Gene Information

Name : Alide2_2051 (Alide2_2051)
Accession : YP_004387942.1
Strain : Alicycliphilus denitrificans K601
Genome accession: NC_015422
Putative virulence/resistance : Virulence
Product : N-6 DNA methylase
Function : -
COG functional category : V : Defense mechanisms
COG ID : COG0286
EC number : -
Position : 2144022 - 2146406 bp
Length : 2385 bp
Strand : -
Note : PFAM: DNA methylase, adenine-specific; KEGG: xfn:XfasM23_2177 N-6 DNA methylase

DNA sequence :
ATGCAGAAGAAACAACAACAAGACCAAAGCCAGATCAAGTGGATTTCCGACTTCATCTGGAACATCGCTGACGACCGTCT
GCGCGACGTTTACGTGCGCGGCAAGTACCGTGACGTGATCCTGCCTTTCACCGTGCTGCGACGGCTCGACGCCGTGCTCG
AAGCCACCAAGGACGCGGTGCTGGAGCGCAAGAAGTTCCTGGACACCCACAAGGTGGCCGAGCAGGACGGCGCGCTGCGC
ATGGCGGCAGGCCAGGCGTTCTACAACGTCTCGGAGTTCACGCTGGCCAAGCTGAAGGCCAGCGCGGCGGGGCAGCGGCT
GCGCGATGACTTCATTGCGTACCTGGACGGCTTCTCGCCCAACGTGCAGGAAATCCTCACCAAGTTCAACTTCCGCAACC
AGATCCAGAAGCTGGTGGATTCCCACGTCCTTGGCTACCTGATCGATGACTTCCTCGACCCCGAGGTCAATCTCGCGCCG
CTGCCGGTGAAGGATGCCGACGGCCGCATCAAGCTGCCCGCGCTGGACAACCACGGCATGGGCACGGTGTTCGAGGAGTT
AATCCGCCGCTTCAACGAAGACAACAATGAAGAGGCAGGCGAACACTTCACCCCGCGCGACGTGGTGCAGCTTATGGCCA
AACTGCTGTTCCTGCCGGTGGCCGACCGGATCGATTCCAGCACCTATTCGCTCTACGACGGCTCCTGCGGTACAGGCGGC
ATGCTCACCGTGGCCGAGGAAGCCTTGCATGAACTGGCAGAGGAACACGGCAAGGAAGTATCGATCCACCTGTTCGGGCA
GGAGATCAGCGACGAGACCTACGCCATCTGCAAGGCCGACCTGCTCTTGAAGGGCGAAGGCGCAGAGGCCGAGAACATCG
TCGGCGGCGCGGACAAGTCCACCCTGTCCGCCGACCAGTTCCGCAGCCGCGAATTCGATTTCATGATCTCCAACCCGCCC
TACGGCAAGAGTTGGAAGACCGATCTGGAGCGCATGGGAGGCAAGAAGGAATTCAACGACCCGCGCTTCATCGTCAGCCA
CGCGGGCAACGCCGAGTTCAAGCTCCTCACCCGTTCCAGCGACGGGCAGCTCATGTTCCAGGTGAACAAGCTGCAAAAGA
TGAAGCACAACACGCCGCTGGGCAGCCGCATCGCGCTGGTGCATAACGGCTCGGCGCTATTCACCGGCGACGCAGGCCAG
GGCGAGAGCAACATCCGCCGCTGGGTGCTGGAGAACGACTGGCTCGAAGCCATCATCGCCCTGCCGCTCAACATCTTCTA
CAACACCGGCATCGCCACCTACATCTGGGTGCTGGCCAACAAGAAGGCCGAAGCGCGCCGTGGCAAGGTGCAGTTGATCG
ACGCCAGCGGCTGGTTCCAGCCGCTGCGCCGCAACCTCGGCAAGAAGAACTGCGAACTGGCTGACGCCGACATTGCCCGC
ATCCTCGACCTGTATCTGGGCGAGGCGCAGGAAACCGCCCAGTCCAAATGGTTCGACACCCAGGACTTCGGCTACTGGAA
GATCACCGTCGAACGCCCGCTGCGCCTGAAAAGCCAGCTCTCCGACGAACGCATCGAGCCCCTGCGTTTTGCCACCGGCG
ACGAGGCGCTGCGCGCCGAGATCTACGCCACCCATGGCGACGCGCTCTACACCGAGTTTGCCAAGCGCAAACCGGCCATC
GAGGCGTGGCTGAAAGGCGAGGACGAGAACGAAGACGACGACAGCGAAGACAGCGACAGCGGCGATGACAGTGAGGCCCC
CGCAGCCCGCAAGGCCGTGCCCGCCAAGCGGCGCAAGAAGCTGCTAGACGCAACGACGTGGCAGCGCGACAAGGGGCTGA
TGGAAGTGGCGCAACGGGCGCAGCAGGCATTGGGCAGCGCCGTGTTCGACGACCACAATGAGTTCCGCACCCGCTTCGAT
GCTGCACTGAAGGCGCGGGGCGAAAAGCTCGGTGCGCCGGAGAAGAAGGCCATCTACAAGGCGGTGAGCTGGCGCGACGA
AGCAGCGCCGCCGGTCATCGCCAAGCGCAGCAAGCTCAAGGCGGGCGAACACTTTGAGCCCGGCTTCGATGGTGCGTACT
TGGAGACCGTGGGCAAGGATCGCTTCATGGTCGAGTACGAGCCCGACAGCGAGCTGCGCGACACCGAGCAGGTGCCATTG
AAGGAACCGGGCGGCATCGACGCCTTCTTTGCCCGCGAAGTATTGCCGCACGCGCCGGACGCCTGGATCGCCACGGATAA
GACCCAGATCGGCTACGAGATTTCGTTTGCCCGCTATTTCTACAAGCCCGCGCCGCTGCGCACGCTGGCAGAAATCCGCG
CCGACATCCTCGCGCTGGAGCAGCAAAGCGAAGGTTTGCTGCACAAGATCGTGGGGGGCGCGTGA

Protein sequence :
MQKKQQQDQSQIKWISDFIWNIADDRLRDVYVRGKYRDVILPFTVLRRLDAVLEATKDAVLERKKFLDTHKVAEQDGALR
MAAGQAFYNVSEFTLAKLKASAAGQRLRDDFIAYLDGFSPNVQEILTKFNFRNQIQKLVDSHVLGYLIDDFLDPEVNLAP
LPVKDADGRIKLPALDNHGMGTVFEELIRRFNEDNNEEAGEHFTPRDVVQLMAKLLFLPVADRIDSSTYSLYDGSCGTGG
MLTVAEEALHELAEEHGKEVSIHLFGQEISDETYAICKADLLLKGEGAEAENIVGGADKSTLSADQFRSREFDFMISNPP
YGKSWKTDLERMGGKKEFNDPRFIVSHAGNAEFKLLTRSSDGQLMFQVNKLQKMKHNTPLGSRIALVHNGSALFTGDAGQ
GESNIRRWVLENDWLEAIIALPLNIFYNTGIATYIWVLANKKAEARRGKVQLIDASGWFQPLRRNLGKKNCELADADIAR
ILDLYLGEAQETAQSKWFDTQDFGYWKITVERPLRLKSQLSDERIEPLRFATGDEALRAEIYATHGDALYTEFAKRKPAI
EAWLKGEDENEDDDSEDSDSGDDSEAPAARKAVPAKRRKKLLDATTWQRDKGLMEVAQRAQQALGSAVFDDHNEFRTRFD
AALKARGEKLGAPEKKAIYKAVSWRDEAAPPVIAKRSKLKAGEHFEPGFDGAYLETVGKDRFMVEYEPDSELRDTEQVPL
KEPGGIDAFFAREVLPHAPDAWIATDKTQIGYEISFARYFYKPAPLRTLAEIRADILALEQQSEGLLHKIVGGA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
VC0395_A1367 YP_001217310.1 DNA methylase HsdM Not tested VPI-2 Protein 1e-152 46
VC1769 NP_231404.1 DNA methylase HsdM Not tested VPI-2 Protein 1e-152 46
VPI2_0017c ACA01834.1 DNA methylase HsdM Not tested VPI-2 Protein 2e-152 45

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Alide2_2051 YP_004387942.1 N-6 DNA methylase VFG1102 Protein 5e-153 46