Gene Information

Name : CbC4_1299 (CbC4_1299)
Accession : YP_004395976.1
Strain : Clostridium botulinum BKT015925
Genome accession: NC_015425
Putative virulence/resistance : Virulence
Product : tetanolysin O
Function : -
COG functional category : -
COG ID : -
EC number : -
Position : 1383796 - 1385442 bp
Length : 1647 bp
Strand : +
Note : putative conserved domain identified by match to Thiol_cytolysin superfamily

DNA sequence :
ATGAAAACTATCAAAAAAAAGTTAAGATTAGTAGCGCAAGTTATGGTAGTTATTATGATTATTGGATTAACATATACCGG
TAATTTTAATAATAATATTGTACTAGCTGATACAAATGATCAAAGTAGTAATACTAAAGATACTAGTAACAAGGAAAATG
AAAAATTAGAAACCGAGAAAAGTGGACAACCAGTTAAACCAAATGATCAAACTTCACAAATAAAAGATGAAAAAACTAAA
AAAAATAGTGAAGAAATAGATGAAAAAATTTATGGATTATCTTATGATCCAAAAAAGATTCTAACATCGAACGGAGAAAA
GATTGAAAATTTTGTACCGGCTGAAGGTTATGAAGATTCTGATAAATATGTTGTAATAAAACGTGAAAAGAAAAGTATTA
CAGATTCCACAGCAGACATCTCAATAATAGATTCAATAAACGATAGAACTTATCCTGGTGCTATACAACTTGCAAACAAG
AATCTTATAGAAAATAAGCCAGACTTGGTTTCATGTGAAAGAAAACCAATTACTATAAGCGTTGACTTACCTGGTATGAC
TAATGATGGTAAAAAGGTTGTTAAATCACCTACTTATTCTTCAGTTAATTCTGCAATAAATTCTATATTAGATACTTGGA
ATACAAAATATTCACAAAAATATACTATTCCTACAAGAGTTAGCTATTCTGATACCATGGTATATAGTAAGTCTCAATTA
TCAACAATGCTTGGATGCAATTTTAAAGCTATAGATAAGTCCTTAAACATAGATTTCAACTCTATATTTAAAGGTCAAAA
AAAAGTTATGGTTGTTGCTTACAAGCAAATATTTTATACAGTAAGTGTAGATGCGCCTAATCGCCCATCAGATGTGTTTG
GAGATAATGTTACATTTAATGAGCTAACTTTAAAAGGATTAAACAATACCAATCCTCCTGCATATGTTTCAAATGTTGCA
TATGGTAGAACAATATATGTAAAACTTGAGACAACATCTAAGAGTGGAAATGTTAAAGCAGCATTTAAAGCATTAATTGA
TAATCAAGACATAAGCAGTAATGCAGAATATAAAGATATATTAAATCAAAGTTCATTTACAGCTACTGTTTTGGGTGGAG
GAGCTCAAGAACATAATAAAATAGTTACTAAAGATTTTGATGAAATAAGAAATGTTATTAAAAATAATTCTGTATATAGT
CCACAAAATCCAGGATATCCAATTTCATATACTACTACATTTTTAAAAGACAATCATATAGCATCTGTAAACAATAAAAC
AGAGTATGTAGAAACAACAGCAACAGAATACAATAATGGTAAAATAGTACTTGACCATAGTGGAGCATATGTAGCTCAAT
TCCAACTAACATGGGATGAAGTTAGCTATGATAAACAAGGAAATGAAATAATTGAGCATAAAGGATGGTCAGGAAATAAT
ACTGATAAAACAGCTCATTTCAATACAGAGATCTATTTAAAAGGAAATGCAAGAAATATTTCTGTAAAAATAAAAGAGTG
CACAGGACTTGCATGGGAATGGTGGAGAACCATTGTAGATGTTAAAAATATACCTCTTGTAAAAGAAAGAACTTTCTATA
TATGGGGTACAACTCTATATCCTAAACATTCTATAGAGGAAAAATAA

Protein sequence :
MKTIKKKLRLVAQVMVVIMIIGLTYTGNFNNNIVLADTNDQSSNTKDTSNKENEKLETEKSGQPVKPNDQTSQIKDEKTK
KNSEEIDEKIYGLSYDPKKILTSNGEKIENFVPAEGYEDSDKYVVIKREKKSITDSTADISIIDSINDRTYPGAIQLANK
NLIENKPDLVSCERKPITISVDLPGMTNDGKKVVKSPTYSSVNSAINSILDTWNTKYSQKYTIPTRVSYSDTMVYSKSQL
STMLGCNFKAIDKSLNIDFNSIFKGQKKVMVVAYKQIFYTVSVDAPNRPSDVFGDNVTFNELTLKGLNNTNPPAYVSNVA
YGRTIYVKLETTSKSGNVKAAFKALIDNQDISSNAEYKDILNQSSFTATVLGGGAQEHNKIVTKDFDEIRNVIKNNSVYS
PQNPGYPISYTTTFLKDNHIASVNNKTEYVETTATEYNNGKIVLDHSGAYVAQFQLTWDEVSYDKQGNEIIEHKGWSGNN
TDKTAHFNTEIYLKGNARNISVKIKECTGLAWEWWRTIVDVKNIPLVKERTFYIWGTTLYPKHSIEEK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
slo YP_281505.1 streptolysin O Virulence Not named Protein 5e-126 56
hly AAA69525.1 listeriolysin O Virulence LIPI-1 Protein 1e-117 46

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
CbC4_1299 YP_004395976.1 tetanolysin O VFG2275 Protein 2e-135 60
CbC4_1299 YP_004395976.1 tetanolysin O VFG0976 Protein 2e-126 56
CbC4_1299 YP_004395976.1 tetanolysin O VFG0074 Protein 1e-117 49
CbC4_1299 YP_004395976.1 tetanolysin O VFG1363 Protein 5e-108 47