGATAACGAGGTGG
AGCCTGGCCAAGTTGGAGAGCTGTTAACGAGGGGCCCTTATACCATTCGCGGATATTATAAAGCGGAAGCGCATAATGCG
AAAGCCTTTACTGCGGACGGCTTTTACCGGACGGGCGACTTGGTGCGGATGACTCCATTCGGCTACTTGGTTGTCGAAGG
GCGCGTGAAAGATCAAATTAACCGGGGCGGTGAAAAAATTGCGGCGGAGGAAGTGGAAAATCACCTTTTAGCACATCCTT
CCGTACGTGATGCGGCTGTCGTTGCGATGCCGGATGAATTCCTTGGCGAACGATCGTGCGCTTTTATCATTCTGCGTGAT
CACTCTTTGCAAGCAGCTGAACTGAAGGCGTTTTTGCAAGAGCGCGGAGTGGCAAGTTTTAAAATACCTGATCGCATCGA
ATTCGTTGATTCATTTCCGAAGACAGCGGTTGGAAAAATTAACAAGAGGGCATTACGGCAAATCATTGCCGAAAAACGGC
AATCTGCAGGAAAAACAAACGTATAA

Protein sequence : 
MLSGCPTWPKEFADFYRKEGCWAGETFGDMLRKRALKYGDRIAVTSGERQISYKELDRRADQLASGFRQLGIRPADRVVV
QLPNRIEFFEVCFALFRLGALPVFALPSHRYSEISYFCEFSEAAAYIIPDQHGGFDFRSLAKQVKAAVPTLKHVIVVGEA
GEFESLENLFIDPVQLPLVHSSDVAFFLLSGGSTGLPKMIPRTHDDYIYSLRVSAEICKLHQDSVYLAVLPIAHNYPLSS
PGVLGTLYAGGKVVLADSPSPDEAFPLIQQEKVTITALVPPLVWIWLDAASSCKYDLSSLQVLQVGGAKFSAEVAKRVKP
VLGCTLQQVYGMAEGLVNYTRLDDPEELIINTQGKPMSYYDEIRIVDEEDNEVEPGQVGELLTRGPYTIRGYYKAEAHNA
KAFTADGFYRTGDLVRMTPFGYLVVEGRVKDQINRGGEKIAAEEVENHLLAHPSVRDAAVVAMPDEFLGERSCAFIILRD
HSLQAAELKAFLQERGVASFKIPDRIEFVDSFPKTAVGKINKRALRQIIAEKRQSAGKTNV

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 6e-122 51
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 1e-121 51
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 9e-122 51
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 9e-122 51
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 9e-122 51
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 9e-122 51
ybtE CAA21394.1 - Virulence HPI Protein 9e-122 51
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 8e-122 51
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 7e-122 51

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Geoth_0189 YP_004586326.1 2,3-dihydroxybenzoate-AMP ligase VFG0168 Protein 2e-138 54
Geoth_0189 YP_004586326.1 2,3-dihydroxybenzoate-AMP ligase VFG0932 Protein 2e-118 50
Geoth_0189 YP_004586326.1 2,3-dihydroxybenzoate-AMP ligase VFG1817 Protein 2e-114 47