Gene Information

Name : Alide2_3377 (Alide2_3377)
Accession : YP_004389231.1
Strain : Alicycliphilus denitrificans K601
Genome accession: NC_015422
Putative virulence/resistance : Resistance
Product : mercuric reductase
Function : -
COG functional category : C : Energy production and conversion
COG ID : COG1249
EC number : 1.16.1.1
Position : 3517089 - 3518774 bp
Length : 1686 bp
Strand : -
Note : TIGRFAM: Mercury reductase, MerA; KEGG: mms:mma_1749 putative mercuric reductase; PFAM: FAD-dependent pyridine nucleotide-disulphide oxidoreductase; Heavy metal transport/detoxification protein; Pyridine nucleotide-disulphide oxidoreductase, dimerisation

DNA sequence :
ATGACCACCCTGAAGATCACCGGCATGACCTGCGATTCGTGCGCGGCCCACGTCAAGGACGCGTTGGAGAAGGTGCCGGG
CGTGCAGTCGGCCGTCGTGTCCTATGCCAAGGGCGCGGCGCAGGTCGCCATCAAACCCGGCACTGCATCGGACGTACTGA
CGGCCGCCGTGGCGGAGCTTGGCTACCAAGCGACGCTCGCCGATGCTCCGCTGACCGAGAATCGCAGCGGTCTGCTCGAC
AAGGTGCGCGGCTGGAGGGGGGCCGCCGACAAGCACAGCGATGGCGAACGCCCGTTGCAGGTTGCTGTGATCGGCAGCGG
CGGCGCGGCAATGGCGGCGGCGCTGAAAGCTGTCGAGCAAGGCGCACATGTCACGCTGATCGAGCGCGGCACGATCGGCG
GCACTTGCGTCAATGTGGGGTGCGTGCCGTCCAAGATCATGATCCGCGCCGCCCACATTGCGCATCTGCGCCGTGAAAGT
CCGTTCGACGGTGGCATCGCGGCGAATGTGCCCGCGATTGACCGCAGCAAGCTGCTGGCCCAGCAGCAGACGCGTGTTGA
TGAGCTGCGCCACGCCAAGTACGAAGGCATTCTGGACGGTAATCCGGCCATTACCGTACTGCACGGCGAAGCGCGTTTCA
AGGACGCCCAGAGTCTGACCGTGCGGCATGGCGATGGGGGTGAACGCGTGGTGAGTTTCAACCGCTGCCTAGTCGCCACC
GGTGCCAGTCCGGCCGTGCCGCCGATTCCGGGCTTGAAAGACACGCCGTATTGGACATCCACCGAGGCGCTGGTGAGTGA
CACCATTCCCGAGCGCCTGGCAGTGATCGGATCGTCCGTAGTCGCGCTGGAACTGGCGCAAGCCTTTGCCCGGCTGGGCA
GCCAGGTCACGATCCTGGCGCGCAACACTTTTTTCTTCCGCGAAGATCCGGCCATTGGTGAAGCCGTCACCGCAGCCTTC
CGTGCCGAAGGCCTCAAGGTGCTGGAACACACGCAGGCCAGCCAGGTCGCCCATGCAGACGGTGAATTCGTGCTGACCAC
CGGGCATGGTGAAGTGCGCGCCGACAGGTTGCTGGTCGCGACCGGCCGCACACCGAACACGCGCAGCCTGGCGCTGGAAG
CGGCGGGTGTCGCCACCAATGCGCAGGGGGCCATCGTCATCGACCAAGGCATGCGCACCAGCGCGCCGCATATCTATGCC
GCCGGCGACTGCACCGACCAGCCGCAATTCGTCTATGTGGCGGCGGCGGCCGGCACCCGTGCGGCCATCAACATGACCGG
CGGAGAAGCCACGCTCAATCTGACCGCGATGCCAGCAGTGGTGTTCACCGATCCGCAGGTGGCCACCGTGGGCTACAGCG
AAGCGCAAGCGCATCACGACGGCATCGGGATCGACAGCCGCACCTTGAGCCTGGACAACGTGCCACGCGCGCTGGCCAAC
TTCGATACGCGCGGTTTCATCAAGCTGGTGATTGAGAAAGGCAGCGGGCGACTCATCGGCGTGCAGGCGGTGGCTCCTGA
AGCGGGCGAGCTGATCCAGACGGCGGCGCTCGCGATCCGTAACCGCATGACGGTACAGGAACTGGCCGATCAGTTGTTTC
CCTATCTGACGATGGTCGAAGGGCTGAAGTTGGCGGCGCAGACCTTCAGTAAAGACGTGAAGCAGCTTTCGTGCTGCGCA
GGCTGA

Protein sequence :
MTTLKITGMTCDSCAAHVKDALEKVPGVQSAVVSYAKGAAQVAIKPGTASDVLTAAVAELGYQATLADAPLTENRSGLLD
KVRGWRGAADKHSDGERPLQVAVIGSGGAAMAAALKAVEQGAHVTLIERGTIGGTCVNVGCVPSKIMIRAAHIAHLRRES
PFDGGIAANVPAIDRSKLLAQQQTRVDELRHAKYEGILDGNPAITVLHGEARFKDAQSLTVRHGDGGERVVSFNRCLVAT
GASPAVPPIPGLKDTPYWTSTEALVSDTIPERLAVIGSSVVALELAQAFARLGSQVTILARNTFFFREDPAIGEAVTAAF
RAEGLKVLEHTQASQVAHADGEFVLTTGHGEVRADRLLVATGRTPNTRSLALEAAGVATNAQGAIVIDQGMRTSAPHIYA
AGDCTDQPQFVYVAAAAGTRAAINMTGGEATLNLTAMPAVVFTDPQVATVGYSEAQAHHDGIGIDSRTLSLDNVPRALAN
FDTRGFIKLVIEKGSGRLIGVQAVAPEAGELIQTAALAIRNRMTVQELADQLFPYLTMVEGLKLAAQTFSKDVKQLSCCA
G

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
merA ACN81005.1 MerA Hg(II) mercuric ion reductase Not tested AbaR5 Protein 0.0 88
merA CAJ77060.1 Mercuric ion reductase Not tested AbaR1 Protein 0.0 88
merA ABQ57371.1 MerA Not tested SGI1 Protein 0.0 87
merA AGK07021.1 MerA Not tested SGI1 Protein 0.0 87
merA AGK07079.1 MerA Not tested SGI1 Protein 0.0 87
merA YP_006098387.1 mercuric ion reductase Not tested Tn2411 Protein 0.0 84
merA AET25397.1 MerA Not tested PAGI-2(C) Protein 0.0 84
merA AFG30120.1 MerA Not tested PAGI-2 Protein 0.0 84
merA AAN62178.1 mercuric (Hg(II)) reductase Not tested PAGI-2(C) Protein 0.0 80
unnamed ABR13401.1 mercuric reductase Not tested PAGI-5 Protein 0.0 76
SE0085 NP_763640.1 dihydrolipoamide dehydrogenase Not tested SCCpbp4 Protein 3e-87 41
merA BAB47642.1 mercuric reductase Not tested Type-III SCCmec Protein 2e-87 41

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Alide2_3377 YP_004389231.1 mercuric reductase BAC0652 Protein 0.0 89
Alide2_3377 YP_004389231.1 mercuric reductase BAC0653 Protein 0.0 81
Alide2_3377 YP_004389231.1 mercuric reductase BAC0650 Protein 8e-174 69
Alide2_3377 YP_004389231.1 mercuric reductase BAC0651 Protein 1e-87 41