Gene Information

Name : Asphe3_39850 (Asphe3_39850)
Accession : YP_004243207.1
Strain :
Genome accession: NC_015146
Putative virulence/resistance : Virulence
Product : 2,3-dihydroxybenzoate-AMP ligase
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : -
Position : 5628 - 7301 bp
Length : 1674 bp
Strand : -
Note : PFAM: AMP-binding enzyme; TIGRFAM: 2,3-dihydroxybenzoate-AMP ligase

DNA sequence : 
ATGACCAGCCACACCAGTGAAGGCACTGTCCCCTGGCCAGCAGAACTTGCTGCAGAATTCCGCCGGAAGGGCTACTGGGA
GGACCGGGCCCTGGGCAGCTACGTCCTGGACACCGCCGACCGCTTTCCCGACAAGGTGGCGTTAGTAGAGGGCGATGTCC
GCATCACCTATGCCGAGCTGGCCAACCGCATGGACGCCGCAGCCGAACGCCTTCTCCAGCTTGGACTCAAAGCAGATGAC
CGCATCATGCTCCAGCTGCCGAACGGATGGCAGTTCACCATACTCACGCTGGCGTGTTTCCGTGCCGGCATCGTCCCTGT
CATGGCGTTGCCTGCGCACAGGAAATACGAGCTGTCCTTCCTGACAGAACTCTCCGAGTCCCGCGCCATCGCCGTCCCGG
ACGCAATCAAGGATTTCGATCACCAGGCCATGGCCGAGGAACTCGCTGAGTCCATTCCGTCCCTTGATCTGATCCTCGTT
TCCGGCGCCGCTAACCCCCTCAACATTCGCCTGAACGACATCCTTGCCCCCGGGACGGACGCTGCCGGGGCCCGGGCCAG
GCTGGATGCATCGGCGCCATCTCCGGATGCGCCTGCTCTGTTCCTGCTCTCAGGGGGCACTACCGGGCTGCCCAAGCTCA
TCACCCGCACTCACAATGACTACGCGTACAACATCAAGGCGACGTCTCTGCCAACGGCCGTTGCTGAGGACACCGTTTAC
CTCGGTACTCTGCCGGCGAGCCATAACTTTCCCCTCGCCTGCCCGGGGATCTTGGGCATACTGTTCGCCGGCGGCCGAGT
GATCATGCTGCCCAGCCCCGAGCCACGCAAAGCCTTTGCTGCCATTGAACGCGAAGGCGTCACACTCTCCACCGCGGTCC
CCGCGGTGGCGCAGCGCTGGATTGAACACCAGGAGGAAGTGGGCGGCAACCAGCTGGCGAGCCTGCAGGTCATCCAGGTT
GGCGGTTCCAGGCTCCCGGATGAGATCGCCTACAAGGTCAAGCCCGTGTTGGGTGCAACCTTGCAGCAGGTCTTTGGCAT
GGCCGAGGGTCTCATCAACACCACGCGGCTGGATGACCCCGAGGACGTTATCTGCACGACCCAGGGCCGTCCCGTATCCG
AGGCTGACGAAATTCGGATTGTGGATGAGGCAGGCAATGACTTACCCGACGGGACTGCTGGAGCCATCCTCACGCGCGGC
CCCTACACACCTCGCGGCTACTACCGCGCGCCGGAGGCCAACGCCCGGGCGTTCACCCCTGATGGCTGGTACGCCAGCGG
GGACATCGTGGAGCGCCGCCCGGACGGAAATCTCATTGTCCAGGGACGCGACAAGGACATGATCAACCGCGGCGGAGAAA
AAATATCGGCCGAGGAAATCGAAAGCCTGGTCTACCGCATCGAGGACATCACCATGGCGGCCGCCATCGCCATGCCGGAT
CCGGTGCTCGGTGAAAAACTCTGCCTCTACATCACCGTGAAACCGGGCACGGAGGTAACTCTTGAACAGATTCAAAAGAT
GCTCCGCGACACCGGTGTGGCTGCGTTCAAAATTCCCGAACGCCTGGTGGTCGTCGATGAACTCCCTGCCACAAAAGTCG
GCAAGATCAACAAAAAAGAGCTCCGTGCCGATATCGCAGAACGGCTGGCCACCGCCAGCGCCCACTCTAAGTAG

Protein sequence : 
MTSHTSEGTVPWPAELAAEFRRKGYWEDRALGSYVLDTADRFPDKVALVEGDVRITYAELANRMDAAAERLLQLGLKADD
RIMLQLPNGWQFTILTLACFRAGIVPVMALPAHRKYELSFLTELSESRAIAVPDAIKDFDHQAMAEELAESIPSLDLILV
SGAANPLNIRLNDILAPGTDAAGARARLDASAPSPDAPALFLLSGGTTGLPKLITRTHNDYAYNIKATSLPTAVAEDTVY
LGTLPASHNFPLACPGILGILFAGGRVIMLPSPEPRKAFAAIEREGVTLSTAVPAVAQRWIEHQEEVGGNQLASLQVIQV
GGSRLPDEIAYKVKPVLGATLQQVFGMAEGLINTTRLDDPEDVICTTQGRPVSEADEIRIVDEAGNDLPDGTAGAILTRG
PYTPRGYYRAPEANARAFTPDGWYASGDIVERRPDGNLIVQGRDKDMINRGGEKISAEEIESLVYRIEDITMAAAIAMPD
PVLGEKLCLYITVKPGTEVTLEQIQKMLRDTGVAAFKIPERLVVVDELPATKVGKINKKELRADIAERLATASAHSK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 2e-86 43
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 1e-86 43
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 3e-84 42
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 3e-84 42
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 3e-84 42
ybtE CAA21394.1 - Virulence HPI Protein 3e-84 42
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 2e-84 42
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 3e-84 42
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 3e-84 42

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Asphe3_39850 YP_004243207.1 2,3-dihydroxybenzoate-AMP ligase VFG1817 Protein 3e-95 44
Asphe3_39850 YP_004243207.1 2,3-dihydroxybenzoate-AMP ligase VFG0168 Protein 4e-98 42