Gene Information

Name : SerAS12_3614 (SerAS12_3614)
Accession : YP_004502031.1
Strain : Serratia sp. AS12
Genome accession: NC_015566
Putative virulence/resistance : Virulence
Product : 2,3-dihydroxybenzoate-AMP ligase
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : 2.7.7.58
Position : 3852291 - 3853919 bp
Length : 1629 bp
Strand : -
Note : TIGRFAM: 2,3-dihydroxybenzoate-AMP ligase; KEGG: spe:Spro_3420 enterobactin synthase subunit E; PFAM: AMP-dependent synthetase/ligase

DNA sequence : 
ATGAGCATTGATTTTACTCCCTGGCCTGAGGCGTTGGCCCGTCGTTATCGTGAACGCGGTTATTGGACCGACCGGCCATT
GACCGACATTATTGCCCGCCAGGCCAAAAATGACGCCGTGGCGCTGATCGACCCGCAGCGCAGCCTGAGCTATCGCCGGC
TCAATCAACTTTCTGACCGGCTGGCCGCCGCGCTGCAACGTCGGGGCTTGCACAGTGGCGATACCGCTCTGGTGCAGTTG
GGCAACGTGGTCGAGTTTTATGTGGCCTTCTTTGCGCTGTTGAAAATTGGCGTGGTGCCGGTGAACGCGCTGTTCAGCCA
TCAACGCAACGAACTCAAGGCTTATGCCGCACAAATCAAACCTGCGCTGCTGATTGCCGATCGCCAGCATGGCCTGTTCG
GCAACGACGAATTCCTCACGACGTTTCGCGACGAAAATCCTTCATTGCGCGTAGTGGGGCTGCGCAGCCAGGCGGAAGGC
GAGCAGTCGCTGGCCGCCTGGTTGGAAGAGGATACCGACGATTTTATCGCGACACCGACTCCGGCTGACCAGGTGGCGTT
CTTCCAGCTTTCCGGTGGCAGCACCGGCACGCCAAAGCTGATCCCGCGCACCCACAATGATTACTACTACAGCATCCGCC
GCAGCGTGGAAATTTGCCATTTCGATGCGCATACCCGCTACCTGTGCGCGCTGCCGGTGGCGCATAACTACCCGATGAGT
TCTCCGGGCGTACTCGGCGTGTTTTATGGCGCCGGTCTGGTGGTGTTCGCCAGCGATCCGGATGCCGGGCAATGCTTCCG
TTTGATCGAGCAGCACCAGATTAACGTGACGGCGCTGGTGCCGCCGGCGGTGACGCTGTGGCTACAGGCGATTGAAGAGT
GGGGCGGTGGTCAACCCTTGGCCAGCCTTAAGCTGTTGCAGGTGGGAGGCGCCAAGCTGAGTGAGACCTTGGCCGCGCGT
ATTCCGGCGGAAATCGGCTGCCAGTTGCAGCAGGTCTTCGGCATGGCGGAAGGTCTGGTGAATTACACCCGGTTGGACGA
TGACGATCGGCATATTCTCACCACTCAGGGCTGCCCGATGTCGCCGGACGACGAACTGTGGGTGGCCGATGACGAGGGTA
ACCGGCTGCCGGTGGGGCAAACCGGGCGCCTGATGACCCGGGGCCCTTACACCTTCCGCGGTTATTACCAAAGCCCCGAA
CATAACGCCGCCGCCTTTGATAAAGACGGTTTTTACTGTTCCGGGGATCTGATCAGCCTGACTGAAGACGGCTATGTGAA
GGTGGAAGGGCGTCAGAAAGATCAGATCAACCGCGGTGGGGAGAAGATCGCCGCCGAGGAGATCGAAAACCTGCTGTTGC
GCCACCCGGAGGTGATCAATGCGGCGCTGGTTTCCATGCCGGATGAGCTGATGGGCGAGAAGAGCTGCGCCTTTATTATC
GCCGCCAGCGCGTTGAAGCCGGTGGTGCTGCGCCGCCATTTGCGTGCAGAGGGCGTGGCCGAATTTAAATTACCTGACCG
CTTTATACAGGTCGATACGCTGCCGCTCACCCCGGTAGGAAAAGTGGATAAAAAACGGTTGCGTCAACGCCTGGAGACGC
AATCACTGACTCAGGCCCAGGGAGAATAA

Protein sequence : 
MSIDFTPWPEALARRYRERGYWTDRPLTDIIARQAKNDAVALIDPQRSLSYRRLNQLSDRLAAALQRRGLHSGDTALVQL
GNVVEFYVAFFALLKIGVVPVNALFSHQRNELKAYAAQIKPALLIADRQHGLFGNDEFLTTFRDENPSLRVVGLRSQAEG
EQSLAAWLEEDTDDFIATPTPADQVAFFQLSGGSTGTPKLIPRTHNDYYYSIRRSVEICHFDAHTRYLCALPVAHNYPMS
SPGVLGVFYGAGLVVFASDPDAGQCFRLIEQHQINVTALVPPAVTLWLQAIEEWGGGQPLASLKLLQVGGAKLSETLAAR
IPAEIGCQLQQVFGMAEGLVNYTRLDDDDRHILTTQGCPMSPDDELWVADDEGNRLPVGQTGRLMTRGPYTFRGYYQSPE
HNAAAFDKDGFYCSGDLISLTEDGYVKVEGRQKDQINRGGEKIAAEEIENLLLRHPEVINAALVSMPDELMGEKSCAFII
AASALKPVVLRRHLRAEGVAEFKLPDRFIQVDTLPLTPVGKVDKKRLRQRLETQSLTQAQGE

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 1e-87 43
ybtE CAA21394.1 - Virulence HPI Protein 1e-87 43
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 1e-87 43
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 2e-87 43
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 1e-87 43
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 1e-87 43
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 1e-87 43
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 2e-87 43
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-87 43

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
SerAS12_3614 YP_004502031.1 2,3-dihydroxybenzoate-AMP ligase VFG0932 Protein 0.0 70
SerAS12_3614 YP_004502031.1 2,3-dihydroxybenzoate-AMP ligase VFG0168 Protein 5e-109 47