Gene Information

Name : Clole_1021 (Clole_1021)
Accession : YP_004307951.1
Strain : Clostridium lentocellum DSM 5427
Genome accession: NC_015275
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 1172749 - 1174491 bp
Length : 1743 bp
Strand : +
Note : TIGRFAM: pyruvate kinase; KEGG: cpy:Cphy_0741 pyruvate kinase; PFAM: pyruvate kinase, barrel; pyruvate kinase, C-terminal-like; PEP-utilising enzyme, mobile domain

DNA sequence : 
ATGAGAAAAACTAAAATTGTATGTACATTAGGTCCATCAACAAAGGATGACAAAATACTAAAGAGATTAATGCTAGAAGG
GATGGATGTTGCTAGATTCAACTTCTCACATGGCACTCACGAAAGTCATAAAGAAAATTTTGACCGTGTAGATAAACTTA
GAAAAGAATTAGATTTACCAATAGCAGTTTTACTTGATACAAAAGGACCAGAAGTAAGAGTTTGTCAATTTGCAGAAGGA
AAAGTTTCTCTTAAAAAAGGTGAACAATTTACACTTACAAGTAGAGATATTCTAGGAACAAATGAAATTGTTTCTATCAC
ATACAAGAATTTACCAAATGATGTAAAAGAAGGTTCAAGAATTTTACTTGATGATGGTCTTATTGAGTTAATCGTGGAAA
AAGTAGTAGACGGCAGTGATATTGTTTGTAAAATTATGAATAATGGTGATGTATCAAACAATAAAGGTGTTAACTTACCA
GATACTAGACTTTCTATGCCATACTTAAGTGAAAAAGATCGATCTGATATCATCTTTGGAATTCAAACAGGTTATGACTT
TATTGCAGCTTCTTTTGTAAGATGCGCCGAAGATGTTCTTCAAATTAGAAGAATTCTTGAAGAATATAATTGTCATACAA
TTAATATTATCTCTAAAATTGAGAATCAAGAAGGTGTAGATAATATTGATGAAATTATTCGTGTATCTGATGGAATTATG
GTAGCCCGTGGAGATATGGGGGTTGAAATTCCAGGAGAAGAAGTACCATCTATCCAAAAAATGATTATTAAGAAAACAGT
TGCAGCAGGTAAACAAGTTATTACTGCTACACAAATGTTAGATTCCATGATGAAAAATCCTAGAGCTACAAGAGCCGAAA
TTAGTGATGTTGCTAATGCAATCTACGATGGTACAAGTGCTATTATGTTATCTGGTGAAACAGCAGCAGGTGCATATCCT
GTTGAATCAGTACAAACGATGGCGAGAATTGCTTTAAAAACAGAGTCAGATATTGATTATGTAAAACGTTTTAAAGAAAG
AGGTAGTACAGCAACTAATAATGTAACTAGTGCAATCTCACATGCTACTTGTACAACAGCACATGACCTTGGTGCAGCAG
CTATTGTAACTGTAACTAAGTCAGGTAGAACAGCTAGAATGATTTCTAAATATAGACCAGTATCACCTATCTTGGGTTGC
ACAACTTCAGCAAGTGTATACCGTCACATGAGTTTAATGTGGGGTGTAAAACCACTTTTAATGGAAGAAAAACAAAATGC
AGATGAATTATTTGAAGAAGCATTAACTGTAGCTGAACACACAGGTATGGTTAATAAAGGGGAATTAGTTGTTATCACAA
GTGGCTTACCAGTAGGCGTAACCGGTACAACTAATATGATTAAGGTAGATGTCATCGGACACATTTTAGTTTCAGGTGTA
GGTGTTGGTTCAAAACAAGTATGTGGTAATCTTTGCGTATGTAAAACAGAAGAAGAAGCTAATGAGAAATTTATTGAAGG
GGATATCCTTGTTATTCCGGAAACTACAAATGAGATGCTACCATTATTAAGAAGAGCAGCTGGTATTATTACAGAAGAAG
ATGGTGTTAATTCACATGCTGCAATTGTTGGACTTGCTATTGATATTCCAGTCATTACTGGTGCGACAAATGCAACAGAT
ATTCTTAAAACAGGTTCAGTTGTAAGCCTAGATGCAGGTAGAGGAATTGTTTCAAGTAACTAA

Protein sequence : 
MRKTKIVCTLGPSTKDDKILKRLMLEGMDVARFNFSHGTHESHKENFDRVDKLRKELDLPIAVLLDTKGPEVRVCQFAEG
KVSLKKGEQFTLTSRDILGTNEIVSITYKNLPNDVKEGSRILLDDGLIELIVEKVVDGSDIVCKIMNNGDVSNNKGVNLP
DTRLSMPYLSEKDRSDIIFGIQTGYDFIAASFVRCAEDVLQIRRILEEYNCHTINIISKIENQEGVDNIDEIIRVSDGIM
VARGDMGVEIPGEEVPSIQKMIIKKTVAAGKQVITATQMLDSMMKNPRATRAEISDVANAIYDGTSAIMLSGETAAGAYP
VESVQTMARIALKTESDIDYVKRFKERGSTATNNVTSAISHATCTTAHDLGAAAIVTVTKSGRTARMISKYRPVSPILGC
TTSASVYRHMSLMWGVKPLLMEEKQNADELFEEALTVAEHTGMVNKGELVVITSGLPVGVTGTTNMIKVDVIGHILVSGV
GVGSKQVCGNLCVCKTEEEANEKFIEGDILVIPETTNEMLPLLRRAAGIITEEDGVNSHAAIVGLAIDIPVITGATNATD
ILKTGSVVSLDAGRGIVSSN

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 6e-93 47
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 3e-93 47
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 3e-93 47
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 2e-92 47
pykA NP_273151.1 pyruvate kinase Not tested IHT-A Protein 7e-60 41

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Clole_1021 YP_004307951.1 pyruvate kinase VFG0479 Protein 1e-93 47