Gene Information

Name : Varpa_4094 (Varpa_4094)
Accession : YP_004156376.1
Strain : Variovorax paradoxus EPS
Genome accession: NC_014931
Putative virulence/resistance : Resistance
Product : heavy metal translocating p-type ATPase
Function : -
COG functional category : P : Inorganic ion transport and metabolism
COG ID : COG2217
EC number : -
Position : 4418202 - 4420496 bp
Length : 2295 bp
Strand : -
Note : KEGG: vap:Vapar_3562 heavy metal translocating P-type ATPase; TIGRFAM: heavy metal translocating P-type ATPase; cadmium-translocating P-type ATPase; ATPase, P-type (transporting), HAD superfamily, subfamily IC; PFAM: E1-E2 ATPase-associated domain protein

DNA sequence :
ATGCCGAACCAGAACGCCCTGAAGCCCGTGACGCCGGCTCTGCCTCACGACCACGACCATGGTGACGATCACTCGCACGG
ACCTGCGCACACCCACGCTCATGCCCATGCCGAAACGGCCTGCTGCGGCAACCCGGTCTGCGGTTCGTCACCCGCGGTGG
ACACCACCGACATTCCCAAGGGCGCCTTGCTCTTTCGCATTCCCACGATGGACTGCGCGGTCGAGGAATCCGAAATCCGC
CGCGCCCTCGAACCGGTGGCCGGCATCCAGGGCCTGCGCTTTCGCCTCGGCGAGCGCACGATGGCCATCACGGCCGACGA
TGGCGCGCTGCCCCAGGCGCTCGATGCGATCCGCAAGGCCGGCTTCAAGCCCGAGCCTGTCGGCGACACGGCTGCCCCGG
CCGCCCCTGCACGCATCGCGGGAATGAGCGCCGGCCTCGCGCGCCTCGTCGCCGCACTGGTGCTGGCCATCGCGGCCGAA
TCGATCTCCTTCCTGGCCCTCGACGGCAAGGGCTTCATGGCCGCCGAGATGGTGCTCGCGCTCGCGGCCATTGGCCTGGC
CGGCCTCGACACCTACAAGAAGGGCTTCGGCGCGCTGGTGCGCGGGCGTCTCAACATCAACGCGCTGATGGCCGTTGCCG
TGACTGGCGCCTTCGTCATCGGCCAGTGGCCCGAGGCGGCGATGGTGATGGCGCTCTATGCGATTGCCGAGCTGATCGAA
GCCCGCGCCGTCGACCGCGCCCGCAACGCGATCCAGAGCCTGCTCGCGCTCGCGCCTGAGCAGGCTGAAGTCAAGCAACC
CGATGGCAGTTGGCAGACCATGAGGGCCGACGCCGTGGCGCTCGATGCCGTCGCACGCATTCGCCCCGGCGAGCGCGTGC
CGCTCGATGGCGTGGTGACCGCGGGCCTGAGCGCCATCGACCAGGCGCCGGTCACGGGCGAGAGCATCCCGGTCGACAAG
ACCGTGGGCGACCCGGTCTTCGCGGGCACCATCAACCAGACCGCCGCGCTGGAATTCCGTGTCACGGCGGTGGCGGCCAA
CACCACGCTCGCGCGCATCATCCACGCGGTCGAAGAGGCGCAGGGCACGCGCGCGCCCACGCAGCGTTTCGTCGACCGTT
TCGCTGCCATCTACACGCCCGCGGTCTTCGTGCTGGCGTTGGCGATCGCGCTGTTCACGCCGCTCTTCATGGACTGGACC
TGGCTGCAGGCCATCTACAAGGCGCTGGTGCTGCTTGTCATCGCCTGCCCGTGTGCGCTGGTGATCTCGACGCCCGTCAC
GGTCGTGAGCGCGCTCGCATCGGCCGCGCGGCGCGGCATCCTCATCAAGGGCGGCACGTACCTCGAAGAGGCACGCAAGC
TGAAGGCCGTGGCGCTCGACAAGACGGGCACCATCACCGAAGGCAAGCCGAAGCTGGTCGAGTCCAGCCTGCTCGATGCA
TCGGGCAGCAATGAAGCGGCGGTGTTCGCGATCGCCGCGAGCATCGCGGGCCGCTCGGACCATCCCGTGTCGAAGGCCAT
CGCCGAGGGCCTGAAGGGCGAGCGCGAAGAAGTCGGCGACTTCACCGCGTTGCCGGGTCGCGGCGTGCAGGCCGTGCTTT
CGGGCCAGAGCTACATGCTCGGCAACCATCGGCTGATCGAAGAGAAGGGCTTGTGCACTCCGGCACTCGAAGCCGAACTC
AAGCGCCATGAGGAGGCGGGCCGCACCGTGACCCTGCTTGCATCGAGTCGGGCCGTGCTCGCGCTGTTCGCTGTCGCCGA
CACGATCAAGGAATCGTCGCAAGCCGCGGTGGCCGAACTGCGCGCCCTCGGCGTCGTGCCCGTGATGCTCACCGGCGACA
ACGCCGCCACGGCCAGGACCATCGGTGGCCACGCCGGCATCGAGGACGTGCGCGGCAACCTGCTGCCCGAAGAGAAGCTC
GGCGCCATCAAGGCGATGCAGGAACGCTACGGCGCGGTGGCGATGACCGGCGACGGCATCAACGACGCGCCGGCGCTCGC
GCAGGCCGACATCGGTTTCGCCATGGGCGGCGCGGGCACCGACACCGCGATGGAAGCGGCCGACGTGGTCATCATGAACG
ACGACCTGCGCCGCATTCCCGAGACCATCCGCCTCTCGCGCCGCGCGCACGCGGTGCTGTGGCAGAACATCACGCTGGCA
CTCGGCATCAAGGGCATCTTCTTCGTGCTCGCGGTGTTCGGCAGCGCGACGATGTGGATGGCGGTGTTCGCGGACATGGG
CGCGAGCCTTCTGGTGGTGGCGAACGGGCTGCGGCTGATGCGGGCGCCCAAGTAG

Protein sequence :
MPNQNALKPVTPALPHDHDHGDDHSHGPAHTHAHAHAETACCGNPVCGSSPAVDTTDIPKGALLFRIPTMDCAVEESEIR
RALEPVAGIQGLRFRLGERTMAITADDGALPQALDAIRKAGFKPEPVGDTAAPAAPARIAGMSAGLARLVAALVLAIAAE
SISFLALDGKGFMAAEMVLALAAIGLAGLDTYKKGFGALVRGRLNINALMAVAVTGAFVIGQWPEAAMVMALYAIAELIE
ARAVDRARNAIQSLLALAPEQAEVKQPDGSWQTMRADAVALDAVARIRPGERVPLDGVVTAGLSAIDQAPVTGESIPVDK
TVGDPVFAGTINQTAALEFRVTAVAANTTLARIIHAVEEAQGTRAPTQRFVDRFAAIYTPAVFVLALAIALFTPLFMDWT
WLQAIYKALVLLVIACPCALVISTPVTVVSALASAARRGILIKGGTYLEEARKLKAVALDKTGTITEGKPKLVESSLLDA
SGSNEAAVFAIAASIAGRSDHPVSKAIAEGLKGEREEVGDFTALPGRGVQAVLSGQSYMLGNHRLIEEKGLCTPALEAEL
KRHEEAGRTVTLLASSRAVLALFAVADTIKESSQAAVAELRALGVVPVMLTGDNAATARTIGGHAGIEDVRGNLLPEEKL
GAIKAMQERYGAVAMTGDGINDAPALAQADIGFAMGGAGTDTAMEAADVVIMNDDLRRIPETIRLSRRAHAVLWQNITLA
LGIKGIFFVLAVFGSATMWMAVFADMGASLLVVANGLRLMRAPK

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
cadA BAC57487.1 Probable cadmium-transporting ATPase (Cadmium efflux ATPase) Not tested Type-IIIinv SCCmec Protein 1e-117 45
unnamed BAB47609.1 cadmium resistance protein B Not tested Type-III SCCmec Protein 1e-117 45
SE0075 NP_763630.1 cadmium resistance protein B Not tested SCCpbp4 Protein 3e-117 43

• Homologs from CARD and BacMet (resistance genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Varpa_4094 YP_004156376.1 heavy metal translocating p-type ATPase BAC0298 Protein 4e-153 62
Varpa_4094 YP_004156376.1 heavy metal translocating p-type ATPase BAC0461 Protein 1e-79 41