Gene Information

Name : CbC4_0447 (CbC4_0447)
Accession : YP_004395124.1
Strain : Clostridium botulinum BKT015925
Genome accession: NC_015425
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 474037 - 475458 bp
Length : 1422 bp
Strand : +
Note : -

DNA sequence : 
ATGCAAAGAACTAAGATGGTTTTTACAATTGGACCTGCAAGTGAGAACAAAGAATATTTAAGAGAACTTATTTTAGCTGG
AATGAGCGCAGCTAGACTTAACTTTTCACATGGTGATTTTGAAGAACATGGTGGAAGAATCAAAACTATAAGAGAATTAA
GAAAAGAATTAAACAAACAAGTAGCAATAGTTTTAGACACTAAGGGACCTGAAATCAGAACTAAGGAATTCGAAGGAAAA
GTAAAATTAGTTAAAGGAAGCAAATTCACAATATATTGTACAGAAGATGTAATGGGAGATGCTACTAAATGTACTATTAC
ATATACTGACTTATATAAAGATGTTAAACCAGGAAATACTATATTAATAGATGACGGTTTAGTTGGTCTTACTGTAGAAT
CAATTGAAGATACTAAAATTCACTGCGTAGTTGCAAACAATGGTATTGTATCTAGCAAAAAAGGAGTTAACGTTCCAAAC
GTAAGCATAAAACTTCCTGCTATAACTGAAAAAGATAAGGGAGATTTAATCTTCGGATGTCAAGAAGAAGTTGATATGGT
TGCTGCTTCATTTATAAGAAAAGCAGATGACGTTTTAGCTATAAGAAAAGTTTTAGATGAAAATGGTGGAGAACACATAA
GAATATTCTCTAAAATAGAAAACCAAGAGGGTGTGGATAATGTTGATGAAATATTAGAAGTATCTGATGGTATCATGGTT
GCTAGAGGAGACATGGGAGTTGAAATTCCTATAGAACAAGTTCCAATAGTTCAAAAAATGATAATAGCTAAATGTAACAA
AGCTGGAAAACCAGTTATAACAGCAACACAAATGCTTGACTCAATGATTAGAAACCCAAGACCAACAAGAGCAGAAGCAT
CAGACATAGCAAACGCTATATTTGATGGAACAGATGCTACAATGTTAAGTGGAGAATCTGCTAATGGAGATTACCCAATT
CAAGCTGCTCAAACAATGGCTAGAATAGCTCAAGCTGCTGAAAAATACGTTGATCACAAAGCTAACTTAGAAAAAAGCAA
GCTAGAAAAAGTTGACAATATTGCAGATGCTATAAGCTTAAGTGCTTGCACAACAGCTATGGAATTAAATGCTGCTGCAA
TAATCGTACCAACTAAGACTGGTAACACAGCTAAAATGATCGCTAAATATAGACCAGAATGTCCAATCATAGCAGTAACA
CCAGATGACAAAATAACTAGAAGATTAGCTATATCATGTGGAGTATACGCATTAACTGCTACAGCTTTCAATTCAACTGA
TGAAATGATCGAAAAATCTGTTGCATTTGCTAAAGAAGCAGGACACGTTAAAGATGGAGATACTGTTGTAGTAGCTGCTG
GACTTCCAATACACGAATCAGGCACTACTAACATGATAAAAGTACACGTTGTAGGAAAATAA

Protein sequence : 
MQRTKMVFTIGPASENKEYLRELILAGMSAARLNFSHGDFEEHGGRIKTIRELRKELNKQVAIVLDTKGPEIRTKEFEGK
VKLVKGSKFTIYCTEDVMGDATKCTITYTDLYKDVKPGNTILIDDGLVGLTVESIEDTKIHCVVANNGIVSSKKGVNVPN
VSIKLPAITEKDKGDLIFGCQEEVDMVAASFIRKADDVLAIRKVLDENGGEHIRIFSKIENQEGVDNVDEILEVSDGIMV
ARGDMGVEIPIEQVPIVQKMIIAKCNKAGKPVITATQMLDSMIRNPRPTRAEASDIANAIFDGTDATMLSGESANGDYPI
QAAQTMARIAQAAEKYVDHKANLEKSKLEKVDNIADAISLSACTTAMELNAAAIIVPTKTGNTAKMIAKYRPECPIIAVT
PDDKITRRLAISCGVYALTATAFNSTDEMIEKSVAFAKEAGHVKDGDTVVVAAGLPIHESGTTNMIKVHVVGK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 2e-97 51
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 2e-97 51
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 2e-96 51
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 2e-97 51

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
CbC4_0447 YP_004395124.1 pyruvate kinase VFG0479 Protein 8e-98 51