Gene Information

Name : Isova_1466 (Isova_1466)
Accession : YP_004542121.1
Strain : Isoptericola variabilis 225
Genome accession: NC_015588
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 1595458 - 1596894 bp
Length : 1437 bp
Strand : +
Note : TIGRFAM: Pyruvate kinase; KEGG: ske:Sked_20690 pyruvate kinase; PFAM: Pyruvate kinase, barrel; Pyruvate kinase, alpha/beta

DNA sequence :
ATGCGCAGAGCGAAGATCGTTTGCACCATCGGACCCGCGACGGCGTCCTCGGAGAAGCTCCGGGAGCTGGTCGACGCGGG
CATGGACGTGGCCCGCATCAACCGGAGCCACGGTGAGCAGGCGGAGCACGAGGCCGTCTACCACGGGGTGCGGAAGGCGG
CGGATGACGCCGGGCGCAACGTCGCCGTCCTCGTCGACCTCCAGGGCCCCAAGATCCGCCTGGGCCGCTTCGCGAACGAC
GAGAAGCACGTCCTGAACGAGGGCGACACGTTCACGATCACGACCGAGGACGTCGTCGGCACCCGCGAGCTCGTCTCGAC
GACGCACAAGGGCCTGCCCGACGACGCGCGCGTCGGCGACCCGATCCTCATCGACGACGGCAAGGTCCGCGTGCGCGTCA
CGGCCGTCGACGGCCCGCGCGTCGTGACGACCGTCGAGGTGCCCGGCCCGGTCTCGAACAACAAGGGCCTCAACCTGCCC
GGCGTCGCCGTGTCGGTGCCCGCGCTGAGCGACAAGGACACCGACGACCTGCGGTGGGCGCTGCGCCTGGGGGCCGACCT
CATCGCGCTGTCGTTCGTGCGCTCCGCGAAGGACTACGAGGACGTCGCGAAGATCATGGCGGAGGAGGGCCGCACGGTCC
CGGTCATCGCCAAGATCGAGAAGCCGCAGGCCGTCGAGAACCTCGAGGAGGTCGTGGCGGCGTTCGACGGCTTCATGGTC
GCGCGCGGCGACCTCGCCGTCGAGATGCCGCTCGAGCAGGTCCCGCTCGTGCAGAAGCGCATCGTCGAGCTGGCGCGCCG
CAACGCCAAGCCCGTCATCGTGGCGACCCAGGTCCTGGAGTCGATGACGACCAACCCGCGCCCCACCCGTGCCGAGGCGT
CGGACTGCGCCAACGCGGTGCTCGACGGCGCGGACGCGGTCATGCTCTCGGGCGAGACGAGCGTCGGCAACTACCCGATC
CTCACGGTCCAGACCATGGCCCGCATCATCGAGGCGACCGAGGAGATGGGCCGCGAGCGCATCGCCCCGCTCGGCTCCAC
GCCGCACACGCGCGGCGGCATCATCACCCGCGCAGCCGCCGAGATCGGCGACTCGCTCGGCGTGAAGTACCTCGTGACGT
TCACGCAGTCGGGCGACTCGGCCAAGCGCATGTCGCGCCTGCGCTCGGGCATCCCGCTGCTCGCGTTCACGCCCGAGGAG
AGCGTGCGCCACGTGCTCGCGCTGACCTGGGGCACCACGACGTACCAGGTGCCCAAGGTCGACAGCGTGGACGCCATGGT
CGGCCAGGTCGACTCGACGCTGCAGGCCAACGGCCTGGCCGAGCCCGGCGACCACGTCGTGATCGTCTCGGGCGCGCCCG
TGGGCGTGCCGGGCACGACGAACTCGATCCTCGTGCACCAGGTCGGCAGCTCGGACCTCCACACGATCACGCATTGA

Protein sequence :
MRRAKIVCTIGPATASSEKLRELVDAGMDVARINRSHGEQAEHEAVYHGVRKAADDAGRNVAVLVDLQGPKIRLGRFAND
EKHVLNEGDTFTITTEDVVGTRELVSTTHKGLPDDARVGDPILIDDGKVRVRVTAVDGPRVVTTVEVPGPVSNNKGLNLP
GVAVSVPALSDKDTDDLRWALRLGADLIALSFVRSAKDYEDVAKIMAEEGRTVPVIAKIEKPQAVENLEEVVAAFDGFMV
ARGDLAVEMPLEQVPLVQKRIVELARRNAKPVIVATQVLESMTTNPRPTRAEASDCANAVLDGADAVMLSGETSVGNYPI
LTVQTMARIIEATEEMGRERIAPLGSTPHTRGGIITRAAAEIGDSLGVKYLVTFTQSGDSAKRMSRLRSGIPLLAFTPEE
SVRHVLALTWGTTTYQVPKVDSVDAMVGQVDSTLQANGLAEPGDHVVIVSGAPVGVPGTTNSILVHQVGSSDLHTITH

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 1e-77 43
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 1e-77 43
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 3e-77 43
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 3e-77 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Isova_1466 YP_004542121.1 pyruvate kinase VFG0479 Protein 6e-78 43