Gene Information

Name : Pat9b_0484 (Pat9b_0484)
Accession : YP_004114366.1
Strain : Pantoea sp. At-9b
Genome accession: NC_014837
Putative virulence/resistance : Virulence
Product : 2,3-dihydroxybenzoate-AMP ligase
Function : -
COG functional category : Q : Secondary metabolites biosynthesis, transport and catabolism
COG ID : COG1021
EC number : -
Position : 562857 - 564473 bp
Length : 1617 bp
Strand : -
Note : KEGG: esa:ESA_00797 enterobactin synthase subunit E; TIGRFAM: 2,3-dihydroxybenzoate-AMP ligase; PFAM: AMP-dependent synthetase and ligase

DNA sequence : 
ATGAGTATTCCCTATAACCGCTGGCCTGACGATCTCGCCGCACGTTACCGTGAAAAAGGCTATTGGCTCGACGTGCCAAT
GACTGACATCCTGGCGCGTCAGCAGCAGAATGACGCGATCGCGGTGATCGACGGCGAACGCCAAATCAGCTACCGCGAAA
TGCATCAGCTGACCGACAATCTGGCGGCAGCGTTGCAACGTCGCGGTTTGAAAACCGGCGATACCGCGCTGGTACAGCTC
GGCAACGTGGCGGATTTCTATCTGACCCTGTTTGCCTTATTTAAAATCGGCGTGGCCCCCGTCAATGCCCTTTTCAGCCA
TCAGCGAACCGAAATGACCGCCTACGCGGCACAGATTGAACCGGTGCTGCTGATTGCCGATCGTACCCATCCGCTGTTCG
CGGATGACAGCTTTATCGATGTGCTGTGCCGCAACCATCCCTCATTGCAGCAAGTGATTCTGCGCAATGACAGCCAAGCG
GAAAACACCCTGGAAGCGCTGCTGGCAGAAAAAGCGGGCGATTTTAGCCCGACGCCCAGCGCCGGTGATGAAGTGGCCTT
CTTCCAGCTTTCTGGCGGCAGTACCGGTACACCGAAACTGATCCCACGCACCCACAATGATTACTACTACAGCATTCGCG
GCAGCGTGGATATTTGCGCCGTAACCGATCGTACCCGCTATCTGGTGGCGCTGCCTGCGGCACATAACTTCCCGATGAGT
TCGCCCGGCGCACTCGGCGTGTTTTACGCCGGTGGTCAGGTGGTACTGGCTGCCGATCCCAGCGCGACGCTGTGCTTTCC
GTTAATCGAAAAGCATCAGATCACCGATACCGGCCTGGTGCCGCCAGCAGTGAGCCTGTGGTTACAGGCGATTCAGGAAT
GGGGCAGCAACAAACAGCTGGCCTCACTGGAACGGATGCAGGTCGGTGGCGCGAAGTTGGGTGAAACCCTTGCGTCCCGC
ATCCAGCAGGAGATCGGCTGCCGTCTGCAACAGGTGTTCGGGATGGCGGAAGGGCTGGTGAACTACACCCGCTTTGACGA
CGATGAGCAAACTATCCTCACCACGCAGGGACGCCCCATTTCTGACGATGATGAAGTGTGGGTCGCCGATGCCGACGGCA
ATGCGCTGCCGGTGGGTGAAGTGGGTCGTCTGATGACACGCGGTCCTTATACCTTCCGGGGTTACTATCGCAGCCCGGAG
CATAACGCCGCCAGCTTTGATGCCGACGGGTTTTACTGTTCCGGCGATCTGATTGAGATCAACCCGCAGGGTTACATCAC
CGTGCAAGGGCGCGAGAAAGATCAGATCAACCGTGGCGGCGAAAAGATCGCCGCAGAAGAGATTGAAAACCTGCTGCAAC
GCCACCCGGATGTGATTCACGCCGCACTGGTGTCCATGAATGATGAGCTGATGGGTGAAAAGAGCTGTGCGTTTATTGTC
GCCAGCCAGCCGATTAAGCCGGTGGTGCTGCGTCGCCATCTGCGTGAACTGGGCGTTGCCGAGTTCAAATTACCCGATCG
TATTACCTGCGTAGACGCCCTGCCGCTGACCCCGGTCGGTAAGGTGGATAAAAAACGTCTGCGCCAACAACTTGCCGATC
AACAAGCGCAAGCCTGA

Protein sequence : 
MSIPYNRWPDDLAARYREKGYWLDVPMTDILARQQQNDAIAVIDGERQISYREMHQLTDNLAAALQRRGLKTGDTALVQL
GNVADFYLTLFALFKIGVAPVNALFSHQRTEMTAYAAQIEPVLLIADRTHPLFADDSFIDVLCRNHPSLQQVILRNDSQA
ENTLEALLAEKAGDFSPTPSAGDEVAFFQLSGGSTGTPKLIPRTHNDYYYSIRGSVDICAVTDRTRYLVALPAAHNFPMS
SPGALGVFYAGGQVVLAADPSATLCFPLIEKHQITDTGLVPPAVSLWLQAIQEWGSNKQLASLERMQVGGAKLGETLASR
IQQEIGCRLQQVFGMAEGLVNYTRFDDDEQTILTTQGRPISDDDEVWVADADGNALPVGEVGRLMTRGPYTFRGYYRSPE
HNAASFDADGFYCSGDLIEINPQGYITVQGREKDQINRGGEKIAAEEIENLLQRHPDVIHAALVSMNDELMGEKSCAFIV
ASQPIKPVVLRRHLRELGVAEFKLPDRITCVDALPLTPVGKVDKKRLRQQLADQQAQA

• Homologs from PAI DB

GeneGenBank Accn Product Virulance or Resistance PAI or REI Alignment Type E-val Identity
ybtE CAA21394.1 - Virulence HPI Protein 9e-92 43
irp5 CAA73130.1 Irp5 protein Virulence HPI Protein 3e-91 43
ybtE AAA93464.1 yersiniabactin 2,3-dihydoxybenzoate-AMP ligase Virulence HPI Protein 7e-92 43
irp5 YP_001006819.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 3e-91 43
ybtE YP_853079.1 yersiniabactin siderophore biosynthetic protein Virulence PAI IV APEC-O1 Protein 9e-92 43
irp5 YP_002346898.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 9e-92 43
ybtE NP_669710.1 salicyl-AMP ligase Virulence HPI Protein 9e-92 43
ybtE NP_993003.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 9e-92 43
ybtE YP_070120.1 yersiniabactin siderophore biosynthetic protein Virulence HPI Protein 9e-92 43

• Homologs from VFDB (virulence genes)

GeneGenBank Accn Product ID of source DB Alignment Type E-val Identity
Pat9b_0484 YP_004114366.1 2,3-dihydroxybenzoate-AMP ligase VFG0932 Protein 0.0 71
Pat9b_0484 YP_004114366.1 2,3-dihydroxybenzoate-AMP ligase VFG0168 Protein 3e-107 44