Gene Information

Name : BSn5_05420 (BSn5_05420)
Accession : YP_004204739.1
Strain : Bacillus subtilis BSn5
Genome accession: NC_014976
Putative virulence/resistance : Virulence
Product : pyruvate kinase
Function : -
COG functional category : G : Carbohydrate transport and metabolism
COG ID : COG0469
EC number : 2.7.1.40
Position : 993299 - 995056 bp
Length : 1758 bp
Strand : -
Note : COG0469 Pyruvate kinase

DNA sequence :
ATGAGAAAAACTAAAATTGTTTGTACCATCGGTCCGGCAAGTGAAAGTATTGAAATGCTTACGAAATTAATGGAGTCAGG
AATGAACGTGGCTCGATTAAACTTTTCTCACGGAGATTTTGAGGAGCACGGTGCAAGAATTAAAAATATCCGCGAAGCAA
GCAAAAAACTTGGCAAGAACGTTGGAATTCTGCTTGATACAAAAGGTCCTGAAATCCGCACACATACAATGGAAAACGGC
GGTATTGAGCTTGAAACAGGCAAAGAGCTCATTATTTCAATGGACGAGGTTGTAGGAACAACAGATAAAATTTCAGTGAC
ATATGAAGGTTTAGTCCATGACGTTGAACAAGGTTCAACGATTCTGTTAGATGACGGCCTAATCGGTCTTGAGGTACTTG
ATGTAGATGCCGCTAAACGCGAAATCAAAACAAAAGTATTAAACAACGGAACACTCAAAAATAAAAAAGGTGTTAACGTA
CCGGGCGTAAGTGTCAATCTTCCGGGGATTACTGAAAAGGATGCGCGAGACATCGTTTTCGGTATTGAGCAAGGAGTAGA
CTTCATCGCACCATCTTTCATTCGACGTTCCACGGATGTGCTCGAAATCCGTGAGCTTCTTGAAGAGCACAACGCTCAGG
ATATTCAAATCATCCCTAAAATCGAAAACCAAGAGGGCGTTGACAACATCGATGCGATTCTCGAAGTGTCTGACGGCTTA
ATGGTTGCACGCGGAGACTTAGGTGTGGAAATTCCAGCTGAAGAAGTGCCGCTTGTGCAAAAAGAACTGATCAAAAAATG
CAACGCGCTGGGCAAACCTGTTATTACAGCGACACAAATGCTTGACAGCATGCAGCGCAACCCGCGTCCGACTCGTGCGG
AAGCAAGTGACGTTGCAAACGCGATCTTCGACGGAACAGATGCGATCATGCTTTCAGGTGAAACTGCTGCCGGAAGTTAC
CCGGTTGAAGCAGTTCAAACAATGCATAACATCGCGTCCCGTTCTGAAGAAGCTTTAAATTATAAAGAAATTCTCTCAAA
ACGCAGAGATCAAGTGGGTATGACAATTACAGACGCAATTGGACAATCTGTCGCACATACGGCGATTAACCTGAATGCTG
CTGCGATCGTAACGCCGACAGAAAGCGGCCATACAGCACGTATGATTGCAAAATACCGTCCGCAGGCTCCGATTGTTGCG
GTTACTGTAAATGACTCTATTTCCAGAAAGCTTGCCCTCGTATCTGGCGTATTCGCGGAAAGCGGCCAAAATGCGAGCTC
AACAGATGAGATGCTTGAGGATGCTGTCCAAAAATCATTGAACAGCGGAATTGTAAAACACGGCGATCTTATCGTTATTA
CAGCAGGCACTGTCGGTGAGTCCGGCACTACGAACTTAATGAAGGTTCATACTGTCGGCGATATCATCGCTAAAGGCCAA
GGCATTGGACGCAAATCAGCTTACGGTCCGGTTGTCGTTGCACAAAATGCAAAAGAAGCTGAGCAAAAAATGACTGACGG
TGCGGTACTTGTTACCAAAAGCACTGACCGTGATATGATTGCATCCCTTGAAAAAGCGTCTGCTCTTATTACAGAAGAAG
GCGGTTTGACTAGCCATGCTGCGGTAGTCGGATTAAGCCTTGGCATCCCGGTTATCGTGGGTCTGGAAAATGCGACATCT
ATTTTGACAGATGGCCAGGATATTACAGTTGACGCTTCCAGAGGTGCAGTCTATCAAGGCCGTGCGAGCGTTCTTTAA

Protein sequence :
MRKTKIVCTIGPASESIEMLTKLMESGMNVARLNFSHGDFEEHGARIKNIREASKKLGKNVGILLDTKGPEIRTHTMENG
GIELETGKELIISMDEVVGTTDKISVTYEGLVHDVEQGSTILLDDGLIGLEVLDVDAAKREIKTKVLNNGTLKNKKGVNV
PGVSVNLPGITEKDARDIVFGIEQGVDFIAPSFIRRSTDVLEIRELLEEHNAQDIQIIPKIENQEGVDNIDAILEVSDGL
MVARGDLGVEIPAEEVPLVQKELIKKCNALGKPVITATQMLDSMQRNPRPTRAEASDVANAIFDGTDAIMLSGETAAGSY
PVEAVQTMHNIASRSEEALNYKEILSKRRDQVGMTITDAIGQSVAHTAINLNAAAIVTPTESGHTARMIAKYRPQAPIVA
VTVNDSISRKLALVSGVFAESGQNASSTDEMLEDAVQKSLNSGIVKHGDLIVITAGTVGESGTTNLMKVHTVGDIIAKGQ
GIGRKSAYGPVVVAQNAKEAEQKMTDGAVLVTKSTDRDMIASLEKASALITEEGGLTSHAAVVGLSLGIPVIVGLENATS
ILTDGQDITVDASRGAVYQGRASVL

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
STY1744 NP_456147.1 pyruvate kinase Not tested SPI-2 Protein 3e-117 53
pykF YP_216386.1 pyruvate kinase Not tested SPI-2 Protein 3e-117 53
pykF NP_805051.1 pyruvate kinase Not tested SPI-2 Protein 3e-117 53
pykF CAA68205.1 pyruvate kinase like protein Not tested SPI-2 Protein 5e-117 53

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
BSn5_05420 YP_004204739.1 pyruvate kinase VFG0479 Protein 1e-117 53