Gene Information

Name : Sgly_0598 (Sgly_0598)
Accession : YP_004264962.1
Strain : Syntrophobotulus glycolicus DSM 8271
Genome accession: NC_015172
Putative virulence/resistance : Virulence
Product : type III secretion system ATPase, FliI/YscN
Function : -
COG functional category : N : Cell motility
COG ID : COG1157
EC number : 3.6.3.15
Position : 587289 - 588602 bp
Length : 1314 bp
Strand : +
Note : COGs: COG1157 flagellar biosynthesis/type III secretory pathway ATPase; InterPro IPR004100: IPR000194: IPR005714: IPR003593; KEGG: dsy:DSY2986 hypothetical protein; PFAM: ATPase, F1/V1/A1 complex, alpha/beta subunit, nucleotide-binding domain; ATPase, F1/

DNA sequence :
ATGGCGGATTGGACAAACCTGGCCAATAAACTGAAGGATATGGAAACGGTTACGGCGCTGGGGCTGGTATCGAAAGTTAC
CGGTCTGCTGATTGAAGCCAATGGGCCAAAGGTAAGTGTCGGAAGATATTGCTGTATTGTATCTTCCAAATCGGAGATCC
CCGCCGAGGTAGTCGGATTTAAAGATAATAAGACCCTGTTGATGCCTCTGGGGGAATTGGAGGGAGTATCCCCGGGCGAC
AAGGTGATTCCTCAGAAGGAGAAGCTTGCCGTTAAAGTGGGATCAGAGCTTTTGGGGCGCGTTCTTGACGGATTGGGAAA
CCCTCTGGAAGGAAGGCCCTTGAAATCAAATATTGATTATCCTCTGCAAAATGCTCCTCCGCATGCTTTGCTGAGACCTC
GTGTCAGTGAACCGCTCAGTGTTGGGGTAAGATCGGTTGACGGTCTGCTGACAATAGGGAGAGGCCAGAGAATGGGCATA
TTTGCCGGTTCCGGTGTCGGGAAGAGTACCCTTCTGGGTATGATGGCCCGCAATACACAAGCGGATATTAATGTGATCGC
TCTGGTGGGAGAACGGGGAAGAGAGCTTCGGGAATTCATTGAGAAGGATTTAGGTCCGGAGGGAATAAGCCGCTCAGTGA
TTGTGGTTGCCACATCTGACCAGCCGGCTTTAGTAAGGATCAAAGCGGCGTTTACTGCTACAGCTATTGCTGAATATTTT
CGGGATACGGGAAAAAATGTCCTGCTAATGATGGATTCGGTCACACGCTTTGCCATGGCCCAGAGAGAAGTGGGCCTGAC
AATTGGAGAGCCGCCCGCAACAAGAGGATATCCCCCTTCAGTGTTCGCGATGCTCCCCAAGCTTTTGGAAAGAGCGGGGA
TGGCGGAAAAGGGCAGCATCACAGGTTTATATACCGTGCTGGTTGACGGAGATGATCACAATGAGCCGATCGCTGATGCG
GTGAGAGGGATTTTAGACGGACATATCGTCTTATCCAGGGATCTTGCGGCAAGAAATCATTTTCCTTCTATTGATATTTT
GCAATCTGTTTCCAGATGTATGAGTGATGTGGCGGAACCGGAGATGATCGGGCTGGCGGGAAAAGTACGCGAACTGCTGG
CGGTTTATCAAGGAGCGAAAGACCTGATCGACATCGGGGCTTATGTCCGGGGAAGTAATGCCAAGATTGATACTGCTTTA
AAATATATGGAAAAGATCACCCTGTTTTTATGCCAAAGTACGGATGAAAAATTTAATGGTATTGATATGGTTGCGGAAAT
CAAGAAAATTTTTACCGAAGAGGGTAGATCATGA

Protein sequence :
MADWTNLANKLKDMETVTALGLVSKVTGLLIEANGPKVSVGRYCCIVSSKSEIPAEVVGFKDNKTLLMPLGELEGVSPGD
KVIPQKEKLAVKVGSELLGRVLDGLGNPLEGRPLKSNIDYPLQNAPPHALLRPRVSEPLSVGVRSVDGLLTIGRGQRMGI
FAGSGVGKSTLLGMMARNTQADINVIALVGERGRELREFIEKDLGPEGISRSVIVVATSDQPALVRIKAAFTATAIAEYF
RDTGKNVLLMMDSVTRFAMAQREVGLTIGEPPATRGYPPSVFAMLPKLLERAGMAEKGSITGLYTVLVDGDDHNEPIADA
VRGILDGHIVLSRDLAARNHFPSIDILQSVSRCMSDVAEPEMIGLAGKVRELLAVYQGAKDLIDIGAYVRGSNAKIDTAL
KYMEKITLFLCQSTDEKFNGIDMVAEIKKIFTEEGRS

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
lscN AAO18044.1 LscN Virulence TTSS locus Protein 2e-101 55
XC_3006 YP_244074.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-98 54
hrcN NP_636610.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-98 54
hrpB6 BAB07852.1 HrpB6 Virulence Hrp PAI Protein 4e-98 53
hrpB6 AAF61281.1 HrpB6 Virulence Hrp PAI Protein 4e-98 53
hrcN YP_362163.1 type III secretion system ATPase Virulence Hrp PAI Protein 3e-97 53
hrcN YP_198730.1 type III secretion system ATPase Virulence Hrp PAI Protein 5e-98 53
hrpB6 AAB08461.1 HrpB6 Virulence Hrp PAI Protein 2e-97 53
hrcN AAP34339.1 HrcN Virulence Hrp PAI Protein 9e-98 52
hrcN NP_640767.1 type III secretion system ATPase Virulence Hrp PAI Protein 4e-97 52
escN YP_003232153.1 T3SS structure protein EscN Virulence LEE Protein 1e-86 51
unnamed AAL06369.1 EscN Virulence LEE Protein 1e-84 51
escN AAK26715.1 EscN Virulence LEE Protein 1e-86 50
ECs4568 NP_312595.1 EscN Virulence LEE Protein 5e-86 50
escN AAL57542.1 EscN Virulence LEE Protein 1e-86 50
escN CAC81862.1 EscN protein Virulence LEE II Protein 9e-87 50
escN YP_003236088.1 translocator EscN Virulence LEE Protein 2e-86 50
escN CAI43874.1 EscN protein Virulence LEE Protein 6e-87 50
escN YP_003223475.1 translocator EscN Virulence LEE Protein 8e-87 50
escN AAC31513.1 L0034 Virulence LEE Protein 4e-86 50
unnamed ACU09458.1 type III secretion apparatus H+-transporting two-sector ATPase Virulence LEE Protein 4e-86 50
escN AAC38383.1 EscN Virulence LEE Protein 2e-86 50
escN NP_290268.1 hypothetical protein Virulence LEE Protein 5e-86 50
escN AFO66300.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-83 50
escN AFO66415.1 putative LEE-encoded type III secretion system ATPase Virulence SESS LEE Protein 1e-83 50
ssaN YP_216423.1 type III secretion system ATPase Virulence SPI-2 Protein 7e-90 49
ssaN CAA70537.1 secretion system apparatus Virulence SPI-2 Protein 1e-89 49
ssaN NP_456113.1 putative type III secretion ATP synthase Virulence SPI-2 Protein 9e-89 49
ssaN NP_805086.1 type III secretion system ATPase Virulence SPI-2 Protein 9e-89 49
ssaN NP_460380.1 type III secretion system ATPase Virulence SPI-2 Protein 2e-89 49
YPO0267 YP_002345348.1 type III secretion system ATPase Virulence Not named Protein 3e-86 49
hrcN AAT96299.1 HrcN Virulence S-PAI Protein 5e-81 48
hrcN AAT96258.1 HrcN Virulence S-PAI Protein 1e-80 48
hrcN AAT96339.1 HrcN Virulence S-PAI Protein 5e-81 48
hrcN AAB06001.1 HrcN Virulence Hrp PAI Protein 4e-84 47
hrcN ABA47275.1 HrcN Virulence S-PAI Protein 3e-87 47
hrcN AAT96141.1 HrcN Virulence T-PAI Protein 7e-76 46
hrcN AAT96195.1 HrcN Virulence T-PAI Protein 5e-76 46
hrcN AAR25633.1 type III secretion cytoplasmic ATPase HrcN Not tested Not named Protein 9e-75 45
hrcN AAG33879.1 HrcN Virulence Hrp PAI Protein 2e-71 45
hrcN NP_791227.1 type III secretion cytoplasmic ATPase HrcN Virulence Hrp PAI Protein 3e-74 44
hrcN ABQ88354.1 HrcN Virulence Hrp PAI Protein 8e-70 44
hrpJ4 AAA81942.1 HrpJ4 Virulence Hrp PAI Protein 8e-70 44
invC AAS66861.1 InvC Not tested SSR-2 Protein 1e-69 43
invC YP_217813.1 ATP synthase SpaL Virulence SPI-1 Protein 6e-71 42
spaI NP_457288.1 secretory apparatus ATP synthase (associated with virulence) Virulence SPI-1 Protein 6e-71 42
spaI NP_806497.1 ATP synthase SpaL Virulence SPI-1 Protein 6e-71 42
invC NP_461815.1 ATP synthase SpaL Virulence SPI-1 Protein 6e-71 42
ECs3730 NP_311757.1 ATP synthase SpaL Not tested LIM Protein 3e-69 42
ysaN AAS66842.1 YsaN Not tested SSR-1 Protein 4e-68 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Sgly_0598 YP_004264962.1 type III secretion system ATPase, FliI/YscN VFG2144 Protein 1e-105 54
Sgly_0598 YP_004264962.1 type III secretion system ATPase, FliI/YscN VFG0192 Protein 6e-102 54
Sgly_0598 YP_004264962.1 type III secretion system ATPase, FliI/YscN VFG0048 Protein 3e-102 54
Sgly_0598 YP_004264962.1 type III secretion system ATPase, FliI/YscN VFG0390 Protein 7e-103 54
Sgly_0598 YP_004264962.1 type III secretion system ATPase, FliI/YscN VFG1254 Protein 5e-98 52
Sgly_0598 YP_004264962.1 type III secretion system ATPase, FliI/YscN VFG0730 Protein 9e-87 50
Sgly_0598 YP_004264962.1 type III secretion system ATPase, FliI/YscN VFG0812 Protein 2e-86 50
Sgly_0598 YP_004264962.1 type III secretion system ATPase, FliI/YscN VFG0515 Protein 8e-90 49
Sgly_0598 YP_004264962.1 type III secretion system ATPase, FliI/YscN VFG2499 Protein 8e-93 49
Sgly_0598 YP_004264962.1 type III secretion system ATPase, FliI/YscN VFG2331 Protein 8e-93 47
Sgly_0598 YP_004264962.1 type III secretion system ATPase, FliI/YscN VFG1778 Protein 1e-71 45
Sgly_0598 YP_004264962.1 type III secretion system ATPase, FliI/YscN VFG1008 Protein 2e-69 44
Sgly_0598 YP_004264962.1 type III secretion system ATPase, FliI/YscN VFG2459 Protein 3e-73 43
Sgly_0598 YP_004264962.1 type III secretion system ATPase, FliI/YscN VFG0555 Protein 2e-71 42