Gene Information

Name : Metme_4222 (Metme_4222)
Accession : YP_004515072.1
Strain : Methylomonas methanica MC09
Genome accession: NC_015572
Putative virulence/resistance : Unknown
Product : Restriction endonuclease, type I, EcoRI, R subunit/Type III
Function : -
COG functional category : V : Defense mechanisms
COG ID : COG0610
EC number : -
Position : 4643193 - 4646279 bp
Length : 3087 bp
Strand : +
Note : KEGG: dze:Dd1591_4250 protein of unknown function DUF450; PFAM: Restriction endonuclease, type I, EcoRI, R subunit/Type III, Res subunit, N-terminal; Restriction endonuclease, type I, R subunit/Type III, Res subunit; SMART: DEAD-like helicase, N-terminal

DNA sequence :
ATGGCGCAAACCACCGAAAAAGCCTTCGAAAGCTATGTCGAGCAAATGCTGCTGGCCAAAGGCTGGCAAGCGAGTTCTGT
AAAGGAGTGGGATAAGGAGCTGGCTTTATTCCCTACGCAAATTCTCGATTTTATCGCCGCCACCCAAACGCCGCTTTGGG
ACAGTATGCGCTCGCAACATGGCGCTAATCTGCCAGCCATGTTGATCAAAGAGCTGGTTAAGGAATTGGCCATCAAAGGC
GCGTTGCATGTGTTGCGCCACGGCTTTAAGTTTTACGGCAAGACCTTTCGGCTAGCTTATTTCAAACCGGCGCACGGCTT
GAATGCCGAGGTGTTGGCGCATTATCAACACAACCGGCTGACGGTAACGCGGCAGGTGCCCTGCCATCCGGGCGAGCATA
GTACGCTGGACATGCTGTTTGCGGTTAATGGTTTGCCGGTGGCAAGCTGCGAACTGAAAAACCCTTGGACCGGGCAAAAC
TGGCGGCATGCGATAAATCAATATCGCAACGACCGCAATCCGCGCGCGCCATTGTTCGAATTTAAACAACGGGCGCTGGT
GCATTTTGCCGCCGACCCGGACGAAGTCCATATGACCACCCGGCTGGCCGGTAAAAAAACCTTTTTCCTGCCGTTTAATC
GCGGCAGTCATCCCGGCGAAGTAGTCTGCGGGGCGGGCAATCCGCAACATCCATCCGGCTACCGCAGCGGATATTTCTGG
GAAGAGGTGTTGGAGCGCGAACAGTTTCTGGACATCCTGGGTAATTTTATTTTCATTGAGCGCAAGGAAGAGAAAGTCGA
AGACGGCAAGGGCGGCAGTCGCTTGTTGCAGAAAGAAGCGGTGATTTTTCCGCGTTATCACCAACTGGATGCCACCCGCC
GATTGGTCAATGCTGCGCAACTGGAAGGCCCCGGCCAGAATTATCTGATTCAGCATTCGGCGGGCAGCGGCAAAACCAAT
TCGATTTCCTGGCTGTCGCATCGCTTAGCCAGTTTGCACAATGCCGATGAGCAAAAGGTATTCGATTGCGTGATTGTGAT
TACCGATCGGCAAGTGCTGGACAGGCAATTGCAGGACGCGATTTATCAAATCGAACACGCGCAAGGCGTGGTGAAGGCTA
TCGATCAGGATTCCAAACAGTTGGCGGCGGCGCTGATCGACGGCACCAAGATCGTGATTACCACCTTGCAGAAGTTTCCG
TTTGTGTTGCGCGGCTTATTGCATGCTGCTGGCGCCGATAATATCGACGCAGCGGACGAGATGGCTAAAACGCAAGCCAA
AGCTTGGGAAATGGAGATCGGCAAGCGGTGTTATGCGGTGATTGTCGATGAAGCGCATTCGTCGCAAACCGGGGAAACAG
CCCGAGAACTCAAGTCGATTTTGGGTGCGAATGCCGCCAACGGTGAAGATGAAGAAACCGATTTTGAAGACCGCTTGAAT
CAGGTAATGGCTTCGCGCGGTCGTCAGCCCAACATCAGCTTTTTTGCCTACACCGCCACACCCAAGGGTAAGACCTTGGA
GCTGTTCGGTCGAGTCGGTCACACAGGCAAGCCTGAGCCGTTTCACCTGTACAGCATGCGCCAAGCCATTGAAGAAGGTT
TTATTCTGGACGTGTTGCAGAATTACACGACCTACAAAACCTATTTCAAATTGGTTAAAGCCATGGAGGATGACCCGAGT
CTGCCCAAGAAAAAGGCGGCGCGGGCCTTGGCCAAATTCCTGGTATTGCATCCGACCAATATTTCCCAAAAAATCGAAAT
CATCGTCGAACATTTCCGCAACCACGTTAAACATCATTTAGGCGGCCAGGCCAAGGCCATGGTGGTGACCAGTACCCGCT
TGCAGGCGGTTAAGTACGTGCAGGCATTTCAGGCTTATATCGAGCAGCACGGTTACCGCGATGTACAGCCCTTGGTAGCG
TTTAGCGGCACGGTACTCGATCCCGACAGTGGGCAGGAATACACCGAACCGGGCATGAATATCGACAGCGTGACCGGTAA
GGCTATTAGCGAAAAACAGTTGCCGGAGCGCTTTAATTCACCCGATTATCAAGTGCTGCTGGTCGCCAACAAGTACCAAA
CCGGTTTCGATCAGCCTAAGTTGATGGCAATGTATGTCGATAAGCGTTTGGATGGTGTGCAGGCGGTGCAAACCCTATCG
CGCCTGAATCGCATGCTGCCGGGTAAGGAAGCGCCGTTTGTGCTGGATTTTGTCAACGAAGCCGAAGACATTTACCGCGC
GTTCAAACCTTATTACGACGCCACCAGCTTACAGGAAACGTCCGATCCGGCTTTACTGGAACAGGTCAAGCATGACTTGG
ACAGTTTGCAGGTTTATTACTGGAACGAAGTGGAAGCGTTTGCGCGTATTTTCTACCGTTCACCGAATAAACAAAATCCA
GCCGATCACGCCCATTTGCAACGCCATTTACAACCGGCGGTGGACCGGTTCAAGGCGATAAGCGATGACGAGCAGCGCTC
TGTATTTCGGGACAAGTTAAGCGGCTATGTCAATGTCTATGCTTTTTTGAGTCAGATCATGCCTTATGCCGATCCTGATT
TGGAAATGCTGTATAGCTTTGGCCGTTTGTTATTGCCGCACTTGCCGCTGGATAGAGATAACAGCAACGTCAAGCTGGGC
GACGAAGTCGATTTGCACTATTACCGGCTGCAGCGGGTGTTTTCCGGCGCGATCGATCTGGCGGATGAACAAGGCGAGTA
CACGGTTAAAAGTCCGACCGATGTCGGCACCGGTAAAGCCAAGGACGAAAAAGCCCCACTGTCTGAAATCATCGAGCTAT
TAAACAACCGGTTTGGCACGAACTTTACGGAAGAAGACCGCTTGTTCTTCGAGCAGATCAAAGAAAAAGCCACCAATTCG
CCGGAGGTAATTCAGCTACGGCAAGCCAATCCGTTTGATAAGTTTCAGCTGGGGGTGCGGCAAATGTTGGAAGACTTGAT
GATTCAACGCATGAACGAGAACGACAAGATCGTCACTCGATACATGGATGATAGAGCGTTTGAGGATGCGGCGTTTGCGG
TGCTGTCTAAGGTCATTTTCGACAGTATTCCGCAAGCCAAGGATTAG

Protein sequence :
MAQTTEKAFESYVEQMLLAKGWQASSVKEWDKELALFPTQILDFIAATQTPLWDSMRSQHGANLPAMLIKELVKELAIKG
ALHVLRHGFKFYGKTFRLAYFKPAHGLNAEVLAHYQHNRLTVTRQVPCHPGEHSTLDMLFAVNGLPVASCELKNPWTGQN
WRHAINQYRNDRNPRAPLFEFKQRALVHFAADPDEVHMTTRLAGKKTFFLPFNRGSHPGEVVCGAGNPQHPSGYRSGYFW
EEVLEREQFLDILGNFIFIERKEEKVEDGKGGSRLLQKEAVIFPRYHQLDATRRLVNAAQLEGPGQNYLIQHSAGSGKTN
SISWLSHRLASLHNADEQKVFDCVIVITDRQVLDRQLQDAIYQIEHAQGVVKAIDQDSKQLAAALIDGTKIVITTLQKFP
FVLRGLLHAAGADNIDAADEMAKTQAKAWEMEIGKRCYAVIVDEAHSSQTGETARELKSILGANAANGEDEETDFEDRLN
QVMASRGRQPNISFFAYTATPKGKTLELFGRVGHTGKPEPFHLYSMRQAIEEGFILDVLQNYTTYKTYFKLVKAMEDDPS
LPKKKAARALAKFLVLHPTNISQKIEIIVEHFRNHVKHHLGGQAKAMVVTSTRLQAVKYVQAFQAYIEQHGYRDVQPLVA
FSGTVLDPDSGQEYTEPGMNIDSVTGKAISEKQLPERFNSPDYQVLLVANKYQTGFDQPKLMAMYVDKRLDGVQAVQTLS
RLNRMLPGKEAPFVLDFVNEAEDIYRAFKPYYDATSLQETSDPALLEQVKHDLDSLQVYYWNEVEAFARIFYRSPNKQNP
ADHAHLQRHLQPAVDRFKAISDDEQRSVFRDKLSGYVNVYAFLSQIMPYADPDLEMLYSFGRLLLPHLPLDRDNSNVKLG
DEVDLHYYRLQRVFSGAIDLADEQGEYTVKSPTDVGTGKAKDEKAPLSEIIELLNNRFGTNFTEEDRLFFEQIKEKATNS
PEVIQLRQANPFDKFQLGVRQMLEDLMIQRMNENDKIVTRYMDDRAFEDAAFAVLSKVIFDSIPQAKD

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
SAS0025 YP_042158.1 type I restriction enzyme protein Not tested SCC476 Protein 5e-175 41