Gene Information

Name : Clole_2583 (Clole_2583)
Accession : YP_004309484.1
Strain : Clostridium lentocellum DSM 5427
Genome accession: NC_015275
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 2825727 - 2827496 bp
Length : 1770 bp
Strand : -
Note : TIGRFAM: pyruvate kinase; KEGG: csc:Csac_1831 pyruvate kinase; PFAM: pyruvate kinase, barrel; pyruvate kinase, C-terminal-like; PEP-utilising enzyme, mobile domain

DNA sequence :
ATGCGCAAAACTAAAATTATTTGTACATTAGGACCTAAGACCAGAGACGTAGACAATATAAGAAAAATCATCGAAACAGG
TGCTGATGCGATTCGTATCAATTTTTCACATGATGATCATAAAATTCATGGAGAAACCGTTAAACGTGTTATTAAAGTAA
GAGAAGAGCTTGGAAAACCTATTCCTCTTATTCTAGATACTAAAGGTCCAGAAATTAGAACTGGTGTCATGAAAGATGAC
TTAGACCATAAGTTAAACATTGGAGATAGTTTTACTTTAACAACAGAGGAAATTGAAGGTGACGGCAAGCGTGTAAGTGT
GACTTATAAAAATTTGCCTTTTGATTTAAAAAGAGGTAGTCGTATTTTAATTGACGATGGTCTCATTGAACTCCGTGTCA
AAAATTTGACGGATACTGACATTGAATGTAGTGTCGTTAATGGTGGTGTATTAGGTTCAAGAAAAGGGGTTAATATTCCT
GATGTATTTGTTAATTTACCTGCACTTACAGAAAAAGATATTGCAGACGTAGAGTTTGCACCAAAAGCAGGCTTTGACTA
TATTGCTGCCTCTTTTATCCGTTGTGCTGCTGATATTATTAAAATTCGTCAAGTGCTAGAAGAAGCAGGCGGAAGTGATA
TTCAAATTATTGCTAAAATCGAAAATCGAGATGGTGTCAATAACATCGATGAAATCATCGAAGTAGCAGATGCAATCATG
GTTGCTAGAGGTGACTTAGGTGTAGAAATTCCTACAGAAGAAGTACCTATCGTGCAAAAAGAACTTATTAAAAAAGCAAA
TAAAGCAGGTAAACCTGTTATTACAGCAACACAAATGCTAGATTCTATGATTCGTAATCCTAGACCTACAAGAGCAGAAA
CAACCGACGTAGCTAATGCTATTTTTGATGGTACGAGTGCTATCATGCTTTCAGGAGAAACAGCTAAAGGGGATTATCCT
TTAGAAGCTATTCAAACGATGGCTAAGATTGCTATGACAGCAGAAGCTGTTGTAGCGAAACAAGAACAGGAAGCTTTAGT
GAGTAAAAACTATGGTATTAGTATGACTAATGCAGTAAGTCATGCAACCTGTAATACTGCTAGAGAATTAGATGCTGCAT
GTATTATTAGTATTACGAAATCCGGCTATACAGCACGTTCTATTTCTAAATTTAAACCTAACTGTCGTATTGTAGGTTGT
ACCAATAATCAAAGAGTACTTAGACAGCTTAACTTAGTTTGGGGTTGTACGCCTGTACTGATTACACCTAAAGAAGGGGC
TTCTACAGATGAAACTTTTGCATTAGCGGTTCAAAAAGTTGAAGAGCTTAAACTTGCTGAGCAAGGTGATGTAGTGGTTT
TATCAACAGGGGTACCATTAGGGGCAGAAGGTACAAGTAATATTATGAAGGTTCAATACGTGGGAAACGTACTTACTAAA
GGTGTGGGTTTCGGCAATAAGTCTATCACAGCACGCGCCAGTGTGATTAAAGTATTAGACGAAGCGGATCAATATTTTAA
ACAAGGAGATATTTTAGTAGCTCATAAAACGAGCAACGAATATTTACCTTATATGAAAAAAGCAAGTGCTATTGTTGTAG
AAGAAAGTGCACTTGAAGAAAATAATCATGCCGTAATTGTTGGGAGAACTTTAGATATTCCCGTTATTATTGGGGCTAAT
GGGATTATAGAAGGACTTAAAAATGCTACTGTTATTACTGTAGATGGTAGAAATGGCTATATCTATAATGGCGTAGTACA
TAAAGCATAA

Protein sequence :
MRKTKIICTLGPKTRDVDNIRKIIETGADAIRINFSHDDHKIHGETVKRVIKVREELGKPIPLILDTKGPEIRTGVMKDD
LDHKLNIGDSFTLTTEEIEGDGKRVSVTYKNLPFDLKRGSRILIDDGLIELRVKNLTDTDIECSVVNGGVLGSRKGVNIP
DVFVNLPALTEKDIADVEFAPKAGFDYIAASFIRCAADIIKIRQVLEEAGGSDIQIIAKIENRDGVNNIDEIIEVADAIM
VARGDLGVEIPTEEVPIVQKELIKKANKAGKPVITATQMLDSMIRNPRPTRAETTDVANAIFDGTSAIMLSGETAKGDYP
LEAIQTMAKIAMTAEAVVAKQEQEALVSKNYGISMTNAVSHATCNTARELDAACIISITKSGYTARSISKFKPNCRIVGC
TNNQRVLRQLNLVWGCTPVLITPKEGASTDETFALAVQKVEELKLAEQGDVVVLSTGVPLGAEGTSNIMKVQYVGNVLTK
GVGFGNKSITARASVIKVLDEADQYFKQGDILVAHKTSNEYLPYMKKASAIVVEESALEENNHAVIVGRTLDIPVIIGAN
GIIEGLKNATVITVDGRNGYIYNGVVHKA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 4e-87 47
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 1e-87 47
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 1e-87 47
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 9e-87 47

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Clole_2583 YP_004309484.1 pyruvate kinase VFG0479 Protein 5e-88 47