Gene Information

Name : Clole_2251 (Clole_2251)
Accession : YP_004309158.1
Strain : Clostridium lentocellum DSM 5427
Genome accession: NC_015275
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 2475216 - 2476955 bp
Length : 1740 bp
Strand : -
Note : TIGRFAM: pyruvate kinase; KEGG: cpy:Cphy_0741 pyruvate kinase; PFAM: pyruvate kinase, barrel; pyruvate kinase, C-terminal-like; PEP-utilising enzyme, mobile domain

DNA sequence :
ATGAGAAAAACGAAAATAGTTTGTACACTAGGACCTGCTACAGATGATGAACAAATATTAAGAGAGTTAATGCTTGAAGG
TATGAATGTTGCGCGTATCAATTTTTCACATGGTACTTACGAAAAACATCAACAAACTATTTCATTAGTGAAAAAGTTAA
GACAAGAACTGAATTTACCAGTTGCTTTATTATTAGATACAAAAGGGCCTGAAATTCGTATTGGTGATTTTGAGGAAAGA
GAAGTAGAGCTTAAAAAGGATCAAGTTTTTACATTGACAACAGAAGCACTTAAAGGAAATGAAACAAAAGTATCAATTAC
CTATAACGAACTTATTAAGGATGTTTCAGAAGGCATGACGATTTTATTAGATGATGGGCTTATTGAACTTAAAGTAACCA
GTAAAACAAATAAAGATATTATTTGTCATGTTGAAAATGGGGGAATTTTATCTAGTCATAAGGGAGTTAATATTCCAGGG
GCTCATTTATCTATGCCTTTTATCAGCAAGCAAGATTACTCAGATATTGTATTTGGAATTGAGCAAGGTGTAGACTTTAT
AGCCGCTTCTTTCACAAGAACAGCTGAGGATATTATACAGATTCGGAAAATACTAAGTGATTATCAATGTAATACCATTA
ATATTATTGCTAAGATAGAAAATAGACAAGGTATTGAAAATATAGATGATATTATTCGTGTATCAGATGGTATTATGGTA
GCCAGAGGAGATTTAGGGGTAGAAGTACCTATTGAAGAAATTCCAGTTATTCAGAAGAAAATTATTAGCAAGGTATGTAA
TGCAGAGAAACCTGTTATTACTGCTACACAGATGTTAGATTCTATGATGAAAAATCCTAGACCTACACGTGCAGAAGTTA
CAGATGTTGCCAATGCTATTTATGATGGAACCAGTGCTATTATGTTATCAGGCGAAACAGCGGCAGGTAAATATCCAGTT
GAAGCATTACAAACAATGATTAAAATTACAGAAAGAGCAGAACAAGATATTAATTATAGACAGCGTTTTAAAAATAGAGA
TTGTTTGACTAATCCCGATGTAACTAACGCTATTTCTCATGCTACTTGCACAACTGCACATGATATTAATGCAGCAGCTA
TTATTACAGTAACAAAATCTGGTAAAACTGCTCGAATGATTGCAAAGTATAAACCGGATTGTCCCATTATTGGTTGTAGC
ACATATGAATATGTTTGTAGACAAATTAATTTATCATGGGGTGTCATGCCATTACTTATAAAAGAAGAAAAAAATACAGA
TGACTTATTTGAGCATGCTGCTGAAGCAGTTGAAAGAGTAGGCTATGTAAAGCCAGGAGAAATTGTGGTATTAACAGCCG
GAGTACCTTTAGGTATAGCTGGAACGACCAATCTTATTAAGGTACAAGTCATAGGACATATTCTTTTAAAGGGGAGAGGA
ATGATTCATAAGACTGTTTGTGCCAGCTTATGTGTTTGTAAAGATGAAGAAGAAGTGAAAAATGATTTTAAACCGGGAGA
TATTTTAGTAATGCCTAAAACAAGTAATCATATTATAGAAGAACTTAAACAAGCTGCTGGCTTAATTATAGAAGAAGATG
GCATGAATTCACACGCGGCTATTGTTAGTTTAAGCTTAGACATTCCTATTATTTTGGGGGCACAGGGAGCAACTGAAATA
TTAAAATCTGGAGCAATTGTCACATTAGATGGAGAAACGGGCGTAGTTTCATGTAATTAA

Protein sequence :
MRKTKIVCTLGPATDDEQILRELMLEGMNVARINFSHGTYEKHQQTISLVKKLRQELNLPVALLLDTKGPEIRIGDFEER
EVELKKDQVFTLTTEALKGNETKVSITYNELIKDVSEGMTILLDDGLIELKVTSKTNKDIICHVENGGILSSHKGVNIPG
AHLSMPFISKQDYSDIVFGIEQGVDFIAASFTRTAEDIIQIRKILSDYQCNTINIIAKIENRQGIENIDDIIRVSDGIMV
ARGDLGVEVPIEEIPVIQKKIISKVCNAEKPVITATQMLDSMMKNPRPTRAEVTDVANAIYDGTSAIMLSGETAAGKYPV
EALQTMIKITERAEQDINYRQRFKNRDCLTNPDVTNAISHATCTTAHDINAAAIITVTKSGKTARMIAKYKPDCPIIGCS
TYEYVCRQINLSWGVMPLLIKEEKNTDDLFEHAAEAVERVGYVKPGEIVVLTAGVPLGIAGTTNLIKVQVIGHILLKGRG
MIHKTVCASLCVCKDEEEVKNDFKPGDILVMPKTSNHIIEELKQAAGLIIEEDGMNSHAAIVSLSLDIPIILGAQGATEI
LKSGAIVTLDGETGVVSCN

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 4e-94 47
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 6e-93 47
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 5e-94 47
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 4e-94 47

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Clole_2251 YP_004309158.1 pyruvate kinase VFG0479 Protein 2e-94 47