Gene Information

Name : Alide2_4500 (Alide2_4500)
Accession : YP_004390319.1
Strain : Alicycliphilus denitrificans K601
Genome accession: NC_015422
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 4718683 - 4720125 bp
Length : 1443 bp
Strand : -
Note : TIGRFAM: Pyruvate kinase; KEGG: dia:Dtpsy_3305 pyruvate kinase; PFAM: Pyruvate kinase, barrel; Pyruvate kinase, C-terminal-like

DNA sequence :
ATGTTCATGCGAAGCCGCCGCGCCACCAAAATCGTCGCCACCCTGGGCCCCGCCTCCAGCGACCCGCTGCTGCTGGAGCA
GATGATCGCGGCGGGCGTGAACGTGGTGCGCTTGAACTTCAGCCACGGCAAGGCGCGCGACCACGTGGAGCGCGCCGCCA
AGGTGCGCGAGGCCGCCCAGCGCGCCGGGCGCGAGGTGGCCATCATGGCCGACCTGCAGGGCCCCAAGATCCGCGTGGGC
AAGTTCGCCGAGGGGCGCGTGCACCTGGAGGTGGGGCAGCCGTTCGTCCTCGACGCCTCGCGCACCGAGCCCGGCGACCT
GCAGGGCGTGGGGCTGGACTACAAGGAGCTGCCGCGCGACGTGAGGGCGGGCGACGTGCTGCTGCTCAACGACGGCCTGA
TCGTGCTGCAGGTCGATGCGGTGCGCGGCGAGGCCGTGCACACCACGGTGAGGATAGGCGGCGAGCTGTCCAACAACAAG
GGCATCAACAAGCAGGGCGGGGGCCTGACGGCGCCGGCCCTCACGGCCAAGGACATGGAGGACATCAAGACGGCGATGAG
CTTCCAGGCCGACTACGTGGCCGTGAGCTTTCCCAAGAACGCCACCGACATGGAGATGGCGCGCCAGCTGTGCAACGTGG
CGGCCGCCGAGTTCCGCCACAAGCCCGGGCTGATCGCGAAGATAGAGCGCGCCGAGGCCATTCCGCGCCTCGAAGAGATC
CTGCACGTATCCGACGGCATCATGGTCGCGCGCGGCGATCTGGCCGTGGAGGTGGGCAACGCCGCCGTGCCGGCGCTGCA
GAAGAAGATGATCCGCATGGCGCGCGACATGGACAAGGTCGTGATCACCGCCACCCAGATGATGGAGAGCATGATCTCCA
ACCCCGTGCCCACGCGCGCCGAGGTCAGCGACGTGGCCAACGCCGTGCTCGACGGCACCGACGCCGTGATGCTCAGCGCC
GAGACGGCCGCCGGCAAGTATCCGCTGGAGACGGTGCAGGAGATGGCCAAGATCTGCGCCGCCGCCGAGGCCGCAGAGGA
CCCCGACCGCGACGCCGACTTCGACGGCCAGACGGCGGGCCGCGTCGACCAGTCCGTGGCCATGGGCGCGCTGTTCACCG
CCCACCACCTGGGCGCCAAGGCCATCGTGGCCATGACCGACAGCGGCTCCACGGCCCTGTGGATGAGCCGCCACCGCATC
CACATCCCGATCTACGCGCTGACGCCCAAGGTGGCCACGCAGCGGCGCATGGCCATGTTCCGCAATGTGCGCCCCCTGCT
CATGGACACCAGCGCCGACCGCGACACGGCCCTGGAGCAGGCCGAGAACCACCTGAAGATGCGCAACATCGTGCAGCAGG
GCGACCTGTACGTGATTACCTGCGGCGAGCCCATGGGCGCGCCGGGCGGCACGAACATGCTCAAGATCTGCCGGGCGCGG
TGA

Protein sequence :
MFMRSRRATKIVATLGPASSDPLLLEQMIAAGVNVVRLNFSHGKARDHVERAAKVREAAQRAGREVAIMADLQGPKIRVG
KFAEGRVHLEVGQPFVLDASRTEPGDLQGVGLDYKELPRDVRAGDVLLLNDGLIVLQVDAVRGEAVHTTVRIGGELSNNK
GINKQGGGLTAPALTAKDMEDIKTAMSFQADYVAVSFPKNATDMEMARQLCNVAAAEFRHKPGLIAKIERAEAIPRLEEI
LHVSDGIMVARGDLAVEVGNAAVPALQKKMIRMARDMDKVVITATQMMESMISNPVPTRAEVSDVANAVLDGTDAVMLSA
ETAAGKYPLETVQEMAKICAAAEAAEDPDRDADFDGQTAGRVDQSVAMGALFTAHHLGAKAIVAMTDSGSTALWMSRHRI
HIPIYALTPKVATQRRMAMFRNVRPLLMDTSADRDTALEQAENHLKMRNIVQQGDLYVITCGEPMGAPGGTNMLKICRAR

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pykA NP_273151.1 pyruvate kinase Not tested IHT-A Protein 3e-112 57
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 1e-60 41
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 7e-61 41
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 7e-61 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Alide2_4500 YP_004390319.1 pyruvate kinase VFG0479 Protein 3e-61 41