Gene Information

Name : Alide2_1949 (Alide2_1949)
Accession : YP_004387842.1
Strain : Alicycliphilus denitrificans K601
Genome accession: NC_015422
Putative virulence/resistance : Resistance
Product : mercuric reductase
Function : -
COG functional category : C : Energy production and conversion
COG ID : COG1249
EC number : 1.16.1.1
Position : 2048384 - 2050069 bp
Length : 1686 bp
Strand : -
Note : TIGRFAM: Mercury reductase, MerA; KEGG: bxe:Bxe_C1213 putative mercuric reductase; PFAM: FAD-dependent pyridine nucleotide-disulphide oxidoreductase; Heavy metal transport/detoxification protein; Pyridine nucleotide-disulphide oxidoreductase, dimerisation

DNA sequence :
ATGACCACCCTGAAAATCACCGGGATGACCTGCGACTCGTGCGCGGCTCACGTCAAGGAAGCCTTGGAGAAAGTGCCCGG
CGTGCAATCGGCGCTGGTGTCCTATCCGAAGGGCACAGCGCAACTCGCCATTGAGGCGGGCACGTCATCGGATGCGCTGA
CTACCGCCGTGGCCGGACTGGGCTACGAGGCAACGCTTGCCGATGCGCCACCGACGGACAACCGCGCCGGCCTGCTCGAC
AAGATGCGCGGCTGGATAGGGGCCGCTGATAAGCCCAGTGGCAACGAACGCCCGTTGCAGGTCGTCGTCATTGGTAGCGG
TGGCGCGGCAATGGCGGCAGCACTGAAGGCCGTCGAGCAAGGCGCGCACGTCTCGCTGATCGAGCGCGGCACCATCGGCG
GCACCTGCGTCAACGTCGGCTGCGTGCCGTCCAAGATCATGATTCGCGCCGCCCACATCGCCCATTTACGTCGGGAAAGT
CCGTTCGACGGCGGTATCGCGGCGACTGTGCCTGCGATTGACCGCAGCAAACTACTGGCCCAGCAGCAGGCGCGCGTCGA
AGAACTGCGCCATGCCAAGTACGAAGGCATTCTGGACGGCAACCCGGCCATCACCGTTGTACATGGTGAGGCGCGTTTCA
AGGATGAGCAGAGCCTGGTCGTCCGTTTAAACGATGGTGGCGAGCGCGTGGTAGCTTTCGACCGCTGCTTAGTCGCCACG
GGTGCCAGCCCGGCCGTGCCGCCGATTCCGGGCCTGAAAGAGTCACCCTACTGGACTTCGACCGAGGCCCTGGTCAGCGA
CACCCTTCCCGAACGCCTAGCCGTGATCGGCTCGTCGGTGGTCGCGCTGGAACTGGCGCAAGCCTTCGCCCGGCTGGGCA
GCCAGGTCACGATCCTAGCTCGCAACACGCTGTTCTTCCGCGACGACCCGTCCATCGGCGAGGCCGTCACAGCGGCCTTC
CGTGCCGAAGGGATCAAGGTACTGGAACACACGCAAGCCAGCCAGGTCGCGCATGTGAACGGCGAATTCGTGCTGACCAC
GGGACACGGTGAAGTACGTGCCGACAAGCTGCTGGTCGCTACCGGACGGACACCGAACACGCGCAGCCTGGCACTCGACG
CGGCGGGAGTCACCGTCAATGCGCAGGGTGCCATCGTCATCGACAAGGGCATGCGCACCAGCACGCCACACATTTACGCG
GCCGGCGACTGCACGGACCAGCCACAATTCGTCTATGTGGCGGCAGCGGCCGGCACCCGTGCCGCGATCAACATGACCGG
AGGGGATGCTGCCATCAATCTAACCGCGATGCCGGCCGTGGTGTTCACCGATCCGCAAGTAGCAACCGTGGGCTACAGCG
AAGCGGAAGCGCATCACGACGGGATCGAAACCGACAGTCGCACGCTCACGCTCGACAACGTGCCGCGTGCGCTTGCCAAC
TTCGACACACGCGGCTTTATCAAGCTGGTCATCGAGGAAGGTAGCGGACGGCTCATCGGCGTGCAGGTCGTCGCCCCGGA
AGCCGGCGAATTGATCCAGACGGCTGTTCTCGCCATTCGCAACCGCATGACGGTGCAGGAACTGGCCGACCAGTTGTTCC
CCTACCTGACGATGGTCGAGGGGTTGAAGCTCGCGGCGCAGACCTTCAGCAAGGACGTGAAGCAGCTTTCGTGTTGTGCC
GGATGA

Protein sequence :
MTTLKITGMTCDSCAAHVKEALEKVPGVQSALVSYPKGTAQLAIEAGTSSDALTTAVAGLGYEATLADAPPTDNRAGLLD
KMRGWIGAADKPSGNERPLQVVVIGSGGAAMAAALKAVEQGAHVSLIERGTIGGTCVNVGCVPSKIMIRAAHIAHLRRES
PFDGGIAATVPAIDRSKLLAQQQARVEELRHAKYEGILDGNPAITVVHGEARFKDEQSLVVRLNDGGERVVAFDRCLVAT
GASPAVPPIPGLKESPYWTSTEALVSDTLPERLAVIGSSVVALELAQAFARLGSQVTILARNTLFFRDDPSIGEAVTAAF
RAEGIKVLEHTQASQVAHVNGEFVLTTGHGEVRADKLLVATGRTPNTRSLALDAAGVTVNAQGAIVIDKGMRTSTPHIYA
AGDCTDQPQFVYVAAAAGTRAAINMTGGDAAINLTAMPAVVFTDPQVATVGYSEAEAHHDGIETDSRTLTLDNVPRALAN
FDTRGFIKLVIEEGSGRLIGVQVVAPEAGELIQTAVLAIRNRMTVQELADQLFPYLTMVEGLKLAAQTFSKDVKQLSCCA
G

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
merA ACN81005.1 MerA Hg(II) mercuric ion reductase Not tested AbaR5 Protein 0.0 94
merA CAJ77060.1 Mercuric ion reductase Not tested AbaR1 Protein 0.0 94
merA ABQ57371.1 MerA Not tested SGI1 Protein 0.0 90
merA AGK07021.1 MerA Not tested SGI1 Protein 0.0 90
merA AGK07079.1 MerA Not tested SGI1 Protein 0.0 90
merA YP_006098387.1 mercuric ion reductase Not tested Tn2411 Protein 0.0 86
merA AET25397.1 MerA Not tested PAGI-2(C) Protein 0.0 86
merA AFG30120.1 MerA Not tested PAGI-2 Protein 0.0 86
merA AAN62178.1 mercuric (Hg(II)) reductase Not tested PAGI-2(C) Protein 0.0 80
unnamed ABR13401.1 mercuric reductase Not tested PAGI-5 Protein 0.0 75

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Alide2_1949 YP_004387842.1 mercuric reductase BAC0652 Protein 0.0 92
Alide2_1949 YP_004387842.1 mercuric reductase BAC0653 Protein 0.0 80
Alide2_1949 YP_004387842.1 mercuric reductase BAC0650 Protein 1e-176 69
Alide2_1949 YP_004387842.1 mercuric reductase BAC0224 Protein 3e-85 41