Gene Information

Name : Lbuc_1030 (Lbuc_1030)
Accession : YP_004398352.1
Strain : Lactobacillus buchneri NRRL B-30929
Genome accession: NC_015428
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 1091529 - 1093286 bp
Length : 1758 bp
Strand : +
Note : TIGRFAM: Pyruvate kinase; KEGG: lbr:LVIS_0765 pyruvate kinase; PFAM: Pyruvate kinase, barrel; Pyruvate kinase, C-terminal-like; PEP-utilising enzyme, mobile domain

DNA sequence :
ATGAAGAAAACTAAGATCGTAAGTACATTAGGCCCAGCAAGTACTGATGTAGACACAATTACTAAGCTGATTGAAGCTGG
AGCTAATGTTTTCCGATTTAACTTTTCACATGGTGACCACCCTGAACATCTTGATCGTATGAACAAGGTTCACGAAGCTG
AAAAGAAGACTGGCAAGACTGTTGGAATTATGCTTGATACCAAAGGTGCTGAAATTCGGACAACTGTTCAAAAAGACGGC
AAGATCGAATTCCACACTGGCGATGTTTTCCGTATTTCAATGGACGCAAGTCTTGAGGGTACTAAAGACAAGATCGCGGT
TACTTACCCTGGACTCTTTGACGATGTTCACGAAGGCGGCCATGTTCTTTTCGATGATGGTTTGTTGGACACTGTTGTCA
CTAAAAAAGACGATGCTAACAAAGAATTAGTTGTCACTGTTCAAAACGACAGCACTCTTGGTTCACGTAAAGGTGTTAAC
GCACCCGGCGTTTCAATCAACCTGCCTGGTATCACTGAAAAAGATTCAGATGATATTCGTTTTGGTTTGGACAATGACAT
TGACTTCATTTCTGCTTCATTCGTTCGTAAAGCCCAAGATGTTTTGGACATCCGTGAATTGCTTGAAGAAAAGCATATGG
AACATGTTCAAATCTTCCCTAAGATTGAATCACAAGAAGGTATCAACAACTTTGACGATATCATCAAGGTATCTGATGGT
TTAATGGTTGCCCGTGGTGATATGGGTGTTGAAATTCCAGCAGAAAACGTTCCGTTGGTCCAAAAGACTTTGATCAAGAA
ATGTAACCAACTTGGCAAGCCGGTTATCACCGCTACTCAAATGCTTGATTCAATGCAGGAAAACCCACGTCCTACTCGTG
CCGAAGCTTCTGATGTTGCCAATGCCGTCTTTGATGGTACTGATGCAACGATGCTTTCTGGTGAAAGTGCTAATGGTGAT
TACCCTGTTGAATCAGTTCAAACGATGGCTCGTATCGATGTTAAGGCTGAAAATGCCTTTAGCGAATTTGGTACCCCACG
TCCAGAATTTGACTCAAGTGATGTTACTGAATCAATTGGTGACTCAGTTGCCCGTGTTGCCAAAGAATTAGGCGTTCACT
GCATCGTTGCAGCTACCCGTTCTGGTTACACTGCACGAATGATCTCCAAGTATCATCCAGATGCTGACATCCTGGCCATT
ACTTTCGACGAACGTACTCGTCGCGGTTTGATGGTTAACTGGGGTGTTCACCCAATCTTGGTTGACGAAGTTAAGAGCAC
TGATGAAATCTTTGAATTAGCTTCAAAGAAGGCTCTCGAAACTGGTTTAGCTAAGGAAGGCGATTTGATTATCGTTACTG
CCGGAGTTCCTGTTGGTGAGAGTGGTACCACCAACTTGATGAGAATCCAACTGATCGGTTCAAAGCTGGTTCAAGGTCAA
GGAGTTGGTGACAAGACTGTTATTGGTAAAGCGGTTGTTGCTAAAAACGCTGCTGATGCCAAGAGCAAAGTAACTGAAGG
CAGTATCTTGGTTACTAAGTCAACTGACAAAGAATACTTACCAGCTATTGAAAAGGCAAGCGCCGTAGTTGTTGAAACCG
GCGGCTTGACTTCACACGCAGCTGTTGTTGGTATTTCAATGGGAATCCCCGTTGTTGTTGGTGCAGCTGATGCTACCAGC
AAGATTTCTGACGGTGAATTGATTACCGTTGATTCACATCGGGGCGTTATTTACCATGGTGCTTCATCATCAATCTAA

Protein sequence :
MKKTKIVSTLGPASTDVDTITKLIEAGANVFRFNFSHGDHPEHLDRMNKVHEAEKKTGKTVGIMLDTKGAEIRTTVQKDG
KIEFHTGDVFRISMDASLEGTKDKIAVTYPGLFDDVHEGGHVLFDDGLLDTVVTKKDDANKELVVTVQNDSTLGSRKGVN
APGVSINLPGITEKDSDDIRFGLDNDIDFISASFVRKAQDVLDIRELLEEKHMEHVQIFPKIESQEGINNFDDIIKVSDG
LMVARGDMGVEIPAENVPLVQKTLIKKCNQLGKPVITATQMLDSMQENPRPTRAEASDVANAVFDGTDATMLSGESANGD
YPVESVQTMARIDVKAENAFSEFGTPRPEFDSSDVTESIGDSVARVAKELGVHCIVAATRSGYTARMISKYHPDADILAI
TFDERTRRGLMVNWGVHPILVDEVKSTDEIFELASKKALETGLAKEGDLIIVTAGVPVGESGTTNLMRIQLIGSKLVQGQ
GVGDKTVIGKAVVAKNAADAKSKVTEGSILVTKSTDKEYLPAIEKASAVVVETGGLTSHAAVVGISMGIPVVVGAADATS
KISDGELITVDSHRGVIYHGASSSI

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 1e-95 47
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 7e-96 47
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 7e-96 47
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 2e-95 47

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Lbuc_1030 YP_004398352.1 pyruvate kinase VFG0479 Protein 3e-96 47